FastQCFastQC Report
Thu 26 May 2016
SRR1511737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511737_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376979
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74460.3132547658182929No Hit
GTCCTACAGTGGACATTTCTAAATT66210.2785468445451138No Hit
CTGTAGGACGTGGAATATGGCAAGA61890.2603725148602491No Hit
GTCCTAAAGTGTGTATTTCTCATTT54790.2305026674615131No Hit
CTTTAGGACGTGAAATATGGCGAGG49340.20757440431741297No Hit
TATCAACGCAGAGTACTTTTTTTTT43980.18502477304174753No Hit
GGTATCAACGCAGAGTACTTTTTTT43870.18456200075810514No Hit
GTCCTACAGTGTGCATTTCTCATTT28720.12082563623826714No Hit
TTGTAGAACAGTGTATATCAATGAG28080.11813314295162053No Hit
CTGAAGGACCTGGAATATGGCGAGA27570.11598756236382399No Hit
GATATACACTGTTCTACAAATCCCG24640.10366099153589495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2300.014.86487319
TCCAACG3700.014.63020918
GCCGGTT2400.012.26670411
TAGGACC8100.012.0815194
CGGTTTC2450.012.01636213
CGTTTCC2650.011.82620714
GGTATCA13300.011.6210691
CCAACGT1800.011.60744819
CCGGTTT2300.011.56132412
GGACCGC752.0811931E-411.3961626
GCGCCAC951.3660099E-510.99629813
GGCGAGG13050.010.91609519
GTATCAA27550.010.5969911
TGCCGGT2700.010.55200310
CCTACAC3450.010.4648693
GTCCTAC31600.010.2955611
AGGACCT17450.010.1815865
TGTAGGA32300.010.1187122
ATCCCGT3100.010.109510
GTCTAAG1701.4479156E-910.1019751
Produced by FastQC (version 0.11.5)