FastQCFastQC Report
Thu 26 May 2016
SRR1511744_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511744_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2569983
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71430.27793958170151317No Hit
TATCAACGCAGAGTACTTTTTTTTT42920.16700499575289018No Hit
GGTATCAACGCAGAGTACTTTTTTT42490.16533183293430345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1700.013.40746619
TCCAACG2250.013.08469418
GGTATCA14350.011.5679111
CGCCCTA1354.7566573E-911.255650516
TAGGACC3100.010.4200714
GTCTTAC2200.010.4074941
GTATCAA30200.010.2035931
TTAGGAC5700.010.1673643
TATCGCG750.002655631610.1300857
CGAAAAA856.6155335E-410.05559915
CGGCGAT951.6501012E-49.99679519
CCGGCGA951.6501012E-49.99679518
GTCTAGT1502.492161E-79.5402021
GGACCGT1002.765298E-49.4969566
CTGGTCG1106.8633846E-59.4969559
TAGGACG8150.09.4423664
TAGGACA4650.09.3984944
TGTAGAA9100.09.2918462
GATATAC6100.09.2274081
CGGTTTC1752.6851922E-89.22561413
Produced by FastQC (version 0.11.5)