FastQCFastQC Report
Thu 26 May 2016
SRR1511734_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511734_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295144
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82080.6337519225661394No Hit
GGTATCAACGCAGAGTACTTTTTTT48090.37131006281926954No Hit
TATCAACGCAGAGTACTTTTTTTTT47380.3658280469198792No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23610.1822963315276139No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17770.13720482046783986No Hit
GAGTACTTTTTTTTTTTTTTTTTTT16310.12593194270289634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCG402.8575235E-416.5500079
GGTATCA14000.012.9858881
GTATAAG1301.4078978E-912.1606961
TCCAACG1302.8048817E-911.639564518
TAGGACC1900.011.4481244
GTATAGG1653.8198777E-1111.3776221
GTAGAAC5050.010.8616723
CGTCTCT700.001548411110.808166510
CGTAGGA650.00795313510.640612
GAACAGT3650.010.62309556
TAGAACA5350.010.4293774
GTATCAA29650.010.3970881
AGTACTG2053.6379788E-1210.1491335
GTAATAC1502.618981E-810.0876233
AGTGCAA750.002748210.08762310
ACCCCTC750.002748210.0876238
TAGACTG2002.7284841E-119.9300045
CTTATAC2107.2759576E-129.9074873
AGAACAG4300.09.8970135
GTTTAAG1201.0663802E-59.8805661
Produced by FastQC (version 0.11.5)