FastQCFastQC Report
Thu 26 May 2016
SRR1511736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916836
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86570.451629664718317No Hit
TATCAACGCAGAGTACTTTTTTTTT56640.2954869378496648No Hit
GGTATCAACGCAGAGTACTTTTTTT54510.284374876097903No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25750.13433595779711985No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21030.10971204630964776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.014.7261941
CGTCCAA658.046485E-411.68856710
GTATCAA34050.011.0669761
GTAGGAC9950.010.9806843
CGCCCTA1752.0008883E-1110.8536716
TAGGACC3800.010.7507684
TGTAGGA10100.010.7235382
GGCGAGG3900.010.47100919
TAGGACA5000.010.4507474
ATAGGAC2250.010.1340563
TAGGACG8400.010.06619554
CGAATGG951.6495935E-49.99680119
CTTACAC2002.5465852E-119.9757133
GACGTGG5150.09.5891657
TTAGGAC5450.09.587843
AGGACGT8650.09.5555955
GTATTAG1606.218215E-89.5400121
GTACTAG1106.529604E-59.5400121
TCTTACG800.0045136019.5006782
TACTAGA1304.234813E-69.5006782
Produced by FastQC (version 0.11.5)