##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511739_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1757540 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.251750173538014 31.0 30.0 34.0 23.0 34.0 2 26.578613289028983 31.0 25.0 34.0 10.0 34.0 3 28.247381567418095 31.0 27.0 34.0 16.0 34.0 4 32.750722031931 35.0 32.0 37.0 26.0 37.0 5 33.20607383046759 35.0 35.0 37.0 27.0 37.0 6 33.45910704735027 37.0 35.0 37.0 28.0 37.0 7 33.50759925805387 37.0 35.0 37.0 28.0 37.0 8 33.529605585079146 37.0 35.0 37.0 28.0 37.0 9 35.09981792733024 39.0 35.0 39.0 28.0 39.0 10 34.98781990737053 39.0 35.0 39.0 28.0 39.0 11 34.99962106125607 39.0 35.0 39.0 28.0 39.0 12 34.91381647074889 39.0 35.0 39.0 27.0 39.0 13 34.89015328242885 39.0 35.0 39.0 27.0 39.0 14 36.022813705520214 40.0 36.0 41.0 27.0 41.0 15 35.92679825210237 40.0 36.0 41.0 27.0 41.0 16 35.880130181958876 40.0 36.0 41.0 27.0 41.0 17 35.86560078291248 40.0 36.0 41.0 27.0 41.0 18 35.808030542690354 40.0 36.0 41.0 27.0 41.0 19 35.85244489456854 40.0 36.0 41.0 27.0 41.0 20 35.77892565745303 40.0 36.0 41.0 27.0 41.0 21 35.70033512750776 40.0 36.0 41.0 27.0 41.0 22 35.59282406090331 40.0 36.0 41.0 27.0 41.0 23 35.47898824493326 40.0 36.0 41.0 27.0 41.0 24 35.353573176143925 40.0 36.0 41.0 26.0 41.0 25 35.24007134972746 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7741543546963712 1101 2 3.477996787437661 1101 3 1.9102804903862705 1101 4 0.8509188143107949 1101 5 0.4823700998134939 1101 6 0.20608462702409724 1101 7 0.14392150899244882 1101 8 -0.19855160085114676 1101 9 -0.002676476062731581 1101 10 0.27960712076655625 1101 11 0.20837250724646594 1101 12 -3.169784051024749E-4 1101 13 0.20524181769560812 1101 14 0.12992196936867373 1101 15 0.1650533608096154 1101 16 0.1569469810146913 1101 17 0.29174469316241414 1101 18 0.11329714324698159 1101 19 0.134455178605009 1101 20 0.3938256091024357 1101 21 0.3800600267407148 1101 22 0.3848781196789943 1101 23 0.22166602058973695 1101 24 0.15239665634157262 1101 25 0.1412853730923942 1102 1 1.45999903787013 1102 2 4.132591277561911 1102 3 2.7751118555706427 1102 4 1.6663921628880516 1102 5 1.231794481326986 1102 6 0.9362786296486902 1102 7 0.9166896580734445 1102 8 0.9792061831257826 1102 9 0.9212496612084919 1102 10 1.0894681339433419 1102 11 1.0681788488015798 1102 12 1.1186637378240292 1102 13 1.0038225618121217 1102 14 1.1192634300496707 1102 15 1.10057129389579 1102 16 0.9812485389132135 1102 17 1.0158206809802195 1102 18 1.0825477757874964 1102 19 1.0252315156328322 1102 20 0.9928765556407413 1102 21 1.1794562119941148 1102 22 0.9538098123635876 1102 23 1.0433928495738982 1102 24 0.9512478904022998 1102 25 0.7729071919673913 1103 1 1.566829802660024 1103 2 4.116151256953145 1103 3 2.8649236076008897 1103 4 1.6482906865419764 1103 5 1.1495406746633705 1103 6 1.0473972928313344 1103 7 0.9953629437112497 1103 8 0.8478152425907979 1103 9 0.8539665776809002 1103 10 0.6247027432411159 1103 11 0.9038550025483261 1103 12 1.015779349969776 1103 13 1.046041998159069 1103 14 1.2409147156639833 1103 15 0.9543271068930679 1103 16 0.923253664252428 1103 17 1.2122013926538742 1103 18 1.3172980455642715 1103 19 1.325245109361596 1103 20 1.2116818850537072 1103 21 1.1786481403400586 1103 22 1.4394541634979916 1103 23 1.4125297149271745 1103 24 1.3907876839454474 1103 25 1.1251718903963877 1104 1 1.889041415293569 1104 2 4.409323707441068 1104 3 2.885042738125154 1104 4 2.082305426690816 1104 5 1.5741096900377016 1104 6 1.493628216511695 1104 7 1.2501458340344271 1104 8 1.2257722665772448 1104 9 1.2330312267707484 1104 10 1.2204802067197988 1104 11 1.378380422883481 1104 12 1.3452800691084406 1104 13 1.1950827876400965 1104 14 1.4591499661420144 1104 15 1.5495314132692712 1104 16 1.256519989657292 1104 17 1.1986757625200894 1104 18 1.2898598942811077 1104 19 1.3614401598688204 1104 20 1.4456799529917745 1104 21 1.5649117543169524 1104 22 1.6461612582815022 1104 23 1.7063790252640274 1104 24 1.5940027881706982 1104 25 1.3406642032308795 1105 1 1.4453843374421353 1105 2 3.8417343718284584 1105 3 2.347164025539616 1105 4 1.5238688773187476 1105 5 1.0463229716693618 1105 6 0.7144766458516827 1105 7 0.8190856872626355 1105 8 0.7059097571450224 1105 9 0.7687945234251501 1105 10 0.4356329914313122 1105 11 0.5283283975657653 1105 12 0.6627121055422123 1105 13 0.6869892383972385 1105 14 0.5331482398647509 1105 15 0.7974937868469851 1105 16 0.585957604000221 1105 17 0.8125771873486656 1105 18 0.8806633777220725 1105 19 0.7434910047737446 1105 20 0.8165761263595499 1105 21 0.8376050755811306 1105 22 0.8305502990967213 1105 23 0.9564847196089801 1105 24 0.9105747440397707 1105 25 0.9200698990284124 1106 1 1.5005472540262268 1106 2 4.101838245132289 1106 3 2.576890996618914 1106 4 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1107 17 0.8395252316019182 1107 18 0.8932327862236491 1107 19 0.9092227957214121 1107 20 1.2614201153902727 1107 21 1.1470479192138967 1107 22 1.0896340877630735 1107 23 1.0367975137087484 1107 24 1.2504674977272217 1107 25 1.2832001182839718 1108 1 1.5136829910529066 1108 2 4.078607076846819 1108 3 2.691625469848063 1108 4 1.6672287304399234 1108 5 1.1924674025139197 1108 6 1.04241422235107 1108 7 0.8207074742883336 1108 8 0.8769317889687684 1108 9 0.9383171672440014 1108 10 0.8018508616883508 1108 11 0.8149869042977116 1108 12 0.8326019464234875 1108 13 0.9384274268917281 1108 14 1.0313428543078373 1108 15 1.1359882788910483 1108 16 1.0337544216256802 1108 17 1.1041316549129263 1108 18 0.9693414682973511 1108 19 1.0096476755203767 1108 20 1.085853972136782 1108 21 0.9810873277231096 1108 22 1.06708640680786 1108 23 1.1685716229137526 1108 24 1.2413274980177391 1108 25 1.4042498255889981 1109 1 1.1143278891195294 1109 2 2.823907546701122 1109 3 2.0215628884132286 1109 4 1.1737879360307986 1109 5 0.8611056146985447 1109 6 0.5322797808459541 1109 7 0.5310794751832972 1109 8 0.4611380378994241 1109 9 0.4921003498817669 1109 10 0.30792343246262277 1109 11 0.36231979015075666 1109 12 0.41832864125508706 1109 13 0.3312950516926634 1109 14 0.4795164108548704 1109 15 0.47047782232954205 1109 16 0.43948677576684503 1109 17 0.639777408456581 1109 18 0.3887246035548486 1109 19 0.5340833897120802 1109 20 0.5497660027760247 1109 21 0.45770088900816575 1109 22 0.6094687129106973 1109 23 0.5394923136127403 1109 24 0.4249994028360504 1109 25 0.4839016464389516 1110 1 0.9995018054924891 1110 2 3.560077258748727 1110 3 2.156737271725923 1110 4 1.2190138508124306 1110 5 0.7865549508036267 1110 6 0.6842471847170728 1110 7 0.5059973404842566 1110 8 0.5516580406637104 1110 9 0.503006194286236 1110 10 0.4905146117842918 1110 11 0.419888789835106 1110 12 0.5249216435094723 1110 13 0.43934397203461373 1110 14 0.5397273555486564 1110 15 0.5163948079160505 1110 16 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-2.0938393671760096 2315 21 -2.1548593483398477 2315 22 -2.1677727556411526 2315 23 -2.013364546390278 2315 24 -2.042183617364408 2315 25 -2.183014466379312 2316 1 -0.4411313341980332 2316 2 1.8299319973309345 2316 3 0.5843420215437121 2316 4 -0.42896999730595553 2316 5 -0.7137782086224362 2316 6 -0.8528922752324704 2316 7 -1.397982888799774 2316 8 -1.3477708009771163 2316 9 -1.4665942293440466 2316 10 -1.4634595600556253 2316 11 -1.3505775552008217 2316 12 -1.7178792795668443 2316 13 -1.4775635268857883 2316 14 -1.8283859655525418 2316 15 -1.8224610581323688 2316 16 -1.9775616178637634 2316 17 -1.7606355804127745 2316 18 -1.8491310006191668 2316 19 -1.8472299319809693 2316 20 -1.6219922833880247 2316 21 -2.016912383128222 2316 22 -1.693766994819633 2316 23 -2.001558287450159 2316 24 -1.8257271097727497 2316 25 -1.8335036958286537 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62962.0 3 1.0 4 564.0 5 2672.0 6 3272.0 7 2949.0 8 2827.0 9 2624.0 10 2527.0 11 2559.0 12 2452.0 13 2455.0 14 2395.0 15 2567.0 16 2754.0 17 2928.0 18 3424.0 19 4245.0 20 5436.0 21 6922.0 22 8819.0 23 10351.0 24 11891.0 25 14040.0 26 15415.0 27 17475.0 28 20540.0 29 24709.0 30 30755.0 31 39314.0 32 50720.0 33 66946.0 34 91234.0 35 129626.0 36 207039.0 37 263579.0 38 636552.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.77285296493963 19.44809221980723 13.953764921424265 23.825289893828874 2 16.51529470399352 21.739813096118173 39.214071139568894 22.53082106031941 3 18.114296118438272 25.86928320265826 30.12904400468837 25.887376674215094 4 12.148059219135837 17.583668081523037 39.21765649714942 31.0506162021917 5 13.416593647939735 37.50366990224974 35.31418915074479 13.765547299065739 6 30.137749354211003 37.95173936297325 18.716046291976284 13.194464990839469 7 26.81002992819509 32.471067514821854 22.94269262719483 17.776209929788227 8 25.092117391353824 35.58860680269012 21.32309933202089 17.99617647393516 9 26.066206174539413 15.891473309284567 21.027629527635217 37.0146909885408 10 15.202840333648169 29.13168405839981 33.789046053005904 21.876429554946117 11 32.99446954265621 23.92895752017024 24.320641350979212 18.75593158619434 12 22.692172013154753 25.621493678664496 31.53953821819134 20.146796089989415 13 27.918738691580273 22.672542303446864 26.450436405430317 22.958282599542542 14 21.44264141925646 22.170420018890038 28.437076823287093 27.949861738566405 15 23.256312800846636 29.35466618113955 24.706180229183975 22.682840788829843 16 22.285524084800347 28.97311014258566 26.752108060129498 21.989257712484495 17 21.3036972131502 28.37266861636151 27.994412644946916 22.32922152554138 18 21.134711016534474 28.080954060789516 29.438362711517236 21.345972211158777 19 22.46294392329274 27.25327859011948 29.027634664152547 21.256142822435233 20 23.082547196649863 26.872162226748753 28.96121852134233 21.084072055259057 21 23.2574507550326 26.877965793097168 28.221093118791035 21.64349033307919 22 22.617772151178503 27.11221746490093 28.836915548854215 21.433094835066356 23 22.40680544831875 27.135991187647683 28.53784253268644 21.91936083134712 24 22.223733172502474 26.795862398579835 28.959682283191277 22.020722145726413 25 22.502645743482365 27.06692308567657 28.644924155353507 21.785507015487557 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 273.0 1 273.0 2 532.5 3 792.0 4 792.0 5 792.0 6 2004.0 7 3216.0 8 3216.0 9 3216.0 10 4480.0 11 5744.0 12 5744.0 13 5744.0 14 8472.5 15 11201.0 16 11201.0 17 11201.0 18 18863.0 19 26525.0 20 26525.0 21 26525.0 22 39517.5 23 52510.0 24 52510.0 25 52510.0 26 72166.5 27 91823.0 28 91823.0 29 91823.0 30 114040.5 31 136258.0 32 136258.0 33 136258.0 34 152262.5 35 168267.0 36 168267.0 37 168267.0 38 181123.5 39 193980.0 40 193980.0 41 193980.0 42 203064.5 43 212149.0 44 212149.0 45 212149.0 46 216310.0 47 220471.0 48 220471.0 49 220471.0 50 213880.0 51 207289.0 52 207289.0 53 207289.0 54 186984.0 55 166679.0 56 166679.0 57 166679.0 58 142673.0 59 118667.0 60 118667.0 61 118667.0 62 96773.5 63 74880.0 64 74880.0 65 74880.0 66 57264.0 67 39648.0 68 39648.0 69 39648.0 70 28517.0 71 17386.0 72 17386.0 73 17386.0 74 11996.5 75 6607.0 76 6607.0 77 6607.0 78 4414.0 79 2221.0 80 2221.0 81 2221.0 82 1447.0 83 673.0 84 673.0 85 673.0 86 436.5 87 200.0 88 200.0 89 200.0 90 127.5 91 55.0 92 55.0 93 55.0 94 38.5 95 22.0 96 22.0 97 22.0 98 13.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.3322484836760475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 5.689770929822365E-5 20 0.0 21 0.0 22 8.534656394733548E-4 23 3.9828396508756556E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1757540.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.32189699134294 #Duplication Level Percentage of deduplicated Percentage of total 1 82.97029592067233 61.6650978675745 2 12.057994843077081 17.923461012986387 3 2.888564734779374 6.440508320132954 4 0.9611366481705556 2.8573399583974664 5 0.39616188147705417 1.4721751273517116 6 0.20635577688302695 0.9202051675841326 7 0.13343079714064734 0.6941780972391978 8 0.07763575127794899 0.4616029047460197 9 0.04836455196890251 0.3235090724518702 >10 0.23413810937617827 3.0531977512156274 >50 0.014083489177773936 0.7224125505484811 >100 0.010213146223278154 1.423534309958836 >500 0.0010828998506593056 0.5514246983543841 >1k 3.0939995733123016E-4 0.39431005066854885 >5k 2.320499679984226E-4 1.097043110789899 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8698 0.49489627547594933 No Hit GGTATCAACGCAGAGTACTTTTTTT 5241 0.29820089443199016 No Hit TATCAACGCAGAGTACTTTTTTTTT 5144 0.2926818166300625 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2316 0.13177509473468596 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1908 0.10856082934101073 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1475 0.0 13.2693205 1 GCCGGTT 65 8.3379657E-4 11.63898 11 TATACCG 65 8.3379657E-4 11.63898 5 CCCCGTA 95 1.4310457E-5 10.94983 15 TAGCCCC 70 0.001549536 10.807625 5 GTATCAA 3280 0.0 10.638401 1 TAGGACC 265 0.0 10.348809 4 CTCGAAC 110 6.3843345E-6 10.316369 18 TCGAACT 110 6.3843345E-6 10.316369 19 CCCGTAC 85 6.876795E-4 10.012946 16 GGCTTAC 90 7.738729E-4 9.884974 1 ATAGACC 135 6.8573354E-7 9.806918 3 ATTATAC 225 3.6379788E-12 9.66682 3 CGGTCAG 260 0.0 9.456672 16 TCGGTCA 240 1.8189894E-12 9.456672 15 TGCCGGT 80 0.004689439 9.456672 10 ACTATCG 250 0.0 9.456671 11 GAATTAG 105 3.0176368E-4 9.414261 1 TTAGGAC 415 0.0 9.342735 3 GTCCTAG 245 7.2759576E-12 9.279771 1 >>END_MODULE