FastQCFastQC Report
Thu 26 May 2016
SRR1511737_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511737_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376979
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT60700.25536616015539054No Hit
GTATCAACGCAGAGTACTTTTTTTT56840.2391270600203031No Hit
CTGTAGGACGTGGAATATGGCAAGA55580.23382621386221755No Hit
GTCCTAAAGTGTGTATTTCTCATTT50050.2105613890572866No Hit
CTTTAGGACGTGAAATATGGCGAGG44710.18809589819682881No Hit
GGTATCAACGCAGAGTACTTTTTTT33940.14278628460747866No Hit
TATCAACGCAGAGTACTTTTTTTTT32850.13820063197865862No Hit
GTCCTACAGTGTGCATTTCTCATTT26110.1098453120536614No Hit
CTGAAGGACCTGGAATATGGCGAGA24700.10391341278151804No Hit
TTGTAGAACAGTGTATATCAATGAG24380.10256716613819473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT307.9651095E-418.913484
CGGTAGG1200.015.6496721
TCCAACG4200.014.41057518
TAGGACC9100.013.821394
CCAACGA2900.013.37015619
CGTTAGG903.3430115E-713.178671
AGGACCT17450.012.1393125
GGTATCA12000.012.0255371
CCAACGT1900.011.94560819
GGACCTG16400.011.878596
TAGGACT4850.011.699064
GACCGTG1002.0397401E-611.3480897
CTAGGAC3750.011.3480883
GATATAC9850.011.1383181
TAAGACG600.00608024811.0328644
CGGTTTC3400.010.84743913
TGGACCG700.001549789110.8077045
ACTGTTC10700.010.6940728
CGGACAG803.934705E-410.6388345
CCGGTTT3200.010.63883412
Produced by FastQC (version 0.11.5)