FastQCFastQC Report
Thu 26 May 2016
SRR1511740_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511740_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731795
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55240.20221136651908359No Hit
TATCAACGCAGAGTACTTTTTTTTT34290.12552186382946012No Hit
GGTATCAACGCAGAGTACTTTTTTT32630.11944527316288375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGA751.5504176E-512.60914610
GTACTAG2150.011.9513561
CGAACGG907.85416E-611.55838314
GGTATCA14650.011.3332371
TAGGACC3200.010.6389674
TAGGACG13650.010.5307154
CCTATAC2700.010.5076213
TCCAACG2650.010.34920618
GGACGTG13250.010.2776436
GTAGGAC12700.010.2759573
GTCCTAC11050.010.1958831
TGTAGGA13700.010.1714022
AGGACGT13600.010.1522175
CTTAGAC2350.010.0604883
AGTACCG856.878139E-410.0131455
GCCGGTT1901.09139364E-109.95458811
AGGCGTG2201.8189894E-129.8867167
GCGAGCG1151.0841179E-59.8680279
ATAGGAC2600.09.8205843
TAGGACT3800.09.7057244
Produced by FastQC (version 0.11.5)