FastQCFastQC Report
Thu 26 May 2016
SRR1511742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2060741
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76490.3711771639424848No Hit
TATCAACGCAGAGTACTTTTTTTTT47000.22807329984699679No Hit
GGTATCAACGCAGAGTACTTTTTTT46540.22584109308253683No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22780.11054276107477844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15100.012.5048271
CGCCCTA658.043793E-411.68911216
ACACCGT1001.9367508E-611.3968846
GTCCTAA2550.011.2194221
ACCGATC700.001495164610.85417658
TGTACCG803.7720203E-410.6874325
AAGGCGT1409.730138E-810.175796
GTCCTAG2250.010.1722761
ACAGTCG750.002654209510.1305648
ATACCGT856.6114805E-410.0560746
ACCGTGC1356.468308E-79.8491598
GTATAGG2159.094947E-129.7582881
TCTACCC1951.6370905E-109.7435273
TAGACAG3750.09.6266045
TAGGACT2106.730261E-119.499944
TAGGACC2700.09.499944
CTAGACA2201.6370905E-119.4999394
ATTAGCC800.0045167419.4999393
CTAACAC1701.6651029E-89.4999393
CTTATAC1701.6651029E-89.4999393
Produced by FastQC (version 0.11.5)