FastQCFastQC Report
Thu 26 May 2016
SRR1511731_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511731_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986761
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46860.23586128376790164No Hit
GTCCTACAGTGGACATTTCTAAATT32100.16156950936725656No Hit
TATCAACGCAGAGTACTTTTTTTTT29110.14651988840127222No Hit
GGTATCAACGCAGAGTACTTTTTTT29080.1463688888598075No Hit
GTCCTAAAGTGTGTATTTCTCATTT27860.140228240840242No Hit
CTGTAGGACGTGGAATATGGCAAGA27610.13896991132803593No Hit
CTTTAGGACGTGAAATATGGCGAGG24280.12220896222545137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA250.004796073319.7691691
CGGTAGG851.0177246E-815.11761
CGTTAGG500.001111732813.8384181
TATACCG604.253638E-412.6089515
TCCAACG2300.012.33484418
TAGGACC5900.012.1815294
GGTATCA10900.012.0610081
TAATACT1800.011.5582064
CGTGCGC907.852494E-611.55820610
CGCCACC1002.039258E-611.34805514
CGTAGGA700.001070741311.2966682
CTATGCG600.006079784611.0328329
GCGTGCG951.4312354E-510.9498789
CGCCCTA951.4312354E-510.94987816
GGCGAGG8200.010.84062219
GTCCTAA10150.010.8097441
CCAACGT1408.971256E-910.80767219
GTCCTAT2103.6379788E-1210.355281
CTTACAC2053.6379788E-1210.1486673
ACTGTTC8700.010.10898
Produced by FastQC (version 0.11.5)