FastQCFastQC Report
Thu 26 May 2016
SRR1511731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511731_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986761
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59160.2977710957684392No Hit
GGTATCAACGCAGAGTACTTTTTTT37550.1890010927333484No Hit
TATCAACGCAGAGTACTTTTTTTTT36900.18572943600161268No Hit
GTCCTACAGTGGACATTTCTAAATT34490.17359913950394637No Hit
CTGTAGGACGTGGAATATGGCAAGA30550.15376786639157905No Hit
GTCCTAAAGTGTGTATTTCTCATTT29430.14813055017689594No Hit
CTTTAGGACGTGAAATATGGCGAGG27840.1401275744792655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6300.012.36440854
GGTATCA10300.012.1316451
GGCGAGG7000.011.80501619
TCCAACG2800.011.532474518
CCAACGA1900.011.49792919
AACGATC600.0058889311.0802227
GTATCAA22200.010.5698231
CTATACT2250.010.5549834
CATACGT1002.4040704E-510.44811719
GACGTGA8750.010.311397
GTCCTAC16300.010.2993711
TGGCGAG16700.010.29351618
TAGGACG16600.010.2434194
TTAGGAC10700.010.2097253
AGGACGT16800.010.1780195
GTAGGAC16800.010.1780193
GCGTGCG856.611714E-410.0559999
TGTAGGA18450.010.0401052
GGACGTG16550.09.9851116
GGACCTG12000.09.8930556
Produced by FastQC (version 0.11.5)