FastQCFastQC Report
Thu 26 May 2016
SRR1511742_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511742_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060741
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57810.28053015881180604No Hit
GGTATCAACGCAGAGTACTTTTTTT33830.16416424965582768No Hit
TATCAACGCAGAGTACTTTTTTTTT33650.1632907774436477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13500.011.4948181
CACGACT855.578049E-511.12554554
CGATCCT700.001549674710.807672510
CTATACT2900.010.7610884
AGTCCGC750.002750421910.08716110
TCTATAC2550.010.0129913
GTCCTAC4550.09.9926961
GTATCAA25750.09.9800411
GTAGGAC4650.09.9651393
GTCAACG800.00325507539.8840792
TAATCCC2400.09.8507425
GTATAAA3050.09.7220451
TAGAACA4400.09.6716394
CGTGCGG800.00468984679.45671410
CCTAAAC1901.1423253E-99.4567143
TAGGACC2500.09.4567144
CGCCCGG800.00468984679.45671410
GTGTAGC4200.09.4134081
AAGGCGT1554.551748E-79.1516596
GTGCGCC1451.8155606E-69.1306211
Produced by FastQC (version 0.11.5)