##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511735_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1315844 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.85826663343071 31.0 28.0 34.0 16.0 34.0 2 26.240668346703714 31.0 25.0 34.0 10.0 34.0 3 27.876836463896936 31.0 26.0 34.0 16.0 34.0 4 32.31039393727524 35.0 32.0 37.0 22.0 37.0 5 32.7486799347035 35.0 33.0 37.0 26.0 37.0 6 32.992582707372605 37.0 35.0 37.0 26.0 37.0 7 33.01520241001213 37.0 35.0 37.0 26.0 37.0 8 33.030413179677836 37.0 35.0 37.0 26.0 37.0 9 34.56953179860226 39.0 35.0 39.0 27.0 39.0 10 34.432786105343794 39.0 35.0 39.0 27.0 39.0 11 34.443241752061795 39.0 35.0 39.0 26.0 39.0 12 34.336556613093954 39.0 35.0 39.0 26.0 39.0 13 34.321960657950335 39.0 35.0 39.0 26.0 39.0 14 35.42129538151939 40.0 36.0 41.0 26.0 41.0 15 35.3524323552032 40.0 36.0 41.0 25.0 41.0 16 35.287275695295186 40.0 36.0 41.0 25.0 41.0 17 35.25624314128422 40.0 36.0 41.0 25.0 41.0 18 35.18216521107365 40.0 36.0 41.0 25.0 41.0 19 35.210892780603174 40.0 36.0 41.0 25.0 41.0 20 35.123709193491024 40.0 36.0 41.0 24.0 41.0 21 35.029705648997904 40.0 35.0 41.0 23.0 41.0 22 34.91674545006855 40.0 35.0 41.0 22.0 41.0 23 34.80206392247105 40.0 35.0 41.0 20.0 41.0 24 34.68068783229623 39.0 35.0 41.0 19.0 41.0 25 34.56328865731804 39.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2848185212708358 1101 2 3.8305298594725734 1101 3 2.4836406936520206 1101 4 1.5367394133864636 1101 5 1.1544648440808558 1101 6 0.917140848416885 1101 7 0.8533672233097604 1101 8 0.7032062395533245 1101 9 0.7489595288756306 1101 10 1.0221907150491916 1101 11 0.9026022145507042 1101 12 0.8771600549246656 1101 13 0.7626964085898962 1101 14 1.0579449854424254 1101 15 0.9922948444927968 1101 16 0.9302795642973365 1101 17 1.0269828149623308 1101 18 0.8102813742384285 1101 19 0.9904739038506278 1101 20 0.9447088522674392 1101 21 1.1388294534065935 1101 22 1.1127938537652682 1101 23 1.0096190723337841 1101 24 1.134107847060882 1101 25 1.0681216717539002 1102 1 1.4691460320823673 1102 2 4.065380430852823 1102 3 2.776557028120866 1102 4 1.7422671851242768 1102 5 1.375396930766371 1102 6 1.1593625874422742 1102 7 0.9020541359236134 1102 8 0.9805902119144676 1102 9 1.149824451201745 1102 10 1.112062909487598 1102 11 1.0602427644286934 1102 12 1.1838813228331517 1102 13 0.9470819030210507 1102 14 1.237109538122965 1102 15 1.1045197242975888 1102 16 1.360426214535309 1102 17 1.316197861711629 1102 18 1.2239026928825751 1102 19 1.130209595184489 1102 20 1.1468856539876242 1102 21 1.3209849944662437 1102 22 1.1633217463022945 1102 23 1.346692349133086 1102 24 1.3334893551181892 1102 25 1.1479651159809094 1103 1 1.5079353049540352 1103 2 4.015355202924976 1103 3 2.745776669625151 1103 4 1.964309534507457 1103 5 1.3137249365101056 1103 6 1.1517911603951916 1103 7 0.9820838301855233 1103 8 0.7234467450331721 1103 9 0.8854311942991231 1103 10 0.7157778933523957 1103 11 0.8101303439042908 1103 12 0.9817842799121337 1103 13 0.8382626462617253 1103 14 1.199795070096883 1103 15 0.9601450949042345 1103 16 0.8697727426084327 1103 17 1.0201599420782017 1103 18 1.1237775440682896 1103 19 1.163900692546001 1103 20 1.1887007538551586 1103 21 1.2558649293461812 1103 22 1.450327153157886 1103 23 1.1514967831510035 1103 24 1.1660231689254132 1103 25 0.9892623954804378 1104 1 1.3686693325677481 1104 2 3.897605857022789 1104 3 2.399205345449431 1104 4 1.591301359568753 1104 5 1.010164854159079 1104 6 0.9819267637132967 1104 7 0.8156568404148317 1104 8 0.6827877607447235 1104 9 0.9136935025637172 1104 10 0.725184722383041 1104 11 0.8441903873689611 1104 12 0.782044891851271 1104 13 0.7271892801855913 1104 14 0.9817908351528715 1104 15 1.0128823273404421 1104 16 0.8588691558192281 1104 17 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5 1.2238484689685976 1106 6 1.096363960270054 1106 7 0.8428662050828706 1106 8 0.7632095282364588 1106 9 0.7148432359804531 1106 10 0.6809974290223053 1106 11 0.9493344019044514 1106 12 0.844893013854076 1106 13 0.7917203021377546 1106 14 0.9409842346107666 1106 15 1.247564959217243 1106 16 0.9912821067023785 1106 17 0.8825919170253513 1106 18 1.0154673745672937 1106 19 0.7056638253016985 1106 20 0.944774625930151 1106 21 0.7622929426994034 1106 22 0.7964933693169414 1106 23 0.8110989797447061 1106 24 0.8275949895747061 1106 25 0.8518300414524802 1107 1 1.652659680770146 1107 2 3.9821984571235873 1107 3 2.629461816349039 1107 4 1.755678962217651 1107 5 1.3258431125558374 1107 6 1.2281588179300513 1107 7 1.05357168047599 1107 8 1.0022543778572413 1107 9 1.131437549730265 1107 10 1.2352827889263835 1107 11 1.2086541466709235 1107 12 1.1735888697546244 1107 13 0.9637709445857183 1107 14 0.9627406418635189 1107 15 1.0433046357193163 1107 16 1.35514837094901 1107 17 1.2651436091648378 1107 18 1.1060922740771346 1107 19 1.3032070418137778 1107 20 1.3890215354269841 1107 21 1.4276342876827002 1107 22 1.332212741063934 1107 23 1.3829396838878836 1107 24 1.3885184424855836 1107 25 1.351708304798727 1108 1 1.4211606418002738 1108 2 3.916951167978251 1108 3 2.5403471144622642 1108 4 1.6054461046814765 1108 5 1.15818116249838 1108 6 0.9930993788304079 1108 7 0.6957970307317254 1108 8 0.7262700490466258 1108 9 0.6818131076383054 1108 10 0.5197431837565958 1108 11 0.7904392973012477 1108 12 0.8315442216002324 1108 13 0.9161530972156555 1108 14 0.8546244289555958 1108 15 1.0565961930017522 1108 16 1.0349585949653246 1108 17 1.004805910802915 1108 18 1.0222396484032146 1108 19 0.8660566027904011 1108 20 0.9965800018633146 1108 21 0.802660300771322 1108 22 0.9291126271880046 1108 23 0.9656781091944495 1108 24 1.0420867919567272 1108 25 1.0695402932069058 1109 1 1.306091890789233 1109 2 3.26164211931577 1109 3 2.227219568468943 1109 4 1.549042334037793 1109 5 1.131271943245153 1109 6 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-0.6508892412862401 2316 2 1.6671548346562126 2316 3 0.33251899422776177 2316 4 -0.6654285437308687 2316 5 -1.0075522295740953 2316 6 -1.19426673546241 2316 7 -1.588626026640604 2316 8 -1.4371630278677614 2316 9 -1.82045083443586 2316 10 -1.7103326123282159 2316 11 -1.8159921868787237 2316 12 -1.9587105029470848 2316 13 -1.9768549116403946 2316 14 -2.20750667229035 2316 15 -2.4074272122870326 2316 16 -2.334910032684995 2316 17 -2.4314487911524196 2316 18 -2.497465300369477 2316 19 -2.4248367969633478 2316 20 -2.180357655574525 2316 21 -2.5519388610663043 2316 22 -2.3573193088315136 2316 23 -2.3293048903424136 2316 24 -2.2571647358257536 2316 25 -2.4314137660066706 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59363.0 3 0.0 4 504.0 5 2520.0 6 2935.0 7 2670.0 8 2462.0 9 2370.0 10 2332.0 11 2154.0 12 2223.0 13 2091.0 14 2180.0 15 2272.0 16 2375.0 17 2621.0 18 3026.0 19 3608.0 20 4679.0 21 5999.0 22 7344.0 23 8569.0 24 9935.0 25 11485.0 26 12749.0 27 14107.0 28 16535.0 29 19462.0 30 24139.0 31 30329.0 32 38951.0 33 50660.0 34 68267.0 35 95730.0 36 150456.0 37 191579.0 38 457163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.288912667459066 18.09203826593426 12.719744893771603 24.899304172835077 2 16.96889988063158 20.46735555666744 39.389116427493015 23.174628135207964 3 19.48802441626819 23.706685594948944 29.219877128291806 27.58541286049106 4 12.513641434698947 16.08366949273622 37.69785780077274 33.704831271792095 5 13.54028289067701 37.421837239064814 35.3916573697186 13.64622250053958 6 32.58782956034302 36.64370548484471 17.540377126771865 13.228087828040405 7 28.665860086758006 31.411094324251206 21.29598949419536 18.62705609479543 8 25.594979344055986 36.800866972072676 19.804855286796915 17.799298397074427 9 26.41392140709689 15.57745447028675 19.595559959995256 38.413064162621104 10 15.168135432467677 28.795738704588082 33.887375707150696 22.148750155793547 11 35.22682020057089 22.496207757150543 23.59512221813528 18.68184982414329 12 23.486674712199925 24.297257121664877 31.299150963184086 20.91691720295111 13 29.307273506585886 21.355875012539478 25.623782150467683 23.713069330406945 14 21.465766458637955 21.401245132401712 27.298828736537157 29.834159672423176 15 23.558111751849005 29.62950015351364 23.510157739063292 23.302230355574068 16 22.83097388444223 28.08532014433322 26.576098686470434 22.50760728475412 17 22.179376886621817 27.89365608689176 27.24943078358833 22.677536242898093 18 21.964457792869062 27.298220761731635 28.92052553342189 21.816795911977408 19 23.587370539364848 26.587726204626076 27.530163150039062 22.29474010597001 20 23.60188593784674 26.780378221126515 27.976264663592342 21.641471177434408 21 23.419774881615634 26.291094440741702 27.367440291038204 22.92169038660446 22 23.294235130320587 27.00549918226643 27.516050681241982 22.184215006171 23 23.050078467285033 26.74119475466149 27.465981677033973 22.742745101019505 24 23.07241587908597 26.427828830773255 27.962205246214594 22.53755004392618 25 23.21794984815829 26.543800024926966 27.839242341797355 22.399007785117384 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213.0 1 213.0 2 302.5 3 392.0 4 392.0 5 392.0 6 1051.0 7 1710.0 8 1710.0 9 1710.0 10 2472.5 11 3235.0 12 3235.0 13 3235.0 14 4790.0 15 6345.0 16 6345.0 17 6345.0 18 10674.5 19 15004.0 20 15004.0 21 15004.0 22 23233.0 23 31462.0 24 31462.0 25 31462.0 26 44030.0 27 56598.0 28 56598.0 29 56598.0 30 73053.5 31 89509.0 32 89509.0 33 89509.0 34 100845.0 35 112181.0 36 112181.0 37 112181.0 38 122931.0 39 133681.0 40 133681.0 41 133681.0 42 144708.5 43 155736.0 44 155736.0 45 155736.0 46 161943.5 47 168151.0 48 168151.0 49 168151.0 50 166495.5 51 164840.0 52 164840.0 53 164840.0 54 151437.0 55 138034.0 56 138034.0 57 138034.0 58 120462.5 59 102891.0 60 102891.0 61 102891.0 62 85676.0 63 68461.0 64 68461.0 65 68461.0 66 53391.0 67 38321.0 68 38321.0 69 38321.0 70 28225.0 71 18129.0 72 18129.0 73 18129.0 74 12690.0 75 7251.0 76 7251.0 77 7251.0 78 4941.0 79 2631.0 80 2631.0 81 2631.0 82 1692.5 83 754.0 84 754.0 85 754.0 86 488.0 87 222.0 88 222.0 89 222.0 90 144.5 91 67.0 92 67.0 93 67.0 94 42.5 95 18.0 96 18.0 97 18.0 98 13.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.310313380613508 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 2.2799055207152217E-4 22 9.119622082860887E-4 23 6.839716562145664E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1315844.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.04780594674405 #Duplication Level Percentage of deduplicated Percentage of total 1 83.9389085217186 62.99430918119439 2 11.320522035361003 16.991606818512253 3 2.689939651677765 6.056222069626956 4 0.9153554022397984 2.747816583983849 5 0.41330403473701816 1.550878049797505 6 0.19928187846686216 0.8973400646330223 7 0.11838377789636213 0.6219109953566043 8 0.08173672007644461 0.4907329205616283 9 0.05208508805634971 0.35179844230548085 >10 0.2424248235180736 3.1304695299047407 >50 0.01575335831472018 0.8209338592198699 >100 0.010561564497518777 1.6391771148090322 >500 0.0010253784938190039 0.5711407590100644 >1k 7.177649456733028E-4 1.135663611084685 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4498 0.3418338344059022 No Hit GGTATCAACGCAGAGTACTTTTTTT 2692 0.20458352205884586 No Hit TATCAACGCAGAGTACTTTTTTTTT 2607 0.19812378975015274 No Hit GTCCTACAGTGGACATTTCTAAATT 1321 0.10039183976216026 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.599685069050739E-5 7 0.0 0.0 0.0 0.0 7.599685069050739E-5 8 0.0 0.0 0.0 0.0 7.599685069050739E-5 9 0.0 0.0 0.0 0.0 7.599685069050739E-5 10 0.0 0.0 0.0 1.5199370138101477E-4 7.599685069050739E-5 11 0.0 0.0 0.0 1.5199370138101477E-4 7.599685069050739E-5 12 0.0 0.0 0.0 1.5199370138101477E-4 7.599685069050739E-5 13 0.0 0.0 0.0 2.2799055207152217E-4 7.599685069050739E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGCG 45 6.9990725E-4 14.710568 7 AATCCCG 150 0.0 13.240015 19 ATCGCGC 50 0.0015517204 13.239512 8 CGCGCCT 60 4.2516348E-4 12.609059 12 GGTCTAT 60 0.0043754224 11.530221 1 CCAACGA 75 2.1622327E-4 11.348584 19 ACTGTTC 425 0.0 11.348153 8 TAGGACC 275 0.0 11.348153 4 CTATACT 210 0.0 11.258088 4 GTGTAAG 185 1.8189894E-12 11.218594 1 GTCCTAA 415 0.0 11.192848 1 TCCAACG 110 5.2640644E-7 11.176637 18 GTTAGAT 60 0.0060775485 11.0329275 3 TCGCGCG 60 0.0060775485 11.0329275 9 TGGCGAG 680 0.0 10.986987 18 GGCGAGG 355 0.0 10.922346 19 GTATCAA 1800 0.0 10.816445 1 TGTAGGA 905 0.0 10.592879 2 GTCTAAA 140 5.423317E-8 10.588979 1 CTGTAGG 860 0.0 10.572562 1 >>END_MODULE