FastQCFastQC Report
Thu 26 May 2016
SRR1511738_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511738_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654970
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57920.21815689066166472No Hit
TATCAACGCAGAGTACTTTTTTTTT35520.1337868224499712No Hit
GGTATCAACGCAGAGTACTTTTTTT33840.12745906733409418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.011.2421421
GCGTAGG802.6082838E-411.122281
GCGTCGG951.4314102E-510.95010418
TGTGCGG700.001550393410.80728410
CGTAGGA1209.1332186E-710.7103432
GGACGTG9350.010.6194576
CCGAGCA909.974622E-510.5070829
AGGACGT10050.010.4443535
CGTCGGT1002.5235822E-510.40259819
GTCCTAC8400.010.239561
ATTATAC3050.09.9214423
TAGGACG10200.09.9199224
AGCGTCG1054.317498E-59.90723717
GTATAAT4100.09.8864711
GGCGAGG4450.09.77568119
GTCCTAA5200.09.6963471
GACGTGG5900.09.6166527
GTCTTAT2900.09.5455581
ATAAGCG1107.1926865E-59.45637414
TAAGCCG900.00116194739.4563745
Produced by FastQC (version 0.11.5)