FastQCFastQC Report
Thu 26 May 2016
SRR1511733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511733_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2076431
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66360.31958682951660805No Hit
TATCAACGCAGAGTACTTTTTTTTT42960.20689346286970287No Hit
GGTATCAACGCAGAGTACTTTTTTT41540.20005480557745478No Hit
GTCCTACAGTGGACATTTCTAAATT22980.11067066519426844No Hit
CTGTAGGACGTGGAATATGGCAAGA22690.10927403800078114No Hit
GTCCTAAAGTGTGTATTTCTCATTT21340.10277249761730585No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG400.005279583414.2501264
ACGTGTA604.1035723E-412.6628147
TAGGACC3700.012.3244334
ACCGTGC853.9585557E-612.2903788
GGTATCA13700.011.5597791
TCCAACG1850.011.2938618
CGTGCGC855.3411502E-511.17307110
TAAGCGA951.3652891E-510.996654513
TCGAACT1303.2823664E-810.95820419
AATCCCG2750.010.70583319
CGACTAA803.782443E-410.68424917
AGGACCG909.516832E-510.5556495
ATAGGAC2750.010.3637283
GTCCTAA7700.010.28371
CCAACGA1409.7346856E-810.17547619
GACGTGA6350.010.1701347
GGCCGTC750.002654902410.1302527
CCATGCG750.002654902410.1302529
GGCGAGG5350.010.11841619
GTATCAA26100.010.08859351
Produced by FastQC (version 0.11.5)