FastQCFastQC Report
Thu 26 May 2016
SRR1511734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511734_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295144
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT107440.8295602651133773No Hit
GGTATCAACGCAGAGTACTTTTTTT65670.5070478649478359No Hit
TATCAACGCAGAGTACTTTTTTTTT64460.4977052744714101No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32710.2525587888296591No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21600.1667768217279314No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18740.14469433514728863No Hit
GTGGTATCAACGCAGAGTACATGGG14710.11357810405638293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15650.013.5995461
TCTAGAC1457.2759576E-1212.4480733
GTATAGC1950.011.7465541
TGGCGAG4500.011.60733718
GTCCTAC4500.011.4528911
CTGTGCG752.0795762E-411.3962949
TCCAACG855.3386935E-511.17283618
GTAGGAC5150.010.8833183
GTATCAA35500.010.37750151
CTAGACT1659.276846E-1010.3634674
GGCGAGG2200.010.36026719
CGTTTCC1201.5288515E-610.288321514
GGACGTG5550.010.2669326
TGGTATC5100.010.2449312
GTGGTAT5150.010.1927021
GAACAGT3450.010.1850936
TAGGACC2151.8189894E-1210.1626264
GTTCTAG1701.4370016E-910.1054911
AGTGTAT3600.010.02451810
AACAGTG4400.09.9285897
Produced by FastQC (version 0.11.5)