##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511736_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1916836 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.094390965111256 31.0 28.0 34.0 22.0 34.0 2 26.447384126758887 31.0 25.0 34.0 10.0 34.0 3 28.100852133411518 31.0 27.0 34.0 16.0 34.0 4 32.57873704375335 35.0 32.0 37.0 25.0 37.0 5 33.01928803507447 35.0 35.0 37.0 27.0 37.0 6 33.26288738316684 37.0 35.0 37.0 27.0 37.0 7 33.30183542045329 37.0 35.0 37.0 27.0 37.0 8 33.32592198810957 37.0 35.0 37.0 27.0 37.0 9 34.88549724650414 39.0 35.0 39.0 28.0 39.0 10 34.76257541072893 39.0 35.0 39.0 27.0 39.0 11 34.77405735284604 39.0 35.0 39.0 27.0 39.0 12 34.66870196511334 39.0 35.0 39.0 27.0 39.0 13 34.65147826939811 39.0 35.0 39.0 27.0 39.0 14 35.76422500412138 40.0 36.0 41.0 27.0 41.0 15 35.682657253933044 40.0 36.0 41.0 27.0 41.0 16 35.617254162588765 40.0 36.0 41.0 27.0 41.0 17 35.58628281188375 40.0 36.0 41.0 27.0 41.0 18 35.52057922534844 40.0 36.0 41.0 27.0 41.0 19 35.544717440615685 40.0 36.0 41.0 26.0 41.0 20 35.47332166132105 40.0 36.0 41.0 26.0 41.0 21 35.38894563749846 40.0 36.0 41.0 26.0 41.0 22 35.277189076165094 40.0 36.0 41.0 25.0 41.0 23 35.14745184251548 40.0 36.0 41.0 25.0 41.0 24 35.022254381699845 40.0 35.0 41.0 24.0 41.0 25 34.89331220824317 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.173312941986321 1101 2 3.6542089253251717 1101 3 2.2277857627688213 1101 4 1.3560244179860916 1101 5 0.967735001498994 1101 6 0.726052517459415 1101 7 0.6009997869345156 1101 8 0.2919689715149545 1101 9 0.3635556757476479 1101 10 0.6377669591391282 1101 11 0.5183844540611915 1101 12 0.6637180257782589 1101 13 0.6407224590395728 1101 14 0.7811268891213103 1101 15 0.7863333240747892 1101 16 0.6964733554335325 1101 17 0.7257921831834366 1101 18 0.6723238399603559 1101 19 0.8206706049177654 1101 20 0.7974223143128469 1101 21 0.8210794716334036 1101 22 0.6857126290055149 1101 23 0.6077619208285086 1101 24 0.707957525329391 1101 25 0.5973330760844533 1102 1 1.9215659103331397 1102 2 4.562810473548755 1102 3 3.102210688617543 1102 4 2.1207219817837384 1102 5 1.480244758246073 1102 6 1.3074738344595715 1102 7 1.1678516404868944 1102 8 1.3198388325788812 1102 9 1.4938874510623918 1102 10 1.4293940631529338 1102 11 1.482255563988737 1102 12 1.408619264540782 1102 13 1.5061298548834259 1102 14 1.682365651644382 1102 15 1.7213556842433917 1102 16 1.6137196111383574 1102 17 1.59110040456288 1102 18 1.625928556257108 1102 19 1.6729998274083329 1102 20 1.7899456616346754 1102 21 1.80050811652454 1102 22 1.4111095564336793 1102 23 1.5887332796104943 1102 24 1.4462012190272233 1102 25 1.428258979903589 1103 1 1.5009958023398084 1103 2 3.937167361783814 1103 3 2.5419769222471444 1103 4 1.6413876513371264 1103 5 1.254901832519323 1103 6 0.8602183799985994 1103 7 0.8424917065558759 1103 8 0.721668354262583 1103 9 0.829750038799304 1103 10 0.7405610889791063 1103 11 0.8012702103320564 1103 12 1.0841069353461066 1103 13 0.8796917410757459 1103 14 1.1164090628940002 1103 15 0.749074848982751 1103 16 0.8300380187496188 1103 17 1.134651051313 1103 18 1.1356339285702006 1103 19 1.0068098580406328 1103 20 1.0647954371088488 1103 21 1.2182033119698445 1103 22 1.358579103857636 1103 23 1.191487556647843 1103 24 1.3661048508925333 1103 25 1.0254366700491744 1104 1 1.546286826702886 1104 2 4.14727188278561 1104 3 2.6587881207771886 1104 4 1.8381349036267736 1104 5 1.3869786669071473 1104 6 1.1888365347969057 1104 7 1.0662147337089536 1104 8 1.0904175246791894 1104 9 0.9785317549113088 1104 10 1.0472320456221524 1104 11 1.1592325192676185 1104 12 1.117070350392865 1104 13 1.027978309762652 1104 14 1.2325546427435157 1104 15 1.3182962638160944 1104 16 1.1392936970039926 1104 17 1.2625539425575454 1104 18 1.2027608893149662 1104 19 1.3923676894380534 1104 20 1.307009390246165 1104 21 1.3808699769014368 1104 22 1.4539265398918815 1104 23 1.5376697813107398 1104 24 1.5296655139346242 1104 25 1.6579468397656711 1105 1 1.4125311586917597 1105 2 3.771899234540502 1105 3 2.3786305438049737 1105 4 1.54028686895483 1105 5 1.1015075643693777 1105 6 0.9233475200913972 1105 7 0.8462664660535708 1105 8 0.7290119831805768 1105 9 0.726760579722793 1105 10 0.6755163605756778 1105 11 0.7491090129920934 1105 12 0.8454317001937 1105 13 0.877378314773047 1105 14 0.6445219749578612 1105 15 0.776668010189816 1105 16 0.6951420824036916 1105 17 0.7929620393584074 1105 18 0.7190440518772832 1105 19 0.767749105877165 1105 20 0.7376899273606767 1105 21 0.866799530725622 1105 22 0.7780102636186115 1105 23 0.82947151991835 1105 24 0.8364508434934592 1105 25 0.8753466944641701 1106 1 1.2201294865814774 1106 2 3.792837528889571 1106 3 2.1617252135139715 1106 4 1.3474548640045967 1106 5 0.8283137141913031 1106 6 0.690928058077759 1106 7 0.5293931294622709 1106 8 0.47353104052664463 1106 9 0.4349748742265547 1106 10 0.4780438628521537 1106 11 0.5865043742572169 1106 12 0.584080089433975 1106 13 0.5683525756658483 1106 14 0.6295341571742838 1106 15 0.8020745235445546 1106 16 0.5832963271941694 1106 17 0.6156118208519814 1106 18 0.6557042612074895 1106 19 0.518979456999233 1106 20 0.5789790273532986 1106 21 0.632316299291638 1106 22 0.5872993354849001 1106 23 0.6785973184424208 1106 24 0.7154826649193922 1106 25 0.4579867723740989 1107 1 1.4263578154436587 1107 2 3.8061496842007934 1107 3 2.446476178445657 1107 4 1.670695344812998 1107 5 1.2453238614565976 1107 6 1.0494602307008378 1107 7 0.8544582278956838 1107 8 0.8211545270820366 1107 9 0.793987812294084 1107 10 0.8323712172246118 1107 11 0.8967323279576789 1107 12 0.9348616644115495 1107 13 1.0551800320622462 1107 14 1.026927069624854 1107 15 1.105685240282419 1107 16 1.0606831683253546 1107 17 1.1010832537907547 1107 18 1.0531762869590509 1107 19 1.2019760397342054 1107 20 1.1596949991474332 1107 21 1.245189143096205 1107 22 1.2640463254206864 1107 23 1.3245058720579692 1107 24 1.3724992987594078 1107 25 1.3659683379010374 1108 1 1.2513235770575335 1108 2 3.875005071402523 1108 3 2.3778073813905216 1108 4 1.431836406963825 1108 5 1.169946876898294 1108 6 0.866248633851491 1108 7 0.6648753927399227 1108 8 0.7153651601842199 1108 9 0.6629761676196964 1108 10 0.4556620152507449 1108 11 0.5753588795543862 1108 12 0.6010301535023643 1108 13 0.7327377663144006 1108 14 0.6913437852035003 1108 15 0.708117796096424 1108 16 0.6611320716100693 1108 17 0.7191223543900165 1108 18 0.842948973060075 1108 19 0.6518575640488962 1108 20 0.7254290773921142 1108 21 0.7399809229850476 1108 22 0.7753160079638164 1108 23 0.828585493515476 1108 24 0.843764416184392 1108 25 0.9380754660069428 1109 1 0.9453075677737317 1109 2 2.9607879306553357 1109 3 1.7632269327613308 1109 4 1.009123400409777 1109 5 0.7633585423121758 1109 6 0.5441376238423956 1109 7 0.42502295771994625 1109 8 0.3787580997471949 1109 9 0.483775049343528 1109 10 0.4843508100708007 1109 11 0.4496121532814854 1109 12 0.32303039087230445 1109 13 0.506329701011289 1109 14 0.5533263517000506 1109 15 0.41938693404792105 1109 16 0.4192586952864019 1109 17 0.5899964032618499 1109 18 0.30435987361526173 1109 19 0.5115917097149136 1109 20 0.5205953022869423 1109 21 0.7044083998744455 1109 22 0.5007053606032414 1109 23 0.576327182270731 1109 24 0.38211284888342334 1109 25 0.34735069645359573 1110 1 0.9078765981740773 1110 2 3.3938974208572716 1110 3 2.045712193137156 1110 4 1.1541525892221784 1110 5 0.581486516687427 1110 6 0.44787366735799594 1110 7 0.2347210288103696 1110 8 0.2742706569527016 1110 9 0.20079915967599504 1110 10 0.19012882572945244 1110 11 0.11550605288193339 1110 12 0.09357043576540747 1110 13 0.14991500827177617 1110 14 0.17171322897426933 1110 15 0.06369625662682665 1110 16 0.24561845414158512 1110 17 0.2651645449282043 1110 18 0.2781729241469648 1110 19 0.37764991307815876 1110 20 0.19350322994350222 1110 21 0.1157185979102735 1110 22 0.0973209520345506 1110 23 0.2429907371237121 1110 24 0.31764955376316095 1110 25 0.16787476736976004 1111 1 0.4244463789570183 1111 2 3.0427778789745226 1111 3 1.5766631535822206 1111 4 0.7185602850209065 1111 5 0.2504407100614472 1111 6 -0.10393580400202751 1111 7 -0.163644404346023 1111 8 -0.23628149059939574 1111 9 -0.40724797785606626 1111 10 -0.46626793726218096 1111 11 -0.48333871043605114 1111 12 -0.46606843485114524 1111 13 -0.477864979184055 1111 14 -0.47098259524413777 1111 15 -0.5974915133150915 1111 16 -0.5364214526439994 1111 17 -0.6168422166866279 1111 18 -0.6539327630544136 1111 19 -0.48042068103627855 1111 20 -0.48601968356800285 1111 21 -0.4051082976110507 1111 22 -0.502062802752377 1111 23 -0.46997692848741224 1111 24 -0.4404465366364718 1111 25 -0.43582933294283066 1112 1 1.0090840106191479 1112 2 3.3748855751763784 1112 3 2.078761633283513 1112 4 1.075489313122148 1112 5 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-2.7161418441859837 2315 17 -2.5303112654018634 2315 18 -2.628870813264598 2315 19 -2.67114168107598 2315 20 -2.6293774533970335 2315 21 -2.728524083995083 2315 22 -2.6997715459094636 2315 23 -2.7982053201109593 2315 24 -2.623887886363825 2315 25 -2.777765986935144 2316 1 -0.7770132652347463 2316 2 1.5612379596754913 2316 3 0.08193107441435643 2316 4 -0.7974074345911362 2316 5 -1.0307287087425152 2316 6 -1.215246715667469 2316 7 -1.6381565254613548 2316 8 -1.6240209052512036 2316 9 -1.8376652167756546 2316 10 -2.0542234772027754 2316 11 -1.8847962253146306 2316 12 -1.9316959966810785 2316 13 -1.9803786334048823 2316 14 -2.302351637159667 2316 15 -2.172486988524561 2316 16 -2.3729114927815544 2316 17 -2.3251114044601167 2316 18 -2.293961721713792 2316 19 -2.3590104369105163 2316 20 -2.163813076309175 2316 21 -2.380132518548585 2316 22 -2.310886676611865 2316 23 -2.332273179831539 2316 24 -2.493045895713202 2316 25 -2.45932523933822 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74995.0 3 1.0 4 673.0 5 3195.0 6 3855.0 7 3517.0 8 3197.0 9 3203.0 10 3063.0 11 2996.0 12 2951.0 13 2940.0 14 2999.0 15 3140.0 16 3198.0 17 3518.0 18 4053.0 19 4846.0 20 6174.0 21 7989.0 22 10102.0 23 11876.0 24 13783.0 25 15466.0 26 17556.0 27 19917.0 28 23424.0 29 27815.0 30 34810.0 31 44159.0 32 56821.0 33 75017.0 34 101426.0 35 143701.0 36 223733.0 37 285559.0 38 671168.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76686372751764 18.185958527490094 13.053020707040144 24.994157037952125 2 17.287160229814987 20.740764570498207 38.32890675393689 23.64316844574991 3 18.922328253434305 24.094027866755425 29.214132038421646 27.76951184138862 4 12.970541037417913 16.52316630113374 37.097435565692635 33.408857095755714 5 14.186764021543835 36.95908257148759 34.74183498223113 14.112318424737433 6 32.620735420244614 35.967291933164866 17.752222934043395 13.659749712547134 7 28.739808726463817 31.362829162223584 21.521194301442588 18.376167809870015 8 26.21601430690993 35.153189944262316 20.20616265554278 18.424633093284974 9 26.715796239219213 15.357443203278736 19.79532938655159 38.131431170950464 10 16.090682770983015 28.16307707075618 33.04372413706754 22.702516021193258 11 34.70437742195994 22.54752101901258 23.648136825476985 19.099964733550497 12 23.88295086277595 24.287523815287276 30.423364335811726 21.40616098612505 13 29.126174591879533 21.470329230043674 25.447560459006404 23.955935719070386 14 22.17670160618853 21.227481119928882 26.97841651554958 29.617400758333 15 23.979307567261884 28.857502676285296 23.477282354880646 23.685907401572173 16 23.364231473115073 27.378920262348995 26.02528333149002 23.231564933045913 17 22.656294017850247 27.385024070916863 26.891450285783446 23.067231625449438 18 22.65452026151429 26.771200040066024 28.13292321304483 22.441356485374857 19 23.89041107324779 26.44701998501698 27.3415670406858 22.321001901049435 20 24.15063156159421 26.20479790655017 27.46171294779522 22.1828575840604 21 23.983506135638244 25.875532257879396 27.008598553656704 23.13236305282565 22 24.164098615260773 26.3206879299758 26.950114851501677 22.565098603261752 23 23.63328752844546 26.23180194759462 27.037300203565685 23.097610320394235 24 23.759622628122592 26.046829254041555 27.320125456742257 22.873422661093592 25 23.650953967892924 26.26917482768479 27.215317325008503 22.864553879413783 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 276.0 1 276.0 2 454.5 3 633.0 4 633.0 5 633.0 6 1605.0 7 2577.0 8 2577.0 9 2577.0 10 3402.0 11 4227.0 12 4227.0 13 4227.0 14 6044.5 15 7862.0 16 7862.0 17 7862.0 18 13663.0 19 19464.0 20 19464.0 21 19464.0 22 29744.0 23 40024.0 24 40024.0 25 40024.0 26 56955.0 27 73886.0 28 73886.0 29 73886.0 30 95363.0 31 116840.0 32 116840.0 33 116840.0 34 134553.0 35 152266.0 36 152266.0 37 152266.0 38 170316.5 39 188367.0 40 188367.0 41 188367.0 42 206263.0 43 224159.0 44 224159.0 45 224159.0 46 238239.0 47 252319.0 48 252319.0 49 252319.0 50 252678.0 51 253037.0 52 253037.0 53 253037.0 54 234572.0 55 216107.0 56 216107.0 57 216107.0 58 188103.0 59 160099.0 60 160099.0 61 160099.0 62 132980.5 63 105862.0 64 105862.0 65 105862.0 66 81959.0 67 58056.0 68 58056.0 69 58056.0 70 42151.0 71 26246.0 72 26246.0 73 26246.0 74 18061.5 75 9877.0 76 9877.0 77 9877.0 78 6589.5 79 3302.0 80 3302.0 81 3302.0 82 2111.5 83 921.0 84 921.0 85 921.0 86 616.5 87 312.0 88 312.0 89 312.0 90 197.5 91 83.0 92 83.0 93 83.0 94 55.0 95 27.0 96 27.0 97 27.0 98 17.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.321652973963343 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0433860799776298E-4 22 6.782009519854593E-4 23 5.216930399888148E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1916836.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.10462482264788 #Duplication Level Percentage of deduplicated Percentage of total 1 76.93184735384159 50.85550906238914 2 14.428675114871298 19.076043103128857 3 4.529358703805504 8.982346734067725 4 1.8314054719863697 4.842574864952132 5 0.8423428453145729 2.784137888078078 6 0.4603578239975359 1.8259068743716607 7 0.25234762501282015 1.1676941553451095 8 0.17914509275630378 0.9473855316379131 9 0.11165680504706257 0.6642928085878447 >10 0.398006572978378 4.342040819059309 >50 0.01968272755360655 0.8957970414918736 >100 0.013337786902659184 1.7892891760557157 >500 8.78123271987515E-4 0.4261779311452872 >1k 8.78123271987515E-4 1.051091138255551 >5k 7.982938836250138E-5 0.3497128714338556 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6627 0.3457259776005876 No Hit GGTATCAACGCAGAGTACTTTTTTT 4047 0.2111291732834734 No Hit TATCAACGCAGAGTACTTTTTTTTT 4002 0.2087815546035237 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.5650791199664447E-4 13 0.0 0.0 0.0 0.0 2.0867721599552596E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATA 55 1.400295E-4 14.376718 1 ACCGTCC 60 2.6817252E-5 14.185165 8 GGTATCA 1260 0.0 13.884658 1 CCCTTAT 105 1.6258127E-7 12.237326 1 TTACCCG 55 0.003175494 12.035897 4 ACCGTGC 95 1.0990625E-6 11.945402 8 GGACCGG 65 8.3377893E-4 11.63911 6 CGTGCGC 75 2.1640143E-4 11.348132 10 TAGGACC 445 0.0 10.838103 4 TGTCGAA 70 0.001549502 10.807746 14 TATGACT 185 5.456968E-12 10.73472 4 GTAATAC 150 2.2992026E-9 10.71768 3 ACCGCAT 80 3.9338192E-4 10.638874 8 GTAGGAC 810 0.0 10.50753 3 TCGAAAC 90 9.966999E-5 10.50753 16 GTATCAA 2640 0.0 10.483024 1 ATAAGAC 165 1.0059011E-9 10.3164835 3 TAGACTG 225 0.0 10.087229 5 GTCGAAA 85 6.876695E-4 10.013058 15 CTAGCAC 170 1.6862032E-9 10.013058 3 >>END_MODULE