FastQCFastQC Report
Thu 26 May 2016
SRR1511745_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511745_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471898
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55200.22331018512899803No Hit
GGTATCAACGCAGAGTACTTTTTTT32670.1321656476116733No Hit
TATCAACGCAGAGTACTTTTTTTTT32550.13168019068747983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG602.9221084E-413.18240451
GTACTAG1154.0663508E-812.0361091
GGCGAGG7250.011.8696919
TCCAACG3250.011.34794518
CCGGACA752.1652358E-411.3477154
TAGGACC4950.011.0802614
TGGCGAG13300.010.94977218
TTAGGAC9650.010.7793493
GGTATCA14250.010.6846861
TATGGCG14000.010.26718816
ATGGCGA14350.010.21446917
GCGTGCG1304.0941268E-710.1838479
AGGACGT17200.010.171165
AAAGGCG2350.010.0600325
GGACGTG16550.010.0563846
GTCCTAC12000.09.8868041
TAGGACT3450.09.86757854
CCAACGA2500.09.83488619
ACGTGAA9400.09.758238
TAGGACG17550.09.7527844
Produced by FastQC (version 0.11.5)