FastQCFastQC Report
Thu 26 May 2016
SRR1511743_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511743_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60340.23355939643049714No Hit
GTCCTACAGTGGACATTTCTAAATT53040.2053031220860717No Hit
GTCCTAAAGTGTGTATTTCTCATTT50130.19403931957343087No Hit
CTGTAGGACGTGGAATATGGCAAGA48730.18862030805532193No Hit
CTTTAGGACGTGAAATATGGCGAGG40730.15765452795184204No Hit
GGTATCAACGCAGAGTACTTTTTTT35630.1379138431358736No Hit
TATCAACGCAGAGTACTTTTTTTTT35350.1368300408322518No Hit
TTGTAGAACAGTGTATATCAATGAG27570.10671581968161759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4550.014.13295418
CCAACGA3000.013.86969419
AGACCGA550.003176430512.0356856
CAGTACG550.003176430512.0356854
CCAACGT2150.011.87574119
GGTATCA12200.011.66821
TAGGACC7400.011.3734894
CGGTAGG1251.2947748E-711.0718251
GTCCTAT2700.010.9839531
TCCTATA3250.010.9501582
TGTAGAA14500.010.771852
GTATCAA25450.010.6429991
TAAGACG803.9354616E-410.6386854
TTGGACG909.971879E-510.5073424
GATATAC12050.010.5008421
CTTATAC2900.010.4348773
GGCGAGG12550.010.32315119
GTCCTAC25150.010.141051
CCGGTTT3750.010.087049512
GCCGGTT3850.010.07067411
Produced by FastQC (version 0.11.5)