FastQCFastQC Report
Thu 26 May 2016
SRR1511736_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511736_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916836
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66270.3457259776005876No Hit
GGTATCAACGCAGAGTACTTTTTTT40470.2111291732834734No Hit
TATCAACGCAGAGTACTTTTTTTTT40020.2087815546035237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA551.400295E-414.3767181
ACCGTCC602.6817252E-514.1851658
GGTATCA12600.013.8846581
CCCTTAT1051.6258127E-712.2373261
TTACCCG550.00317549412.0358974
ACCGTGC951.0990625E-611.9454028
GGACCGG658.3377893E-411.639116
CGTGCGC752.1640143E-411.34813210
TAGGACC4450.010.8381034
TGTCGAA700.00154950210.80774614
TATGACT1855.456968E-1210.734724
GTAATAC1502.2992026E-910.717683
ACCGCAT803.9338192E-410.6388748
GTAGGAC8100.010.507533
TCGAAAC909.966999E-510.5075316
GTATCAA26400.010.4830241
ATAAGAC1651.0059011E-910.31648353
TAGACTG2250.010.0872295
GTCGAAA856.876695E-410.01305815
CTAGCAC1701.6862032E-910.0130583
Produced by FastQC (version 0.11.5)