FastQCFastQC Report
Thu 26 May 2016
SRR1511739_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511739_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757540
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86980.49489627547594933No Hit
GGTATCAACGCAGAGTACTTTTTTT52410.29820089443199016No Hit
TATCAACGCAGAGTACTTTTTTTTT51440.2926818166300625No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23160.13177509473468596No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19080.10856082934101073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14750.013.26932051
GCCGGTT658.3379657E-411.6389811
TATACCG658.3379657E-411.638985
CCCCGTA951.4310457E-510.9498315
TAGCCCC700.00154953610.8076255
GTATCAA32800.010.6384011
TAGGACC2650.010.3488094
CTCGAAC1106.3843345E-610.31636918
TCGAACT1106.3843345E-610.31636919
CCCGTAC856.876795E-410.01294616
GGCTTAC907.738729E-49.8849741
ATAGACC1356.8573354E-79.8069183
ATTATAC2253.6379788E-129.666823
CGGTCAG2600.09.45667216
TCGGTCA2401.8189894E-129.45667215
TGCCGGT800.0046894399.45667210
ACTATCG2500.09.45667111
GAATTAG1053.0176368E-49.4142611
TTAGGAC4150.09.3427353
GTCCTAG2457.2759576E-129.2797711
Produced by FastQC (version 0.11.5)