FastQCFastQC Report
Thu 26 May 2016
SRR1511744_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511744_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2569983
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54280.21120762277415844No Hit
TATCAACGCAGAGTACTTTTTTTTT31750.12354167323285796No Hit
GGTATCAACGCAGAGTACTTTTTTT30950.12042881217502216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13000.011.7859191
TAGGACC3800.011.1986914
CCCGTTT1950.011.15402412
CGTTTCC2000.010.87517414
TGGCGAG6900.010.82720518
CCAACGA1951.8189894E-1210.66906619
GTCCTAC7150.010.6453481
TAGGACG7150.010.5808934
TTAGGAC5550.010.393823
GTAGGAC7300.010.3634763
AGGACGT7250.010.3045125
GGACGTG7300.010.23393256
GTATCAA26000.010.075061
TCCAACG3100.010.0667818
TGTAGGA8450.010.0604372
ATGGCGA7250.010.04363817
TATGGCG7250.010.04363816
GGTCTAT1802.1100277E-99.8849661
GCCGGTT1554.3643013E-89.76172611
ATCCCGT1951.7826096E-109.69915210
Produced by FastQC (version 0.11.5)