FastQCFastQC Report
Thu 26 May 2016
SRR1511735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511735_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315844
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53960.4100790063259778No Hit
GGTATCAACGCAGAGTACTTTTTTT33120.2517015694869605No Hit
TATCAACGCAGAGTACTTTTTTTTT33030.2510175978307459No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16820.1278267028614334No Hit
GTCCTACAGTGGACATTTCTAAATT15900.12083499259790674No Hit
CTGTAGGACGTGGAATATGGCAAGA14260.10837150908466354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG500.001502909313.2957977
ATCGCGC500.001502909313.2957978
TCGCGCG500.001502909313.2957979
GTATAGG1450.013.1603181
GCGTGCG604.102208E-412.6626639
GGTATCA9800.012.5593751
TAGGACC3300.012.3791094
GTCTAGT701.04743216E-412.2672971
GGCGTGC658.043283E-411.6886138
CCAACGA658.043283E-411.68861319
TTAACCT907.45585E-611.6114134
TGTAGGA9950.011.4575842
CTAGACT1001.9280433E-611.4002964
TGGCGAG7250.011.39639818
AAGGCGT1001.935994E-611.3963986
CTGTAGG9950.011.12344551
GTAGGAC9850.011.0916593
TACGTCT600.0058737111.0836214
GATATAC4150.011.0356411
ATAGCAC951.3592524E-511.0002863
Produced by FastQC (version 0.11.5)