FastQCFastQC Report
Thu 26 May 2016
SRR1511743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76570.29638122281543194No Hit
GTCCTACAGTGGACATTTCTAAATT60030.23235947245148725No Hit
CTGTAGGACGTGGAATATGGCAAGA55760.2158314873212549No Hit
GTCCTAAAGTGTGTATTTCTCATTT52420.202903274128052No Hit
CTTTAGGACGTGAAATATGGCGAGG47670.1845173421916108No Hit
TATCAACGCAGAGTACTTTTTTTTT45930.17778228501910395No Hit
GGTATCAACGCAGAGTACTTTTTTT42860.16589916690439355No Hit
TTGTAGAACAGTGTATATCAATGAG32740.12672745507349148No Hit
GATATACACTGTTCTACAAATCCCG28410.10996722659248298No Hit
CTGAAGGACCTGGAATATGGCGAGA26600.10296121884407064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4650.013.6839218
GGACCGT604.1043994E-412.6627326
CCAACGA3650.012.48951119
CCAACGT1650.012.08738719
TAGGACC7300.011.9723584
CGGTTTC3450.011.56162513
GCCGGTT3650.011.18830411
GTCCTAA15050.010.8418661
GGTATCA14500.010.660841
GGCGAGG11850.010.57919519
CTAGTAC1553.6670826E-910.4191423
GGACCTG16550.010.2717326
AGGACCT17150.010.2476045
GTATCAA28900.010.1694461
TGTAGAA14400.010.0935442
TAGAAAT8500.010.0586194
AATGTCC7400.09.8820658
TGTAGGA28700.09.8308112
AACAGTG9700.09.79077157
TTAGGCT1752.575689E-99.771234
Produced by FastQC (version 0.11.5)