##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511736_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1916836 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.426960887629406 33.0 31.0 34.0 28.0 34.0 2 31.602514247436922 33.0 31.0 34.0 28.0 34.0 3 31.70384059982179 34.0 31.0 34.0 28.0 34.0 4 35.24448466118124 37.0 35.0 37.0 32.0 37.0 5 34.93889513761219 37.0 35.0 37.0 32.0 37.0 6 34.90521359156443 37.0 35.0 37.0 32.0 37.0 7 34.761854952640704 37.0 35.0 37.0 32.0 37.0 8 34.68801347637461 37.0 35.0 37.0 32.0 37.0 9 36.26219092295846 39.0 37.0 39.0 32.0 39.0 10 36.106698225617635 39.0 37.0 39.0 32.0 39.0 11 36.10876099989775 39.0 37.0 39.0 32.0 39.0 12 35.961578872683944 39.0 37.0 39.0 31.0 39.0 13 35.94086035529383 39.0 37.0 39.0 31.0 39.0 14 37.073078761041636 40.0 37.0 41.0 31.0 41.0 15 36.99381637239701 40.0 37.0 41.0 31.0 41.0 16 36.853666145669216 40.0 37.0 41.0 31.0 41.0 17 36.843026216118645 40.0 37.0 41.0 31.0 41.0 18 36.790260616975054 40.0 37.0 41.0 31.0 41.0 19 36.80120260679578 40.0 37.0 41.0 30.0 41.0 20 36.89459922497282 40.0 37.0 41.0 31.0 41.0 21 36.84518759038332 40.0 37.0 41.0 31.0 41.0 22 36.76023874760282 40.0 37.0 41.0 31.0 41.0 23 36.664485641964156 40.0 37.0 41.0 30.0 41.0 24 36.532887007547856 40.0 37.0 41.0 30.0 41.0 25 36.04968239327725 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09263092807315587 1101 2 0.17360668903237553 1101 3 0.1415038525001613 1101 4 0.13036205422637437 1101 5 0.25145637425539036 1101 6 0.10243083325985225 1101 7 0.13878301930567005 1101 8 0.18671840844900345 1101 9 0.099700314272404 1101 10 -0.03262141360112025 1101 11 0.06015430328475446 1101 12 0.16216645924473738 1101 13 0.14060087712858405 1101 14 -0.026344504739881813 1101 15 0.07547610814011563 1101 16 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0.46328903315100334 1103 4 0.45451745575795144 1103 5 0.5345076014391879 1103 6 0.45663949243387236 1103 7 0.3799623435563362 1103 8 0.36239206734959595 1103 9 0.4546435372859108 1103 10 0.5084028478217562 1103 11 0.525363250004176 1103 12 0.6990578288252607 1103 13 0.7205683121706699 1103 14 0.7492621190360609 1103 15 0.806565764091161 1103 16 0.9623634239882364 1103 17 0.9416245840248365 1103 18 0.7412672297997247 1103 19 0.9129323611554057 1103 20 0.8860641745545124 1103 21 1.055813198318063 1103 22 0.9630981739980555 1103 23 1.023653464298782 1103 24 0.9198618592897887 1103 25 1.105679956870624 1104 1 0.19106108059404292 1104 2 0.32118461916445185 1104 3 0.3028352412716764 1104 4 0.3215719987518426 1104 5 0.17039336227452395 1104 6 0.4877498458949603 1104 7 0.4869398881976821 1104 8 0.5762443533561168 1104 9 0.5689845292464355 1104 10 0.6352646932316546 1104 11 0.5439716189716464 1104 12 0.6152959316233293 1104 13 0.44211620418298025 1104 14 0.7458647310200632 1104 15 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-0.5904796175942337 2313 10 -0.5959033540644185 2313 11 -0.6042749945849621 2313 12 -0.7868725141916428 2313 13 -0.6581311290291154 2313 14 -0.6878887560203353 2313 15 -0.6547997034677309 2313 16 -0.6353171970767164 2313 17 -0.6019439555080623 2313 18 -0.7035332841822992 2313 19 -0.529811022903445 2313 20 -0.650123885578985 2313 21 -0.6971584480418755 2313 22 -0.7693547531305711 2313 23 -0.6591814690523066 2313 24 -0.6665328402455515 2313 25 -0.5312721657839958 2314 1 -0.4796586214787446 2314 2 -0.48926689067984697 2314 3 -0.5508282241529834 2314 4 -0.5285405900200715 2314 5 -0.5930653263068564 2314 6 -0.4990628333328928 2314 7 -0.7174505927584605 2314 8 -0.5893698809440053 2314 9 -0.6877789765947071 2314 10 -1.6309894687037172 2314 11 -1.0612984016215137 2314 12 -0.8851168938736933 2314 13 -0.8890598192200869 2314 14 -0.8477124163461269 2314 15 -0.8088684396506949 2314 16 -0.7230963614443979 2314 17 -0.7971903385450858 2314 18 -0.8962564027505451 2314 19 -0.8814561069073363 2314 20 -0.8299307340164788 2314 21 -0.8274628024690642 2314 22 -0.79201006868675 2314 23 -0.6845807513994586 2314 24 -0.9379933637840523 2314 25 -0.9850620616169437 2315 1 -0.843372887022884 2315 2 -0.8811145564118803 2315 3 -0.8071143930428057 2315 4 -0.8760977135604193 2315 5 -1.08062132755947 2315 6 -1.1001943527837668 2315 7 -1.1622003678483779 2315 8 -1.03250193257648 2315 9 -1.2604324449163258 2315 10 -1.3752434460371248 2315 11 -1.367800248870914 2315 12 -1.314856432613439 2315 13 -1.1756126400540907 2315 14 -1.452039740613067 2315 15 -1.4953714815934802 2315 16 -1.4128670708207522 2315 17 -1.6889668657162176 2315 18 -1.5036309192164978 2315 19 -1.505828576103255 2315 20 -1.7346644350498863 2315 21 -1.780466641779185 2315 22 -1.8294456050226273 2315 23 -1.7697732508971384 2315 24 -1.783700703726815 2315 25 -1.935057988798576 2316 1 -1.0177644630575706 2316 2 -1.0122309576972839 2316 3 -1.0157661809472067 2316 4 -1.0086454618932237 2316 5 -1.1438635377386106 2316 6 -1.1855713541725876 2316 7 -1.2794273557372478 2316 8 -1.9572744188074154 2316 9 -1.5207076673124647 2316 10 -1.6237292463897148 2316 11 -1.495566722773674 2316 12 -1.6945948689845807 2316 13 -1.7549441369243937 2316 14 -1.7235936812733144 2316 15 -1.7991013485881453 2316 16 -1.704837162708607 2316 17 -1.8653516688376186 2316 18 -2.0358634923764924 2316 19 -1.9051241084157695 2316 20 -1.9006258693263334 2316 21 -1.8652148810511449 2316 22 -1.8600331261328336 2316 23 -1.9454082335766216 2316 24 -1.902105275002107 2316 25 -1.840051556472119 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20522.0 3 0.0 4 341.0 5 3328.0 6 4477.0 7 3357.0 8 2881.0 9 2642.0 10 2463.0 11 2401.0 12 2198.0 13 2264.0 14 2266.0 15 2172.0 16 2287.0 17 2415.0 18 2556.0 19 2866.0 20 3623.0 21 4528.0 22 5889.0 23 7509.0 24 9381.0 25 11040.0 26 13323.0 27 15004.0 28 17633.0 29 21911.0 30 27788.0 31 36297.0 32 47481.0 33 62480.0 34 83988.0 35 113939.0 36 169724.0 37 312329.0 38 891533.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.86370707166498 18.711244843779554 13.575379450146363 24.849668634409102 2 15.553130262578541 20.1585842502958 39.53410724756839 24.75417823955727 3 18.741144260646188 24.369273114653524 29.728834391674614 27.16074823302567 4 13.17212322806959 16.940155547996802 36.903730939944786 32.983990283988824 5 13.997990788941511 37.1320469163829 34.76717940689134 14.102782887784256 6 32.51133638975895 36.277751461262206 17.603540417646578 13.607371731332258 7 28.53467902314022 31.519024058396234 21.548635355345997 18.397661563117556 8 26.035821530897792 35.48305645344724 20.07088765027368 18.410234365381285 9 26.619230857517284 15.376380660630332 19.805867585959362 38.198520895893026 10 15.967615382849655 28.37629301619961 33.08780719894659 22.568284402004135 11 34.737191914175234 22.51126335273336 23.645006667237052 19.106538065854355 12 23.77741236078621 24.384297874205203 30.530311409009432 21.307978355999158 13 29.22712219511737 21.409030297844993 25.524092827972762 23.839754679064875 14 22.027914751183722 21.24902704248042 27.13570696710621 29.587351239229648 15 23.88394207955193 28.90424637266829 23.61057492659779 23.60123662118199 16 23.345815708803467 27.495153471658508 26.063366923409205 23.09566389612883 17 22.58064852705187 27.46176511709922 26.95102763095017 23.00655872489874 18 22.584769902067784 26.68590322802785 28.297778213681298 22.431548656223068 19 23.791863257993903 26.400380627241976 27.566677587440967 22.24107852732315 20 24.075246917315827 26.182104259310655 27.615247209463927 22.12740161390959 21 23.908722498951395 25.814728020550533 27.26070461948753 23.01584486101054 22 23.98875021128568 26.369652907186637 27.147288552593963 22.49430832893372 23 23.51432256071985 26.147620349367394 27.4020834333245 22.935973656588253 24 23.68376846010822 25.960593394531408 27.52629854614584 22.82933959921454 25 23.54248193476325 26.189441481466798 27.55249235315262 22.715584230617335 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 273.0 1 273.0 2 513.0 3 753.0 4 753.0 5 753.0 6 1775.5 7 2798.0 8 2798.0 9 2798.0 10 3556.0 11 4314.0 12 4314.0 13 4314.0 14 6201.0 15 8088.0 16 8088.0 17 8088.0 18 14220.0 19 20352.0 20 20352.0 21 20352.0 22 30943.0 23 41534.0 24 41534.0 25 41534.0 26 59269.5 27 77005.0 28 77005.0 29 77005.0 30 97808.0 31 118611.0 32 118611.0 33 118611.0 34 136815.0 35 155019.0 36 155019.0 37 155019.0 38 171894.0 39 188769.0 40 188769.0 41 188769.0 42 206023.0 43 223277.0 44 223277.0 45 223277.0 46 237632.0 47 251987.0 48 251987.0 49 251987.0 50 251730.5 51 251474.0 52 251474.0 53 251474.0 54 232539.5 55 213605.0 56 213605.0 57 213605.0 58 186030.0 59 158455.0 60 158455.0 61 158455.0 62 131694.0 63 104933.0 64 104933.0 65 104933.0 66 80848.5 67 56764.0 68 56764.0 69 56764.0 70 40994.5 71 25225.0 72 25225.0 73 25225.0 74 17266.0 75 9307.0 76 9307.0 77 9307.0 78 6213.0 79 3119.0 80 3119.0 81 3119.0 82 2000.0 83 881.0 84 881.0 85 881.0 86 557.5 87 234.0 88 234.0 89 234.0 90 142.5 91 51.0 92 51.0 93 51.0 94 29.5 95 8.0 96 8.0 97 8.0 98 4.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42877950956680694 2 0.0 3 0.0 4 0.0 5 0.03448390994326067 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0017215870319630892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1916836.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.542665977530646 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4753549822791 46.57887250214704 2 15.412335627751247 19.278571182000825 3 5.18208511840702 9.72304255882988 4 2.138977043314686 5.351093070145461 5 1.0180654612295823 3.1836264042471223 6 0.5683033650208769 2.1325924519444426 7 0.324771815714354 1.4218466632397286 8 0.19496366778079716 0.975483804141492 9 0.14025922340969138 0.7894967183982157 >10 0.5036500729173116 5.137045728811674 >50 0.022446134071943288 0.9691496585937107 >100 0.016366994512575957 2.024870371937214 >500 0.0012519794435003509 0.5524998444191892 >1k 9.181182585669054E-4 0.8496825366223558 >5k 2.5039588870006513E-4 1.0321265045215469 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8657 0.451629664718317 No Hit TATCAACGCAGAGTACTTTTTTTTT 5664 0.2954869378496648 No Hit GGTATCAACGCAGAGTACTTTTTTT 5451 0.284374876097903 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2575 0.13433595779711985 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2103 0.10971204630964776 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.216930399888149E-5 7 0.0 0.0 0.0 0.0 5.216930399888149E-5 8 0.0 0.0 0.0 0.0 5.216930399888149E-5 9 0.0 0.0 0.0 5.216930399888149E-5 5.216930399888149E-5 10 0.0 0.0 0.0 5.216930399888149E-5 5.216930399888149E-5 11 0.0 0.0 0.0 5.216930399888149E-5 5.216930399888149E-5 12 0.0 0.0 0.0 5.216930399888149E-5 2.0867721599552596E-4 13 0.0 0.0 0.0 5.216930399888149E-5 2.0867721599552596E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1490 0.0 14.726194 1 CGTCCAA 65 8.046485E-4 11.688567 10 GTATCAA 3405 0.0 11.066976 1 GTAGGAC 995 0.0 10.980684 3 CGCCCTA 175 2.0008883E-11 10.85367 16 TAGGACC 380 0.0 10.750768 4 TGTAGGA 1010 0.0 10.723538 2 GGCGAGG 390 0.0 10.471009 19 TAGGACA 500 0.0 10.450747 4 ATAGGAC 225 0.0 10.134056 3 TAGGACG 840 0.0 10.0661955 4 CGAATGG 95 1.6495935E-4 9.996801 19 CTTACAC 200 2.5465852E-11 9.975713 3 GACGTGG 515 0.0 9.589165 7 TTAGGAC 545 0.0 9.58784 3 AGGACGT 865 0.0 9.555595 5 GTATTAG 160 6.218215E-8 9.540012 1 GTACTAG 110 6.529604E-5 9.540012 1 TCTTACG 80 0.004513601 9.500678 2 TACTAGA 130 4.234813E-6 9.500678 2 >>END_MODULE