FastQCFastQC Report
Thu 26 May 2016
SRR1511730_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511730_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507331
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40970.27180493202886424No Hit
TATCAACGCAGAGTACTTTTTTTTT24570.16300334830239677No Hit
GTCCTACAGTGGACATTTCTAAATT24340.16147747243306215No Hit
GGTATCAACGCAGAGTACTTTTTTT23760.15762961154517488No Hit
CTGTAGGACGTGGAATATGGCAAGA21510.14270256499733636No Hit
GTCCTAAAGTGTGTATTTCTCATTT20610.13673174637820093No Hit
CTTTAGGACGTGAAATATGGCGAGG17700.11742609950966311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG5550.012.94932219
GGTATCA6700.012.838841
TAGGACT1850.012.7789354
TCCAACG2200.012.46527118
GTCTAGG1403.4924597E-1012.0061061
TAGGACC5050.011.7971084
AAGTCGT1052.8866089E-711.7079397
ACACCGT658.3386747E-411.6386616
CCAACGT1407.3305273E-1011.48278619
CCGGTTT1850.011.24546312
TGGCGAG14000.011.14505718
CTAGGAC2050.011.0709223
GCGGGAC600.006080072411.032483
GCGTGCG600.006080072411.032489
TATGTCG600.006080072411.0324816
GCCGGTT1900.010.9495311
AAGGCGT951.4311068E-510.949536
GTAGGAC13450.010.8977243
GTATTAA2001.8189894E-1210.876121
CGGTTTC2000.010.87487313
Produced by FastQC (version 0.11.5)