##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511737_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2376979 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.007556229987728 31.0 28.0 34.0 20.0 34.0 2 26.3956728267267 31.0 25.0 34.0 10.0 34.0 3 28.039531691277038 31.0 27.0 34.0 16.0 34.0 4 32.47248292896151 35.0 32.0 37.0 24.0 37.0 5 32.91884530742594 35.0 33.0 37.0 26.0 37.0 6 33.16646634236146 37.0 35.0 37.0 27.0 37.0 7 33.197763211202115 37.0 35.0 37.0 27.0 37.0 8 33.21125386467445 37.0 35.0 37.0 27.0 37.0 9 34.77047630626943 39.0 35.0 39.0 27.0 39.0 10 34.63312002335738 39.0 35.0 39.0 27.0 39.0 11 34.64148358062903 39.0 35.0 39.0 27.0 39.0 12 34.545905958782136 39.0 35.0 39.0 27.0 39.0 13 34.54316424335259 39.0 35.0 39.0 27.0 39.0 14 35.641183199346735 40.0 36.0 41.0 27.0 41.0 15 35.58847553975025 40.0 36.0 41.0 27.0 41.0 16 35.52681702278396 40.0 36.0 41.0 27.0 41.0 17 35.50131280082828 40.0 36.0 41.0 26.0 41.0 18 35.43990838791592 40.0 36.0 41.0 26.0 41.0 19 35.46988719715235 40.0 36.0 41.0 26.0 41.0 20 35.399785610222054 40.0 36.0 41.0 25.0 41.0 21 35.30463710449272 40.0 36.0 41.0 25.0 41.0 22 35.19546281225034 40.0 36.0 41.0 25.0 41.0 23 35.088335235607886 40.0 36.0 41.0 24.0 41.0 24 34.980102895313756 40.0 36.0 41.0 24.0 41.0 25 34.86790165163428 40.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3155058524454972 1101 2 3.812850836109824 1101 3 2.507760609417545 1101 4 1.4319685682703565 1101 5 1.0731789426987746 1101 6 1.0799591776283606 1101 7 0.965149013349766 1101 8 0.6822471695358558 1101 9 0.6514445275656513 1101 10 0.9007037320963818 1101 11 0.8029724965004377 1101 12 0.9180089038136003 1101 13 0.9127166958512447 1101 14 1.1568774391213807 1101 15 0.9177242063077742 1101 16 1.1408035313104108 1101 17 1.0421069735637758 1101 18 1.0954426051631998 1101 19 1.102391313600208 1101 20 1.176964143957342 1101 21 1.0406500030562995 1101 22 1.0977384083991382 1101 23 1.1492526538479453 1101 24 1.0851155911573542 1101 25 0.9605371700238052 1102 1 1.6760999039527995 1102 2 4.274901742743456 1102 3 2.8868047960752 1102 4 1.8641735369229835 1102 5 1.4308224460963004 1102 6 1.1380156404432427 1102 7 1.0215210240867094 1102 8 1.011231524005204 1102 9 1.2743872388319346 1102 10 1.1472214987367693 1102 11 1.148417507199838 1102 12 1.1357809408297754 1102 13 1.2720021822048082 1102 14 1.3352123210525235 1102 15 1.415917039584798 1102 16 1.4453080738640054 1102 17 1.3838759415406514 1102 18 1.4044683494286971 1102 19 1.3821199335649155 1102 20 1.376397028666581 1102 21 1.4968741930927152 1102 22 1.2994960669192466 1102 23 1.2436446180331018 1102 24 1.2095008447519788 1102 25 1.2235099616294676 1103 1 1.2024713231762973 1103 2 3.7612838861199265 1103 3 2.4279782728819477 1103 4 1.356736609483697 1103 5 1.0033758850958634 1103 6 0.7183631757057114 1103 7 0.6245337840110992 1103 8 0.39142546758525043 1103 9 0.6263103157550134 1103 10 0.5270028350752938 1103 11 0.7383377122311003 1103 12 0.8173581183781309 1103 13 0.704198270615457 1103 14 1.0005378627131307 1103 15 0.713704955827346 1103 16 0.6543981567489823 1103 17 0.8745041224334855 1103 18 0.8043856462150885 1103 19 0.8552722892432669 1103 20 0.9203778706034669 1103 21 0.9539130545148424 1103 22 1.0670954925408793 1103 23 0.8084157755145611 1103 24 1.074449223405459 1103 25 0.6567212092665144 1104 1 1.4560786262109353 1104 2 4.007154130878195 1104 3 2.654462628438914 1104 4 1.7284013434301997 1104 5 1.369488681821359 1104 6 1.0786857546427626 1104 7 1.0794166823500149 1104 8 1.055665643806968 1104 9 1.1477855725243913 1104 10 1.1179170205352307 1104 11 1.1599178124501393 1104 12 1.1219759957748323 1104 13 1.0718863666478171 1104 14 1.3080490839628212 1104 15 1.256530592698347 1104 16 1.0993041594777395 1104 17 1.2842350595468304 1104 18 1.1460124670757565 1104 19 1.2604242681192517 1104 20 1.3247051339599807 1104 21 1.216578549194132 1104 22 1.2517849953111124 1104 23 1.3173226148728645 1104 24 1.284538552142621 1104 25 1.184136712329746 1105 1 1.4246961278564 1105 2 3.867917015266922 1105 3 2.3196407215340784 1105 4 1.575954572372055 1105 5 1.056918845143521 1105 6 0.7750550127285152 1105 7 0.774924940298007 1105 8 0.6951629727932058 1105 9 0.7233048512741362 1105 10 0.4872286796250975 1105 11 0.5858220539281547 1105 12 0.7419994149785794 1105 13 0.6076771857076793 1105 14 0.5686183389872355 1105 15 0.7389011365442997 1105 16 0.7647577726169814 1105 17 0.8740048472433273 1105 18 0.7399533221062953 1105 19 0.6865887229722318 1105 20 0.6787062972668281 1105 21 0.7080527344966683 1105 22 0.7492517415724578 1105 23 0.9000207214378904 1105 24 0.6776597350420133 1105 25 0.7103838231959472 1106 1 1.5813908449612484 1106 2 3.8796087685696143 1106 3 2.4320263615133726 1106 4 1.6158761889640871 1106 5 1.091747982653608 1106 6 0.9692549787222902 1106 7 0.9602426589500439 1106 8 0.8013704239139585 1106 9 0.7895387840376316 1106 10 0.7747780802419086 1106 11 0.999468554527958 1106 12 0.8865453614272312 1106 13 1.0915498277001063 1106 14 1.0898987672777807 1106 15 1.1782256245016853 1106 16 0.9770071058789185 1106 17 1.0529083531723415 1106 18 1.1110793602590263 1106 19 1.16255022078672 1106 20 1.051488025215157 1106 21 1.0119838352556059 1106 22 0.8869854051982955 1106 23 0.8312490037196199 1106 24 0.7799475460651024 1106 25 0.7323321637955331 1107 1 1.6877983313187244 1107 2 4.116309790724486 1107 3 2.6399465772314876 1107 4 1.7501309781671424 1107 5 1.4880893405798759 1107 6 1.3420291699780762 1107 7 1.0892510784838123 1107 8 1.1384828141928054 1107 9 1.1943066892817598 1107 10 1.2059978255377999 1107 11 1.309999120283038 1107 12 1.2238410391629984 1107 13 1.2334002922543235 1107 14 1.3807249962739476 1107 15 1.4050001346402397 1107 16 1.4564237374388682 1107 17 1.4673266703223007 1107 18 1.3273094645692254 1107 19 1.5542261648223246 1107 20 1.5594079603912476 1107 21 1.602191422327671 1107 22 1.5875021168529955 1107 23 1.7394266635034938 1107 24 1.6998941776203793 1107 25 1.562976190097281 1108 1 1.396451379726603 1108 2 3.8620899365530406 1108 3 2.5346303152080196 1108 4 1.5185396398353532 1108 5 1.0592931265194423 1108 6 0.918060044256741 1108 7 0.7178177133906161 1108 8 0.6592619210275359 1108 9 0.6615395177923631 1108 10 0.5651895345210178 1108 11 0.6009127729697212 1108 12 0.6304043246615691 1108 13 0.6261503472825183 1108 14 0.6628953235339168 1108 15 0.6423944458796882 1108 16 0.7231796293034733 1108 17 0.8620723631156793 1108 18 0.8453533239618025 1108 19 0.6951333526585586 1108 20 0.7828004830220721 1108 21 0.7668469304572199 1108 22 0.8748397067259575 1108 23 0.8349409808383754 1108 24 0.7556677508509679 1108 25 0.9205282129028092 1109 1 1.0382542827488699 1109 2 3.169068117914378 1109 3 1.898583344108399 1109 4 1.2496210756389203 1109 5 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-2.5962473754103 2315 20 -2.5375061503758616 2315 21 -2.6354130368554536 2315 22 -2.56487024138886 2315 23 -2.663319319463689 2315 24 -2.373593012691451 2315 25 -2.4136773298982206 2316 1 -1.0305242908179473 2316 2 1.326581672009592 2316 3 -0.12138012245454632 2316 4 -1.0082836754049183 2316 5 -1.3359010602823744 2316 6 -1.6126790671480187 2316 7 -1.8453201089182159 2316 8 -1.8050317385942662 2316 9 -2.0671215397458056 2316 10 -2.1165013445119314 2316 11 -2.085511853669182 2316 12 -2.25191562984093 2316 13 -2.186541142286792 2316 14 -2.3202261341704116 2316 15 -2.5590687504871994 2316 16 -2.542071931188417 2316 17 -2.482550735230852 2316 18 -2.6523358377634167 2316 19 -2.452657286341612 2316 20 -2.384483828981523 2316 21 -2.5956916462403683 2316 22 -2.404965061033259 2316 23 -2.6966507905812733 2316 24 -2.732200557294256 2316 25 -2.555523289602064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99352.0 3 5.0 4 906.0 5 4205.0 6 5113.0 7 4538.0 8 4051.0 9 3992.0 10 3974.0 11 3747.0 12 3603.0 13 3521.0 14 3724.0 15 3734.0 16 4050.0 17 4315.0 18 5009.0 19 6189.0 20 7860.0 21 10096.0 22 12307.0 23 15009.0 24 17267.0 25 19490.0 26 21986.0 27 25047.0 28 28760.0 29 34689.0 30 43000.0 31 54124.0 32 69224.0 33 89964.0 34 122662.0 35 173643.0 36 274840.0 37 348324.0 38 844659.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.21539189029436 19.390873878145324 13.42153212123456 24.972202110325753 2 15.6196698359821 21.204263647528386 41.52074375066782 21.655322765821698 3 18.759568342841902 24.919572280613334 29.8670286948265 26.453830681718266 4 11.670654221177386 17.391613472395 39.75966973204223 31.178062574385386 5 12.578318950230525 38.22469613740803 36.60558212756613 12.591402784795322 6 30.46156486868416 38.33883261063728 18.8108939961186 12.388708524559956 7 27.03688168890007 31.740372969218487 23.05253853736192 18.170206804519516 8 23.61110468371828 39.00964207088073 21.17157955539363 16.207673690007358 9 25.845789971219773 15.810068157943338 20.941034817724518 37.40310705311238 10 14.248842753764338 29.928324987305317 35.44330850209447 20.379523756835884 11 33.69874113317787 22.69893844245153 25.60304487334554 17.99927555102506 12 22.948667194788005 24.725376202313946 33.19436141421527 19.13159518868278 13 28.82255165064563 22.55000990753389 26.260433937363352 22.367004504457128 14 19.9037097088363 23.176687720000892 28.485611357946368 28.43399121321644 15 22.069904698358712 32.50975292587776 23.821750213190775 21.598592162572743 16 20.831105365255645 28.835887906456055 29.489658932619932 20.843347795668368 17 20.666779134354996 29.33269498804996 29.065885731426317 20.93464014616873 18 20.33896807670577 28.20058570143026 31.579033723057716 19.88141249880626 19 22.83385759823709 27.245087146331542 28.19608418921665 21.72497106621472 20 22.531667297018608 28.376944011705614 29.264667462354527 19.826721228921247 21 22.039880133261928 27.50102756741452 27.885297813647863 22.573794485675688 22 21.9755485998923 29.317910271943997 28.403717353257946 20.30282377490576 23 21.514713727258805 28.606323023823666 28.668671464944357 21.21029178397317 24 22.339238167438584 27.489767473755556 29.657014218468063 20.513980140337797 25 22.001372330172035 28.145642010299625 29.114939593492412 20.738046066035924 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 218.0 1 218.0 2 408.5 3 599.0 4 599.0 5 599.0 6 1676.0 7 2753.0 8 2753.0 9 2753.0 10 4032.0 11 5311.0 12 5311.0 13 5311.0 14 9058.0 15 12805.0 16 12805.0 17 12805.0 18 22623.0 19 32441.0 20 32441.0 21 32441.0 22 51796.0 23 71151.0 24 71151.0 25 71151.0 26 102881.5 27 134612.0 28 134612.0 29 134612.0 30 175812.5 31 217013.0 32 217013.0 33 217013.0 34 238946.5 35 260880.0 36 260880.0 37 260880.0 38 273404.0 39 285928.0 40 285928.0 41 285928.0 42 296170.5 43 306413.0 44 306413.0 45 306413.0 46 305014.5 47 303616.0 48 303616.0 49 303616.0 50 284763.5 51 265911.0 52 265911.0 53 265911.0 54 230055.5 55 194200.0 56 194200.0 57 194200.0 58 163233.0 59 132266.0 60 132266.0 61 132266.0 62 106075.5 63 79885.0 64 79885.0 65 79885.0 66 60602.0 67 41319.0 68 41319.0 69 41319.0 70 30042.0 71 18765.0 72 18765.0 73 18765.0 74 12995.0 75 7225.0 76 7225.0 77 7225.0 78 4885.0 79 2545.0 80 2545.0 81 2545.0 82 1654.0 83 763.0 84 763.0 85 763.0 86 510.0 87 257.0 88 257.0 89 257.0 90 166.5 91 76.0 92 76.0 93 76.0 94 49.0 95 22.0 96 22.0 97 22.0 98 13.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.323807656693644 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 2.5242124562312076E-4 22 7.993339444732157E-4 23 6.31053114057802E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2376979.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.08196272665155 #Duplication Level Percentage of deduplicated Percentage of total 1 87.88959802850799 66.86813121265332 2 8.974134389831251 13.655395163022064 3 1.7323804441687247 3.954087131448749 4 0.5319399183886117 1.6188413217464166 5 0.24644310905906744 0.9374937718836046 6 0.14694505607853098 0.670792096765951 7 0.09047408359889675 0.4818412099269453 8 0.062396030345070616 0.37977699640037454 9 0.0431774845655322 0.2956524994210854 >10 0.24263410109492672 3.327769916033301 >50 0.01947307796911663 1.0290031605532686 >100 0.016541892459876296 2.495832581812492 >500 0.0020711334130422508 1.1410624211609222 >1k 0.0015673442044603358 2.193882341043363 >5k 2.2390631492290512E-4 0.9504381761281347 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6070 0.25536616015539054 No Hit GTATCAACGCAGAGTACTTTTTTTT 5684 0.2391270600203031 No Hit CTGTAGGACGTGGAATATGGCAAGA 5558 0.23382621386221755 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5005 0.2105613890572866 No Hit CTTTAGGACGTGAAATATGGCGAGG 4471 0.18809589819682881 No Hit GGTATCAACGCAGAGTACTTTTTTT 3394 0.14278628460747866 No Hit TATCAACGCAGAGTACTTTTTTTTT 3285 0.13820063197865862 No Hit GTCCTACAGTGTGCATTTCTCATTT 2611 0.1098453120536614 No Hit CTGAAGGACCTGGAATATGGCGAGA 2470 0.10391341278151804 No Hit TTGTAGAACAGTGTATATCAATGAG 2438 0.10256716613819473 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.207020760385346E-5 2 0.0 0.0 0.0 0.0 4.207020760385346E-5 3 0.0 0.0 0.0 0.0 4.207020760385346E-5 4 0.0 0.0 0.0 0.0 4.207020760385346E-5 5 0.0 0.0 0.0 0.0 4.207020760385346E-5 6 0.0 0.0 0.0 0.0 4.207020760385346E-5 7 0.0 0.0 0.0 8.414041520770692E-5 4.207020760385346E-5 8 0.0 0.0 0.0 8.414041520770692E-5 4.207020760385346E-5 9 0.0 0.0 0.0 1.2621062281156038E-4 4.207020760385346E-5 10 0.0 0.0 0.0 1.2621062281156038E-4 4.207020760385346E-5 11 0.0 0.0 0.0 1.2621062281156038E-4 8.414041520770692E-5 12 0.0 0.0 0.0 1.2621062281156038E-4 1.2621062281156038E-4 13 0.0 0.0 0.0 1.2621062281156038E-4 1.2621062281156038E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGCT 30 7.9651095E-4 18.91348 4 CGGTAGG 120 0.0 15.649672 1 TCCAACG 420 0.0 14.410575 18 TAGGACC 910 0.0 13.82139 4 CCAACGA 290 0.0 13.370156 19 CGTTAGG 90 3.3430115E-7 13.17867 1 AGGACCT 1745 0.0 12.139312 5 GGTATCA 1200 0.0 12.025537 1 CCAACGT 190 0.0 11.945608 19 GGACCTG 1640 0.0 11.87859 6 TAGGACT 485 0.0 11.69906 4 GACCGTG 100 2.0397401E-6 11.348089 7 CTAGGAC 375 0.0 11.348088 3 GATATAC 985 0.0 11.138318 1 TAAGACG 60 0.006080248 11.032864 4 CGGTTTC 340 0.0 10.847439 13 TGGACCG 70 0.0015497891 10.807704 5 ACTGTTC 1070 0.0 10.694072 8 CGGACAG 80 3.934705E-4 10.638834 5 CCGGTTT 320 0.0 10.638834 12 >>END_MODULE