FastQCFastQC Report
Thu 26 May 2016
SRR1511741_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511741_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533733
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50960.2011261644380051No Hit
GGTATCAACGCAGAGTACTTTTTTT30510.12041521344198461No Hit
TATCAACGCAGAGTACTTTTTTTTT29840.11777089377610031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC6300.010.8264731
TAGGACC4300.010.7761984
GGTATCA14350.010.19502451
TGGACCG1304.092908E-710.18415
TGATCGA750.002750912210.08710910
CGGGACT1054.31851E-59.9069814
GTATTAT2500.09.885041
GCGTAGG907.74086E-49.885041
GTAATAC1752.7866918E-99.7268543
GTATCAA26000.09.6949431
TTAGGAC5100.09.6420893
GTAGGAC7600.09.5810953
TAGGACA4050.09.5734134
TCCTACA7450.09.4206432
CGGTAGG950.00126994239.3647761
CCTATAC2252.910383E-119.2465153
GTCCTAG3750.09.2260371
TGTACTG5450.09.1963895
CTAGGAC3400.09.1785273
GTGTAAG2700.09.1528161
Produced by FastQC (version 0.11.5)