FastQCFastQC Report
Thu 26 May 2016
SRR1511732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511732_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261554
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69640.3079298570805738No Hit
TATCAACGCAGAGTACTTTTTTTTT42510.18796809627362424No Hit
GGTATCAACGCAGAGTACTTTTTTT41650.1841654013125488No Hit
GTCCTACAGTGGACATTTCTAAATT36670.16214514444492592No Hit
CTGTAGGACGTGGAATATGGCAAGA33870.1497642771297966No Hit
GTCCTAAAGTGTGTATTTCTCATTT29210.12915897652675992No Hit
TTGTAGAACAGTGTATATCAATGAG28460.125842672781636No Hit
CTTTAGGACGTGAAATATGGCGAGG26050.11518628341397111No Hit
GATATACACTGTTCTACAAATCCCG25680.11355024023304329No Hit
GTGTATATCAATGAGTTACAATGAA23310.10307072039845168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4450.015.36579718
CCAACGA3300.013.23812419
TAGGACC5900.012.3994894
GGTATCA13300.011.9067321
CGTTTCC2650.011.82634714
ATAGGAC2250.011.823353
GCCGGTT3700.011.80697611
CCGTTTC2700.011.60734213
CCAACGT2150.011.48464219
CGGTTTC3900.011.2014913
TGTAGAA12600.011.0843912
TGCCGGT3900.010.95797910
CCGGTTT4000.010.921452512
GAACAGT8000.010.684036
TTGTAGA7750.010.5860241
ATCCCGT2700.010.55212910
ACCGTGC909.549932E-510.5521298
TTCCAAC7300.010.53767417
GTAGAAC12000.010.5301713
GTCCTAC17550.010.4909861
Produced by FastQC (version 0.11.5)