FastQCFastQC Report
Thu 26 May 2016
SRR1511738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511738_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654970
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74840.2818864243287118No Hit
TATCAACGCAGAGTACTTTTTTTTT47700.17966304704007957No Hit
GGTATCAACGCAGAGTACTTTTTTT45160.17009608394821787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG508.739712E-515.1954969
GGTATCA15900.011.8168021
TCCAACG1800.011.60767118
GGCGCGC700.00149569910.8539258
CTAGACG803.771083E-410.6879564
GTATCAA32500.010.3297591
CCAACGT1409.730684E-810.17612919
AGCGTAC856.3620537E-410.0984431
TTAGGAC7950.010.0381643
GTCCTAG3300.09.8264311
TAGGACC4000.09.5004054
GTAGAAC6100.09.5004043
CGTGCGC1002.764706E-49.49718510
GACGTGA5900.09.4971857
GGCGAGG5600.09.32811819
TGTAGAA8450.09.2193282
TGGCGAG9650.09.0543118
GTCCTAC9500.09.0354491
GTCCTAA6150.08.9946381
TGCCGGT1804.2417014E-88.969563510
Produced by FastQC (version 0.11.5)