FastQCFastQC Report
Thu 26 May 2016
SRR1511732_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511732_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261554
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52810.23351200103999287No Hit
GTCCTACAGTGGACATTTCTAAATT31790.14056706140998623No Hit
GGTATCAACGCAGAGTACTTTTTTT31180.13786980103061877No Hit
TATCAACGCAGAGTACTTTTTTTTT30740.13592423616681273No Hit
CTGTAGGACGTGGAATATGGCAAGA29570.1307508023244194No Hit
GTCCTAAAGTGTGTATTTCTCATTT26800.11850258715909504No Hit
TTGTAGAACAGTGTATATCAATGAG24950.110322371254456No Hit
CTTTAGGACGTGAAATATGGCGAGG23770.10510472002879437No Hit
GATATACACTGTTCTACAAATCCCG23070.10200950320001204No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA455.007137E-415.3750941
AATACCG500.001552342713.2394895
CCAACGA2650.013.20380319
TCCAACG4050.013.07603718
CCAACGT1603.6379788E-1211.820971519
GGTATCA12200.011.5853311
GGCGAGG8000.011.58455319
TAGGACC5950.011.4434964
CGGTAGG1155.2910036E-711.1732051
ATGTCGA1303.495188E-810.91166617
TATGTCG1053.6625643E-610.80774516
GTCCTAC16750.010.6215991
CAGGACG1252.3865869E-710.5915914
TGGCGAG16350.010.58464918
GACGTGA9700.010.5291967
ACTATCG1803.8198777E-1110.50753111
AGGACGT18900.010.4574955
GTATTAG2650.010.443461
GGTCTAC2650.010.443461
TTAGGAC12050.010.4377713
Produced by FastQC (version 0.11.5)