FastQCFastQC Report
Thu 26 May 2016
SRR1511733_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511733_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2076431
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50590.24363920592593732No Hit
GGTATCAACGCAGAGTACTTTTTTT31380.15112469424700364No Hit
TATCAACGCAGAGTACTTTTTTTTT31280.15064309866304249No Hit
GTCCTACAGTGGACATTTCTAAATT21230.1022427424749486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2100.012.15883618
TAGGACC3600.012.0837814
GGTATCA12200.011.7463151
GGCGAGG4900.011.38684719
TAGCCCG600.0060791811.0330175
TAGGACT4100.010.610154
GCCGGTT1252.385841E-710.59169711
TAAGGGT2150.010.5565084
GTCCTAC12750.010.3869511
TGGCGAG10550.010.30843918
CGGTTTC1606.6520442E-910.04792813
GCGAACC1054.316448E-59.907211
GGCGTGC1054.316448E-59.90728
ACAGCGC1054.316448E-59.90728
GTCCTAA7100.09.8831071
CGGTAGG800.0032577459.8831061
AGGACCG1151.0836988E-59.8680415
GTATCAA22850.09.8182281
CCAACGA1554.3604814E-89.76193319
ATAGGAC3650.09.5864183
Produced by FastQC (version 0.11.5)