FastQCFastQC Report
Thu 26 May 2016
SRR1511745_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511745_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2471898
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69420.28083683064592474No Hit
TATCAACGCAGAGTACTTTTTTTTT44810.18127770644257976No Hit
GGTATCAACGCAGAGTACTTTTTTT42830.17326766719338743No Hit
GTCCTACAGTGGACATTTCTAAATT25310.10239095626114023No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG550.00307613912.0873312
TCCAACG2800.011.87148618
CCAACGA2200.011.65587619
GGTATCA15350.011.4316551
TATACCG600.00587434211.0840895
GCCGACT600.00589028611.08005212
TAGGACC4650.011.03301054
TGGACCG951.3599558E-511.0007515
TCTATAC3250.010.2314673
CGCCCTA1606.206392E-910.09076216
CTTATAC2002.5465852E-119.9756793
GTCCTAA8650.09.9226131
GATATAC7950.09.8366311
GTATCAA30700.09.6920561
GTAGGAC13950.09.670913
CTACACT4850.09.5985934
ACTGTTC8450.09.5533858
GTATTAA3100.09.5367351
TGTAGGA15800.09.5006482
TAAGCCC1304.2368265E-69.5006484
Produced by FastQC (version 0.11.5)