FastQCFastQC Report
Thu 26 May 2016
SRR1511741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533733
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67690.26715522116971285No Hit
GGTATCAACGCAGAGTACTTTTTTT41830.1650923755581192No Hit
TATCAACGCAGAGTACTTTTTTTTT41640.16434249386182365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG4450.011.7428184
GGTATCA14200.011.4208941
AGGACGT5250.011.0392655
CCGGTTT1158.860334E-710.73560512
GTAGGAC7200.010.5566743
GTCTAAC1451.35351E-810.5266821
GTCCTAA4650.010.4630111
GGACGTG5450.010.4552836
TAGGACT3000.010.4511074
GTATCAA30150.010.3466541
ATAGGAC2500.010.2610873
TAGGACC4400.010.1488034
TTAGGAC3850.09.8711763
GTATTAC1752.395609E-99.8123711
CGCCCTA1554.0869054E-89.80323316
GTACTAG1106.5334054E-59.5398041
GTCTTAA2600.09.5398041
CCTATAC3000.09.5010073
CTTACAC3200.09.5010073
GTGCGCC1502.6624184E-79.49688211
Produced by FastQC (version 0.11.5)