FastQCFastQC Report
Thu 26 May 2016
SRR1511735_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511735_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315844
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44980.3418338344059022No Hit
GGTATCAACGCAGAGTACTTTTTTT26920.20458352205884586No Hit
TATCAACGCAGAGTACTTTTTTTTT26070.19812378975015274No Hit
GTCCTACAGTGGACATTTCTAAATT13210.10039183976216026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG456.9990725E-414.7105687
AATCCCG1500.013.24001519
ATCGCGC500.001551720413.2395128
CGCGCCT604.2516348E-412.60905912
GGTCTAT600.004375422411.5302211
CCAACGA752.1622327E-411.34858419
ACTGTTC4250.011.3481538
TAGGACC2750.011.3481534
CTATACT2100.011.2580884
GTGTAAG1851.8189894E-1211.2185941
GTCCTAA4150.011.1928481
TCCAACG1105.2640644E-711.17663718
GTTAGAT600.006077548511.03292753
TCGCGCG600.006077548511.03292759
TGGCGAG6800.010.98698718
GGCGAGG3550.010.92234619
GTATCAA18000.010.8164451
TGTAGGA9050.010.5928792
GTCTAAA1405.423317E-810.5889791
CTGTAGG8600.010.5725621
Produced by FastQC (version 0.11.5)