##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511744_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2569983 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.98614426632394 31.0 28.0 34.0 19.0 34.0 2 26.3687938791813 31.0 25.0 34.0 10.0 34.0 3 28.007979430214128 31.0 27.0 34.0 16.0 34.0 4 32.45403763371198 35.0 32.0 37.0 24.0 37.0 5 32.88358872412775 35.0 33.0 37.0 26.0 37.0 6 33.121192241349455 37.0 35.0 37.0 27.0 37.0 7 33.15097025933635 37.0 35.0 37.0 26.0 37.0 8 33.16918166384758 37.0 35.0 37.0 26.0 37.0 9 34.722496997061846 39.0 35.0 39.0 27.0 39.0 10 34.59261676049997 39.0 35.0 39.0 27.0 39.0 11 34.608087679957414 39.0 35.0 39.0 27.0 39.0 12 34.495605612955416 39.0 35.0 39.0 27.0 39.0 13 34.483132378696666 39.0 35.0 39.0 27.0 39.0 14 35.595180590688734 40.0 36.0 41.0 27.0 41.0 15 35.5152337583556 40.0 36.0 41.0 26.0 41.0 16 35.447070272449274 40.0 36.0 41.0 26.0 41.0 17 35.41087509139165 40.0 36.0 41.0 26.0 41.0 18 35.3404306565452 40.0 36.0 41.0 25.0 41.0 19 35.37062929988253 40.0 36.0 41.0 25.0 41.0 20 35.285727181852955 40.0 36.0 41.0 25.0 41.0 21 35.19603787262406 40.0 36.0 41.0 25.0 41.0 22 35.07866744643836 40.0 35.0 41.0 24.0 41.0 23 34.96197367842511 40.0 35.0 41.0 24.0 41.0 24 34.825299622604504 39.0 35.0 41.0 22.0 41.0 25 34.70802647332687 39.0 35.0 41.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2145908351460406 1101 2 3.7740016649155876 1101 3 2.427764608344205 1101 4 1.4493769897524018 1101 5 1.0318365717229625 1101 6 0.7917194197356565 1101 7 0.7835201507085614 1101 8 0.5226800569883636 1101 9 0.6958419384333681 1101 10 0.8527878154143735 1101 11 0.7580255947318619 1101 12 0.8275001797748018 1101 13 0.8450479639253885 1101 14 0.9546018533704697 1101 15 0.9020068294717518 1101 16 0.8167670419682267 1101 17 0.9185634695443596 1101 18 0.8285946786642597 1101 19 0.8093733263570968 1101 20 0.9613594220134019 1101 21 1.04656167157259 1101 22 0.996271794252138 1101 23 1.0234782278798136 1101 24 1.030572460660963 1101 25 0.9244997591388469 1102 1 1.7172404830676662 1102 2 4.308082734516823 1102 3 2.9104434045362275 1102 4 1.9164039830771387 1102 5 1.3939325194119476 1102 6 1.1399070052223124 1102 7 1.022585067959433 1102 8 1.1990058551560736 1102 9 1.2388173221734675 1102 10 1.1771603287512704 1102 11 1.2782177862687334 1102 12 1.1336452820470413 1102 13 1.2482686919307255 1102 14 1.348673219871003 1102 15 1.359297404214857 1102 16 1.4982775692479677 1102 17 1.442048090714266 1102 18 1.3481857845449596 1102 19 1.3925704006189434 1102 20 1.3845383370586646 1102 21 1.5154797953280408 1102 22 1.25194732879946 1102 23 1.3739476007633513 1102 24 1.1836957679764737 1102 25 1.0593475295689387 1103 1 1.442181970169841 1103 2 3.9634217004343384 1103 3 2.639723288896114 1103 4 1.5553027366445136 1103 5 1.3327455575469855 1103 6 0.9366968789889256 1103 7 0.7940118437340686 1103 8 0.7147146089581824 1103 9 0.9181514980218566 1103 10 0.6960807610538708 1103 11 0.9207056842643411 1103 12 1.0662702641581134 1103 13 1.0639233435451914 1103 14 1.2061523835246248 1103 15 0.8532872910053655 1103 16 0.8983571891924029 1103 17 1.0672630418481432 1103 18 1.056942426165861 1103 19 1.1097884123692268 1103 20 1.0503244391948101 1103 21 1.111537869805062 1103 22 1.229495488832903 1103 23 1.115169151682636 1103 24 1.271736382855977 1103 25 0.9915335229706699 1104 1 1.4980028018699443 1104 2 3.962310204186899 1104 3 2.5220716990569443 1104 4 1.7286968711262034 1104 5 1.242723360016683 1104 6 1.0942021602158292 1104 7 0.9145726526211391 1104 8 1.0256862791216719 1104 9 1.104599234482066 1104 10 0.8920483836865003 1104 11 1.050066858275052 1104 12 1.085909877337258 1104 13 1.048165328660211 1104 14 1.1874059626829236 1104 15 1.2731186444860043 1104 16 1.1043390762668324 1104 17 1.0498975327141125 1104 18 1.0166425885350208 1104 19 1.1083063573825598 1104 20 1.015116319801109 1104 21 1.1189810792936683 1104 22 1.288918784973582 1104 23 1.404090327233888 1104 24 1.2064228404908022 1104 25 1.2136150084594846 1105 1 1.5191681847648333 1105 2 3.9963235178296017 1105 3 2.5073394732193286 1105 4 1.6883984886582155 1105 5 1.115469610320524 1105 6 0.9096903509453043 1105 7 0.9758832232612704 1105 8 0.903441996859371 1105 9 1.0150987922599626 1105 10 0.7926158169395592 1105 11 0.907296525760799 1105 12 0.9151794653251599 1105 13 0.8774992584316692 1105 14 0.761416375838877 1105 15 0.8744109951671106 1105 16 0.8729139364000531 1105 17 1.0738153141277067 1105 18 1.0200077659447189 1105 19 0.9876624493238992 1105 20 1.0001277481274258 1105 21 0.9936310293521871 1105 22 1.071165763463 1105 23 1.106355362274627 1105 24 1.1089327828283118 1105 25 1.0231296262999336 1106 1 0.9514207892000321 1106 2 3.6773847540416966 1106 3 2.088767648552679 1106 4 1.1945823752645524 1106 5 0.4607254098147209 1106 6 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0.8150976579552562 1107 19 0.7925863022179698 1107 20 0.8180186309750184 1107 21 0.8412134143372683 1107 22 0.8055804295103073 1107 23 0.8660956002989195 1107 24 0.9715141695520657 1107 25 0.8942373686274507 1108 1 1.608413125198929 1108 2 4.098987986797695 1108 3 2.67955908142471 1108 4 1.7800994823374268 1108 5 1.4260812036616102 1108 6 1.17088836608864 1108 7 1.0627495687406423 1108 8 0.9073394478561738 1108 9 0.8742161141467903 1108 10 0.9444417638310725 1108 11 0.8697331037786213 1108 12 1.0219655813355502 1108 13 1.0470848036562472 1108 14 1.0760808178649555 1108 15 1.0118042013533852 1108 16 1.1049501079783468 1108 17 1.1970249932386992 1108 18 1.248630961671715 1108 19 1.0172212064887916 1108 20 1.186774804856718 1108 21 1.3225064215749924 1108 22 1.3681802996417929 1108 23 1.4649829168346145 1108 24 1.470771729241605 1108 25 1.4552107333088031 1109 1 1.118772935295997 1109 2 3.239003865121507 1109 3 1.9941864754005003 1109 4 1.3488937988984233 1109 5 0.981012242144935 1109 6 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-1.852966362098222 2315 20 -1.9450135806307856 2315 21 -1.9863455685482307 2315 22 -1.8759722795041611 2315 23 -1.7134639553482245 2315 24 -1.8117733783653946 2315 25 -1.7695212679503456 2316 1 -0.47176427316028935 2316 2 1.7567393263672173 2316 3 0.48653997931469917 2316 4 -0.3875510489494687 2316 5 -0.6682487088501219 2316 6 -0.8964079739349771 2316 7 -1.395519434982905 2316 8 -1.3858976181081601 2316 9 -1.5064032664725886 2316 10 -1.5445994979374618 2316 11 -1.5416131257494285 2316 12 -1.6818760315969712 2316 13 -1.7601231399153363 2316 14 -1.9384501057568784 2316 15 -1.8443547911692448 2316 16 -2.077382710718112 2316 17 -1.8833189052870196 2316 18 -2.127084232225293 2316 19 -2.057264913451874 2316 20 -2.0282828647464015 2316 21 -2.496053806076148 2316 22 -2.147334488600265 2316 23 -2.4410807165017445 2316 24 -2.456392016103962 2316 25 -2.158493744032043 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 107927.0 3 4.0 4 975.0 5 4661.0 6 5553.0 7 5043.0 8 4589.0 9 4542.0 10 4294.0 11 4103.0 12 4124.0 13 4043.0 14 4151.0 15 4215.0 16 4440.0 17 4887.0 18 5293.0 19 6850.0 20 8787.0 21 11200.0 22 13827.0 23 16328.0 24 18795.0 25 21488.0 26 24374.0 27 27738.0 28 31991.0 29 38260.0 30 47507.0 31 59799.0 32 75871.0 33 100134.0 34 134563.0 35 189715.0 36 296790.0 37 378979.0 38 894143.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.772585266128225 18.181404312791173 12.866388610352676 25.17962181072793 2 16.98500760601648 20.778986244092117 38.738824850116735 23.497181299774667 3 18.983977715027684 23.992843532428036 29.227313954995033 27.795864797549246 4 12.618293584043164 16.460420166203434 37.76406303076713 33.157223218986275 5 13.920753561404881 37.084097443446126 35.215135664321515 13.780013330827481 6 32.367529279376555 36.45549406357941 17.914748852424317 13.26222780461972 7 28.518009652203922 31.3056156402591 21.783918415024537 18.392456292512442 8 25.731882273151225 36.001405456767614 20.36519307715265 17.901519192928514 9 26.4043380831702 15.652710543221493 19.83748530632304 38.10546606728527 10 15.158660582579731 28.84271997129942 33.757305009410565 22.241314436710283 11 34.98696294878215 22.673885391459788 23.500544556131306 18.838607103626757 12 23.154550049552856 24.449889357244775 31.16277422846766 21.232786364734707 13 28.819062227259867 21.410413998847464 25.7067459201092 24.06377785378347 14 21.668976020463948 21.115509324380746 27.384889316388474 29.83062533876683 15 23.693697584770014 28.960580673101727 23.59525335381596 23.750468388312296 16 23.255367837063513 27.751662170527975 26.117682490506745 22.875287501901763 17 22.53026576440389 27.552867081221937 27.038311148361682 22.87855600601249 18 22.346256765122572 27.408041220506128 28.083570980819715 22.16213103355158 19 23.589892847498543 26.853767847401265 27.15945092222436 22.396888382875836 20 23.633463723300892 26.73823134238631 27.540688012333153 22.087616921979638 21 23.827858247648322 26.356482066946175 26.946098697458147 22.869560987947356 22 23.530187699857663 26.61035956285599 27.182587703904424 22.676865033381922 23 23.534172644374536 26.42662448710391 26.931572998075847 23.107629870445706 24 23.192622824838004 26.493503259362615 27.401408804189604 22.912465111609777 25 23.517859845765518 26.44752903034767 27.15982946190695 22.874781661979863 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 222.0 1 222.0 2 405.5 3 589.0 4 589.0 5 589.0 6 1540.5 7 2492.0 8 2492.0 9 2492.0 10 3634.0 11 4776.0 12 4776.0 13 4776.0 14 7999.0 15 11222.0 16 11222.0 17 11222.0 18 19578.0 19 27934.0 20 27934.0 21 27934.0 22 43655.0 23 59376.0 24 59376.0 25 59376.0 26 82945.5 27 106515.0 28 106515.0 29 106515.0 30 136354.0 31 166193.0 32 166193.0 33 166193.0 34 191255.5 35 216318.0 36 216318.0 37 216318.0 38 240390.0 39 264462.0 40 264462.0 41 264462.0 42 285047.0 43 305632.0 44 305632.0 45 305632.0 46 318079.0 47 330526.0 48 330526.0 49 330526.0 50 329121.0 51 327716.0 52 327716.0 53 327716.0 54 301318.0 55 274920.0 56 274920.0 57 274920.0 58 239853.5 59 204787.0 60 204787.0 61 204787.0 62 169579.0 63 134371.0 64 134371.0 65 134371.0 66 104435.5 67 74500.0 68 74500.0 69 74500.0 70 55043.5 71 35587.0 72 35587.0 73 35587.0 74 24967.0 75 14347.0 76 14347.0 77 14347.0 78 9772.5 79 5198.0 80 5198.0 81 5198.0 82 3423.5 83 1649.0 84 1649.0 85 1649.0 86 1064.5 87 480.0 88 480.0 89 480.0 90 305.5 91 131.0 92 131.0 93 131.0 94 79.0 95 27.0 96 27.0 97 27.0 98 20.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.334775755326008 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 3.8910763222947385E-5 20 0.0 21 3.112861057835791E-4 22 0.0011284121334654744 23 7.00393738013053E-4 24 7.782152644589477E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2569983.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.29745354313457 #Duplication Level Percentage of deduplicated Percentage of total 1 82.45807867434526 60.43967190888957 2 11.844351778965818 17.363216485345806 3 3.058001984681977 6.724312752211216 4 1.1512230691775396 3.3752687772330203 5 0.523925273330746 1.9201194191017221 6 0.27746721583948125 1.2202584217642363 7 0.15851307118839747 0.8133023129987802 8 0.10869260408057368 0.6373512878462535 9 0.07387475551143069 0.48733483140985645 >10 0.32365048049495576 3.8794656881888017 >50 0.012828808168386725 0.6527056051169223 >100 0.007679568042527715 1.0600207783967495 >500 0.0010169252277332371 0.49830634338313 >1k 6.422685648326464E-4 0.7157220066218 >5k 5.3522380402720534E-5 0.21294338149216896 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5428 0.21120762277415844 No Hit TATCAACGCAGAGTACTTTTTTTTT 3175 0.12354167323285796 No Hit GGTATCAACGCAGAGTACTTTTTTT 3095 0.12042881217502216 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.1673228966884217E-4 0.0 11 0.0 0.0 0.0 1.5564305289178957E-4 0.0 12 0.0 0.0 0.0 1.5564305289178957E-4 3.891076322294739E-5 13 3.891076322294739E-5 0.0 0.0 1.5564305289178957E-4 7.782152644589478E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1300 0.0 11.785919 1 TAGGACC 380 0.0 11.198691 4 CCCGTTT 195 0.0 11.154024 12 CGTTTCC 200 0.0 10.875174 14 TGGCGAG 690 0.0 10.827205 18 CCAACGA 195 1.8189894E-12 10.669066 19 GTCCTAC 715 0.0 10.645348 1 TAGGACG 715 0.0 10.580893 4 TTAGGAC 555 0.0 10.39382 3 GTAGGAC 730 0.0 10.363476 3 AGGACGT 725 0.0 10.304512 5 GGACGTG 730 0.0 10.2339325 6 GTATCAA 2600 0.0 10.07506 1 TCCAACG 310 0.0 10.06678 18 TGTAGGA 845 0.0 10.060437 2 ATGGCGA 725 0.0 10.043638 17 TATGGCG 725 0.0 10.043638 16 GGTCTAT 180 2.1100277E-9 9.884966 1 GCCGGTT 155 4.3643013E-8 9.761726 11 ATCCCGT 195 1.7826096E-10 9.699152 10 >>END_MODULE