FastQCFastQC Report
Thu 26 May 2016
SRR1511739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511739_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757540
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT112990.642887217360629No Hit
GGTATCAACGCAGAGTACTTTTTTT69740.39680462464581173No Hit
TATCAACGCAGAGTACTTTTTTTTT68430.38935102472774447No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31280.17797603468484358No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23720.1349613664553865No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19250.10952809039908053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG551.9593358E-413.8190515
CACCGTT655.469007E-513.1498097
GGTATCA18500.013.0446831
GCTGCGT550.003075784512.0871978
GTCATAT1251.7254933E-811.4463241
AGCGTGC600.00588961911.07993110
ACCGTGC600.00588961911.0799318
TAGGACG4550.011.066634
CCCCGTA700.001495395410.8538115
CGAACCG803.7819176E-410.68421912
GCCGGTT909.545442E-510.55231611
TCCAACG1201.5296646E-610.28850718
TTAGGAC4350.010.2650133
AGGACGT4750.010.2006425
CCCCCGG750.002654617710.13022318
GTATCAA39800.010.08982851
GGACGTG4950.09.7848746
GTAGGAC5250.09.7720423
TTAGACA2054.1836756E-119.732324
TAGGACT2650.09.6798534
Produced by FastQC (version 0.11.5)