FastQCFastQC Report
Thu 26 May 2016
SRR1511740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731795
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73500.26905386385142366No Hit
TATCAACGCAGAGTACTTTTTTTTT47430.1736221056118779No Hit
GGTATCAACGCAGAGTACTTTTTTT44020.1611394705678867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAAAT701.09451175E-412.21066212
GTCTTAC1850.011.3433591
GGTATCA16000.010.7310761
GGCGAGG5950.010.37545219
GTATCAA33700.010.331271
AAACCGT856.614369E-410.055846
CTACACT4850.09.9898354
GACGAAA1151.0306803E-59.91010211
AGGACGT11500.09.8305775
TGGCGAG11400.09.83041618
TTAGGAC8150.09.7915523
TATGGCG11800.09.57766616
TAGGACG12400.09.5001374
TCCAACG3200.09.49718218
CGCTCAC900.00111710979.49718110
GACGTGA6850.09.4278597
TAGGACC4850.09.4021984
ATGGCGA12350.09.30493117
CGTGAAA7500.09.2439229
TACACTG17300.09.2255675
Produced by FastQC (version 0.11.5)