FastQCFastQC Report
Thu 26 May 2016
SRR1511746_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511746_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315434
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66760.28832607623452017No Hit
TATCAACGCAGAGTACTTTTTTTTT41800.18052771100363907No Hit
GGTATCAACGCAGAGTACTTTTTTT40120.17327205180540667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.013.1950181
CCCGTAC951.043969E-611.99668616
TCCAACG1604.5474735E-1111.27813518
AACCGTG600.005889226711.0802727
CAGCGAA600.005889226711.0802729
GTATCGC1002.4070909E-510.4471146
ATAGGAC1659.295036E-1010.3634593
GTCTTAA2500.010.3003181
GTATTAG2053.6379788E-1210.2351861
ATCGCGC750.002654483810.1305358
TCGCGCG750.002654483810.1305359
CGACTAA1054.1190528E-59.94963217
AAGGCGT2107.2759576E-129.9496326
TAGGACC3550.09.9012394
GTATCAA29750.09.8739431
GTATTAT2251.8189894E-129.7492721
TAGGACT2251.8189894E-129.7109464
AGGACTG5000.09.4998385
ATGACCG900.00111412089.4998385
TACACTA1901.0531949E-99.4998385
Produced by FastQC (version 0.11.5)