##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511731_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1986761 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.495122966476593 31.0 30.0 34.0 25.0 34.0 2 26.826991268703182 31.0 26.0 34.0 10.0 34.0 3 28.503856276623107 31.0 28.0 34.0 16.0 34.0 4 33.021295968664575 35.0 33.0 37.0 27.0 37.0 5 33.476560592844336 35.0 35.0 37.0 28.0 37.0 6 33.72931167865687 37.0 35.0 37.0 29.0 37.0 7 33.77281766654369 37.0 35.0 37.0 29.0 37.0 8 33.79942982573143 37.0 35.0 37.0 29.0 37.0 9 35.40188175628573 39.0 35.0 39.0 30.0 39.0 10 35.27185303113963 39.0 35.0 39.0 29.0 39.0 11 35.287183511252735 39.0 35.0 39.0 29.0 39.0 12 35.19007922945941 39.0 35.0 39.0 29.0 39.0 13 35.189219538736666 39.0 35.0 39.0 29.0 39.0 14 36.33275114621235 40.0 36.0 41.0 29.0 41.0 15 36.27445978655712 40.0 36.0 41.0 29.0 41.0 16 36.2114496912311 40.0 36.0 41.0 28.0 41.0 17 36.17982636059395 40.0 36.0 41.0 28.0 41.0 18 36.120532363983386 40.0 36.0 41.0 28.0 41.0 19 36.14756329523279 40.0 36.0 41.0 28.0 41.0 20 36.075427794284266 40.0 36.0 41.0 27.0 41.0 21 35.99078701464343 40.0 36.0 41.0 27.0 41.0 22 35.887446955119415 40.0 36.0 41.0 27.0 41.0 23 35.78078188569234 40.0 36.0 41.0 27.0 41.0 24 35.67156291068729 40.0 36.0 41.0 27.0 41.0 25 35.562624794829375 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.369712841692209 1101 2 3.868377996700474 1101 3 2.532767977960475 1101 4 1.4601049001717996 1101 5 1.1026768165445944 1101 6 0.8175091319329368 1101 7 0.6326959030585897 1101 8 0.33742849763464733 1101 9 0.6366210815464157 1101 10 0.7169293157856771 1101 11 0.6373988793115899 1101 12 0.6170721889253414 1101 13 0.7014003946360461 1101 14 0.7637110859986862 1101 15 0.6894630936893265 1101 16 0.7971012423161667 1101 17 0.8398926041287638 1101 18 0.7665453342839044 1101 19 0.9166183875956833 1101 20 0.8463184853290855 1101 21 0.7431346003394026 1101 22 0.8452791074707093 1101 23 0.9397137071427082 1101 24 0.9255532859939919 1101 25 0.7965096426727669 1102 1 1.518392612564778 1102 2 4.139281951502731 1102 3 2.683754716817976 1102 4 1.6012713314335656 1102 5 1.3077596005998373 1102 6 0.9896685795161133 1102 7 0.889383363824436 1102 8 0.931849401589453 1102 9 1.0428710815464157 1102 10 1.0117798336764494 1102 11 0.9999028027265311 1102 12 1.0313102617439256 1102 13 1.1339863174232434 1102 14 1.1911200627720717 1102 15 1.2994560315424337 1102 16 1.1722915279407715 1102 17 1.212052051711936 1102 18 1.2362683411891169 1102 19 1.244590374413015 1102 20 1.1748986014621678 1102 21 1.2555345532584283 1102 22 1.0231608557444076 1102 23 1.1314568804007124 1102 24 1.0403955647133856 1102 25 0.977184862384 1103 1 1.3987833714900937 1103 2 3.974145429865551 1103 3 2.5405339025362004 1103 4 1.5287499350692855 1103 5 1.1108867493461574 1103 6 0.8185737130592088 1103 7 0.7629542197740733 1103 8 0.6760081386890491 1103 9 0.8434880192917902 1103 10 0.6592910111181354 1103 11 0.6894614403302626 1103 12 0.8839321679453036 1103 13 0.9039781739717512 1103 14 1.0184484720106113 1103 15 0.7804789377723296 1103 16 0.7392636532548664 1103 17 1.0078108027536317 1103 18 1.2263580459018613 1103 19 1.0509435340405062 1103 20 1.1208327377334797 1103 21 1.0540757030377321 1103 22 1.08043371840526 1103 23 0.8454797771246376 1103 24 0.9807290976721887 1103 25 0.6990112135790625 1104 1 1.4001363902407569 1104 2 3.8690086273311017 1104 3 2.4276209559384547 1104 4 1.5157223926642942 1104 5 1.121899163891939 1104 6 0.8691707935945985 1104 7 0.734794877032563 1104 8 0.804523092229239 1104 9 0.8623305410058748 1104 10 0.8833388503202144 1104 11 1.0248776080403204 1104 12 0.8674012680044214 1104 13 0.8353850042456585 1104 14 0.9638487236363247 1104 15 1.145780661256893 1104 16 0.9401461622360827 1104 17 1.0077704820066415 1104 18 0.7914615004500689 1104 19 0.8594017960040929 1104 20 0.7964805223661244 1104 21 0.8437220628018665 1104 22 0.9186868902534968 1104 23 0.9782854038394078 1104 24 1.085309291999998 1104 25 1.0468443523824718 1105 1 1.5891239537873005 1105 2 3.9995327965587784 1105 3 2.5670775432377617 1105 4 1.710106745135782 1105 5 1.255167394928499 1105 6 1.0259654628653507 1105 7 0.9859911317357231 1105 8 0.888504480631461 1105 9 0.9440400507281339 1105 10 0.715997608972593 1105 11 0.8320413498905666 1105 12 0.9757713171183653 1105 13 0.8364425336134929 1105 14 0.8846201858530591 1105 15 1.1316456122865617 1105 16 0.9834198503769329 1105 17 1.0685402209272823 1105 18 1.0732238579191247 1105 19 1.066036485954406 1105 20 1.211613359773139 1105 21 1.2028276721306455 1105 22 1.2574926460624383 1105 23 1.3487182383742606 1105 24 1.2866902266009106 1105 25 1.2142826727432166 1106 1 1.0144074354795443 1106 2 3.531759119759016 1106 3 2.0506704570524725 1106 4 1.1566588129555484 1106 5 0.5493427364968042 1106 6 0.4641638511683013 1106 7 0.3509045350777029 1106 8 0.21205514040644857 1106 9 0.4000992774842871 1106 10 0.1749452954750481 1106 11 0.2808800621072933 1106 12 0.24091478151793666 1106 13 0.3142102811354519 1106 14 0.3274058291289137 1106 15 0.5112596886714087 1106 16 0.3126515708705284 1106 17 0.27328567461860587 1106 18 0.3484972458729132 1106 19 0.35828132069006813 1106 20 0.29645662750351676 1106 21 0.3893599587623413 1106 22 0.4151155770047623 1106 23 0.43148118026098814 1106 24 0.3986342298410648 1106 25 0.21491242045054548 1107 1 1.366525782313797 1107 2 3.789781035127639 1107 3 2.459689646613043 1107 4 1.413066063412785 1107 5 1.013088208164092 1107 6 1.0081505776682107 1107 7 0.8513878775397714 1107 8 0.7526094627821038 1107 9 0.9133804649244297 1107 10 0.7700673482551466 1107 11 0.8368955777526565 1107 12 0.750030063019274 1107 13 0.7005802799503158 1107 14 0.8793041254188054 1107 15 0.8952104127241824 1107 16 0.9859674795319364 1107 17 0.953054157102649 1107 18 0.9464908461496577 1107 19 0.9706835871786481 1107 20 1.0005555893902525 1107 21 1.0980543575631145 1107 22 1.052575738887647 1107 23 1.0723423562341594 1107 24 1.0940724251062903 1107 25 1.1169479680624974 1108 1 1.4895248545463495 1108 2 3.9728062572060807 1108 3 2.5932644598140016 1108 4 1.528345140977649 1108 5 1.1523537772194814 1108 6 0.9770836989660623 1108 7 0.8767295289119659 1108 8 0.9249159411303793 1108 9 1.0370950895825786 1108 10 0.9398116264614984 1108 11 0.9578193594351134 1108 12 0.8936018734315496 1108 13 0.9899497347219892 1108 14 0.9209801393312773 1108 15 1.0434466446687338 1108 16 0.9937340645532728 1108 17 1.0720437658379325 1108 18 0.9949870953568833 1108 19 0.9881215755526043 1108 20 1.0670179407488476 1108 21 0.999171314162723 1108 22 1.1512360642391286 1108 23 1.2287687953172721 1108 24 1.1998379140424333 1108 25 1.2028395741235514 1109 1 1.3324718094477674 1109 2 3.458404438610902 1109 3 2.213803539116576 1109 4 1.4417238130236711 1109 5 1.0318738386162423 1109 6 0.840843199763782 1109 7 0.7475687317003192 1109 8 0.7156201188542823 1109 9 0.7144129754482478 1109 10 0.7493783255912163 1109 11 0.7263172628661891 1109 12 0.752813641210551 1109 13 0.7398435927300255 1109 14 0.864597762213819 1109 15 0.6205333713293868 1109 16 0.6826111557372698 1109 17 0.7281250358663982 1109 18 0.8025890782013505 1109 19 0.9990901209180052 1109 20 0.8577993126487229 1109 21 0.8976657040137326 1109 22 1.0470739783827696 1109 23 0.9033724343503309 1109 24 0.8875344323609795 1109 25 0.9485836226393474 1110 1 1.1217029082150418 1110 2 3.6468569611017685 1110 3 2.200588680945014 1110 4 1.3995297922969314 1110 5 0.7568243803705528 1110 6 0.6404145616344792 1110 7 0.6358838300632641 1110 8 0.52420034229484 1110 9 0.4817060330027232 1110 10 0.5190092780294719 1110 11 0.5640956610447816 1110 12 0.7083905096732792 1110 13 0.5811367754343237 1110 14 0.5456645200152295 1110 15 0.5878247441747604 1110 16 0.5306289751460795 1110 17 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-1.4943583654704682 2315 20 -1.6862933799728168 2315 21 -1.8505591203098462 2315 22 -1.7593465802297104 2315 23 -1.6136084843039669 2315 24 -1.72359554225568 2315 25 -1.661970206945007 2316 1 -0.3604293947166717 2316 2 1.9988231694438845 2316 3 0.5874077070484311 2316 4 -0.34150404311191807 2316 5 -0.46939040507071184 2316 6 -0.6753709870225606 2316 7 -1.098072689427795 2316 8 -0.9959133704522003 2316 9 -1.371098603009095 2316 10 -1.3207181932042644 2316 11 -1.3815572824011255 2316 12 -1.5712523630949278 2316 13 -1.4655514750439593 2316 14 -1.6926943413459057 2316 15 -1.6383866605097737 2316 16 -1.8511704226814842 2316 17 -1.663024384961929 2316 18 -1.8569975936296714 2316 19 -1.7393293398839447 2316 20 -1.5695006328981123 2316 21 -1.84927615458799 2316 22 -1.7785329022551153 2316 23 -1.7696871816096262 2316 24 -1.7470037830578562 2316 25 -1.7184633969891863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60118.0 3 2.0 4 485.0 5 2781.0 6 3283.0 7 3076.0 8 2828.0 9 2760.0 10 2769.0 11 2543.0 12 2519.0 13 2606.0 14 2603.0 15 2742.0 16 2832.0 17 3136.0 18 3615.0 19 4438.0 20 5589.0 21 7237.0 22 8926.0 23 10851.0 24 12680.0 25 14511.0 26 16714.0 27 19190.0 28 22200.0 29 27177.0 30 34533.0 31 44203.0 32 57655.0 33 76149.0 34 105064.0 35 150948.0 36 237765.0 37 304570.0 38 725663.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.068592548373964 18.78308462869968 12.933010060092784 25.215312762833577 2 16.379914908303096 21.227574255316124 39.838107969757566 22.55440286662321 3 18.84922242786123 24.59742263915992 29.706743790521354 26.846611142457498 4 12.271682401657774 17.092594428821585 38.51535237504662 32.12037079447402 5 13.229170494085599 37.59239284443373 35.92012325589238 13.258313405588293 6 31.312271581735295 37.267743830284566 18.507057466902157 12.912927121077974 7 27.556359320522194 31.47273376113181 22.57558911212773 18.395317806218262 8 24.595912643745272 37.27217315016753 21.02759214621185 17.104322059875347 9 26.29646947972101 15.526477517929937 20.37945178106476 37.797601221284296 10 14.948702939105408 29.185040374760728 34.544819432231655 21.321437253902207 11 34.30936081390767 22.51342763422475 24.685707037736297 18.491504514131293 12 23.043285025224474 24.48095165951013 32.277762649860755 20.19800066540465 13 29.092527989023342 21.851697310345834 25.903619006010288 23.15215569462054 14 20.81830678174174 22.191345612280493 27.85433174901259 29.13601585696518 15 23.01635677366326 30.650742590578332 23.672500114507987 22.660400521250416 16 22.07995828386001 28.032662207482428 27.972312724077025 21.91506678458053 17 21.614426697524262 28.193929717766757 28.175155441444645 22.016488143264336 18 21.477822445679173 27.601709516142105 29.90505652164503 21.015411516533693 19 23.44413847463283 26.706181568895303 27.56456362894178 22.285116327530087 20 23.292283269099805 27.444921658921228 28.273607142479644 20.98918792949932 21 22.963715725534488 26.74188476088659 27.342512115227034 22.951887398351886 22 22.899001684160936 27.86319942257339 27.704397039178634 21.533401854087035 23 22.643490480945786 27.3974809135715 27.76129079019425 22.19773781528847 24 22.932703027691804 26.80065694867173 28.501213784647472 21.76542623898899 25 22.975486231106814 27.167686500791994 28.11848028021488 21.738346987886313 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 279.0 1 279.0 2 420.5 3 562.0 4 562.0 5 562.0 6 1374.5 7 2187.0 8 2187.0 9 2187.0 10 3004.5 11 3822.0 12 3822.0 13 3822.0 14 6416.5 15 9011.0 16 9011.0 17 9011.0 18 16038.5 19 23066.0 20 23066.0 21 23066.0 22 37450.0 23 51834.0 24 51834.0 25 51834.0 26 74912.0 27 97990.0 28 97990.0 29 97990.0 30 127663.5 31 157337.0 32 157337.0 33 157337.0 34 175566.5 35 193796.0 36 193796.0 37 193796.0 38 206466.0 39 219136.0 40 219136.0 41 219136.0 42 230969.5 43 242803.0 44 242803.0 45 242803.0 46 247581.0 47 252359.0 48 252359.0 49 252359.0 50 243824.5 51 235290.0 52 235290.0 53 235290.0 54 211133.5 55 186977.0 56 186977.0 57 186977.0 58 161212.0 59 135447.0 60 135447.0 61 135447.0 62 112161.5 63 88876.0 64 88876.0 65 88876.0 66 68979.5 67 49083.0 68 49083.0 69 49083.0 70 35888.0 71 22693.0 72 22693.0 73 22693.0 74 15885.5 75 9078.0 76 9078.0 77 9078.0 78 6272.5 79 3467.0 80 3467.0 81 3467.0 82 2320.5 83 1174.0 84 1174.0 85 1174.0 86 768.0 87 362.0 88 362.0 89 362.0 90 230.0 91 98.0 92 98.0 93 98.0 94 62.5 95 27.0 96 27.0 97 27.0 98 17.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.328049523822946 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 2.516659024412096E-4 22 7.549977073236287E-4 23 4.529986243941773E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1986761.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.47771882073255 #Duplication Level Percentage of deduplicated Percentage of total 1 83.10406980944084 60.23193404507183 2 11.839124695838816 17.161455015771928 3 2.822272717023571 6.136556652595778 4 0.9993503035719429 2.8972252122280393 5 0.4351171321475272 1.5768148578935999 6 0.2118982304643124 0.9214740219721931 7 0.12381493827928532 0.6281676997688678 8 0.09768554842683802 0.5664020569383534 9 0.06106027064222151 0.39829582140522857 >10 0.267158343171143 3.422137066399733 >50 0.019183472874094883 0.9534490058346871 >100 0.01603172083718446 2.2980183613478724 >500 0.001967801823996603 0.988368985951232 >1k 0.0012650154582835168 1.8197011968206285 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4686 0.23586128376790164 No Hit GTCCTACAGTGGACATTTCTAAATT 3210 0.16156950936725656 No Hit TATCAACGCAGAGTACTTTTTTTTT 2911 0.14651988840127222 No Hit GGTATCAACGCAGAGTACTTTTTTT 2908 0.1463688888598075 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2786 0.140228240840242 No Hit CTGTAGGACGTGGAATATGGCAAGA 2761 0.13896991132803593 No Hit CTTTAGGACGTGAAATATGGCGAGG 2428 0.12220896222545137 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.033318048824192E-5 0.0 0.0 0.0 0.0 3 5.033318048824192E-5 0.0 0.0 0.0 0.0 4 5.033318048824192E-5 0.0 0.0 0.0 0.0 5 5.033318048824192E-5 0.0 0.0 0.0 0.0 6 5.033318048824192E-5 0.0 0.0 0.0 0.0 7 5.033318048824192E-5 0.0 0.0 0.0 0.0 8 5.033318048824192E-5 0.0 0.0 0.0 0.0 9 5.033318048824192E-5 0.0 0.0 5.033318048824192E-5 0.0 10 5.033318048824192E-5 0.0 0.0 5.033318048824192E-5 0.0 11 5.033318048824192E-5 0.0 0.0 1.0066636097648384E-4 0.0 12 5.033318048824192E-5 0.0 0.0 1.0066636097648384E-4 0.0 13 5.033318048824192E-5 0.0 0.0 1.0066636097648384E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTAA 25 0.0047960733 19.769169 1 CGGTAGG 85 1.0177246E-8 15.1176 1 CGTTAGG 50 0.0011117328 13.838418 1 TATACCG 60 4.253638E-4 12.608951 5 TCCAACG 230 0.0 12.334844 18 TAGGACC 590 0.0 12.181529 4 GGTATCA 1090 0.0 12.061008 1 TAATACT 180 0.0 11.558206 4 CGTGCGC 90 7.852494E-6 11.558206 10 CGCCACC 100 2.039258E-6 11.348055 14 CGTAGGA 70 0.0010707413 11.296668 2 CTATGCG 60 0.0060797846 11.032832 9 GCGTGCG 95 1.4312354E-5 10.949878 9 CGCCCTA 95 1.4312354E-5 10.949878 16 GGCGAGG 820 0.0 10.840622 19 GTCCTAA 1015 0.0 10.809744 1 CCAACGT 140 8.971256E-9 10.807672 19 GTCCTAT 210 3.6379788E-12 10.35528 1 CTTACAC 205 3.6379788E-12 10.148667 3 ACTGTTC 870 0.0 10.1089 8 >>END_MODULE