##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511730_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1507331 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.03986184852564 31.0 30.0 34.0 25.0 34.0 2 27.350969362402818 31.0 27.0 34.0 10.0 34.0 3 29.048029264972325 31.0 28.0 34.0 16.0 34.0 4 33.60487975103013 35.0 33.0 37.0 28.0 37.0 5 34.06183048049831 37.0 35.0 37.0 30.0 37.0 6 34.32054538784116 37.0 35.0 37.0 31.0 37.0 7 34.38140925914746 37.0 35.0 37.0 31.0 37.0 8 34.41024963992647 37.0 35.0 37.0 31.0 37.0 9 36.074008296784186 39.0 37.0 39.0 32.0 39.0 10 35.95985022533206 39.0 37.0 39.0 32.0 39.0 11 35.98661541492877 39.0 37.0 39.0 32.0 39.0 12 35.89961196313219 39.0 37.0 39.0 31.0 39.0 13 35.900141375716416 39.0 37.0 39.0 31.0 39.0 14 37.11503644521343 40.0 38.0 41.0 31.0 41.0 15 37.0727146194167 40.0 38.0 41.0 31.0 41.0 16 37.01979989796534 40.0 37.0 41.0 31.0 41.0 17 36.99812582637788 40.0 37.0 41.0 31.0 41.0 18 36.94847117189257 40.0 37.0 41.0 31.0 41.0 19 36.98381377414781 40.0 38.0 41.0 31.0 41.0 20 36.91967524054106 40.0 37.0 41.0 31.0 41.0 21 36.848198570851395 40.0 37.0 41.0 31.0 41.0 22 36.755800816144564 40.0 37.0 41.0 31.0 41.0 23 36.665854414193035 40.0 37.0 41.0 31.0 41.0 24 36.57269504840012 40.0 37.0 41.0 30.0 41.0 25 36.47618406308899 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7319350926298078 1101 2 3.346064701805812 1101 3 2.0161860797646405 1101 4 0.8905382832931394 1101 5 0.47677226717317467 1101 6 0.27645457333770196 1101 7 0.14244630460275687 1101 8 0.0686329187026189 1101 9 0.016313500217719934 1101 10 0.14089842092528926 1101 11 0.1311245434876085 1101 12 0.036860603303196626 1101 13 0.13597224799220697 1101 14 0.16155592557150555 1101 15 0.17987443062745 1101 16 0.22486801610463658 1101 17 0.336706046800316 1101 18 0.02559684386729799 1101 19 0.24694537985784848 1101 20 0.16011255797854318 1101 21 0.07756277043542781 1101 22 0.025990201462548157 1101 23 0.08875125365829462 1101 24 -0.06129308688957735 1101 25 -0.20329729423198728 1102 1 1.4651311057979939 1102 2 4.0925116562082415 1102 3 2.693217235982246 1102 4 1.5330111375518882 1102 5 1.2437399469170884 1102 6 0.8382531946796234 1102 7 0.8344881008596445 1102 8 0.7368828595912902 1102 9 0.8607452129912261 1102 10 0.7757559171512582 1102 11 0.8625117499771235 1102 12 0.7660161297577943 1102 13 0.8447671043971425 1102 14 0.9593151514859102 1102 15 1.0255965891913164 1102 16 0.829631479846654 1102 17 0.9869825059407447 1102 18 0.9267899833154729 1102 19 0.889458247939551 1102 20 1.0059693084923609 1102 21 0.9035281039142617 1102 22 0.725008953395033 1102 23 0.9054552059327179 1102 24 0.9900191846223976 1102 25 0.7589105593901735 1103 1 1.3169128512904784 1103 2 3.9179751204527804 1103 3 2.412785093109445 1103 4 1.4095571508168945 1103 5 0.9931948366895327 1103 6 0.7571113345101494 1103 7 0.6970243209903657 1103 8 0.6141719060977167 1103 9 0.6151834667096807 1103 10 0.5313795171144378 1103 11 0.5982413472487238 1103 12 0.7152825214217913 1103 13 0.6404855671293177 1103 14 0.7904804643270182 1103 15 0.6153475525043248 1103 16 0.7040303681461779 1103 17 0.7122381838983003 1103 18 0.6461426019048915 1103 19 0.7633179439753874 1103 20 0.6619769185555313 1103 21 0.7285312820800485 1103 22 0.7633507837555058 1103 23 0.7623487571055705 1103 24 0.8670469639831708 1103 25 0.5093057478663638 1104 1 1.447874063590298 1104 2 3.9680807377386316 1104 3 2.5836034782437345 1104 4 1.7204950314708398 1104 5 1.2229866378495302 1104 6 1.0526885816377103 1104 7 0.8963063852203703 1104 8 0.8314070089516008 1104 9 0.9859058399678844 1104 10 0.8710939387822165 1104 11 0.9557829872278276 1104 12 0.8340885626545429 1104 13 0.8370253396478802 1104 14 1.104715428271902 1104 15 1.0579770788161866 1104 16 0.8236272195984142 1104 17 0.925114330689226 1104 18 0.9014497491319702 1104 19 1.0456080594387487 1104 20 1.162429745015963 1104 21 1.2330652465978744 1104 22 1.142307209816856 1104 23 1.2269039506345223 1104 24 1.1926234891216865 1104 25 1.0870789563307781 1105 1 1.302851802789629 1105 2 3.7656642419441653 1105 3 2.2894759916842773 1105 4 1.381743698325451 1105 5 0.9572187311348372 1105 6 0.6465405193990179 1105 7 0.7412918976251959 1105 8 0.6008837656981854 1105 9 0.6860740098678235 1105 10 0.38864439937779593 1105 11 0.40770782884395373 1105 12 0.5064862393345422 1105 13 0.617283194457066 1105 14 0.5048074917734695 1105 15 0.6913337560082624 1105 16 0.5026601421397245 1105 17 0.6886450344231463 1105 18 0.6470028296015471 1105 19 0.6151521525940069 1105 20 0.6613146729116721 1105 21 0.6912099360268726 1105 22 0.8253580710512338 1105 23 0.7914132140965648 1105 24 0.5718228703454429 1105 25 0.8322581357763141 1106 1 1.225499018687806 1106 2 3.837659578719805 1106 3 2.334749380727672 1106 4 1.3860231742669598 1106 5 0.8582797461940288 1106 6 0.6298279704499592 1106 7 0.47162948820179906 1106 8 0.5407310836161372 1106 9 0.5584093186209032 1106 10 0.49156131009499404 1106 11 0.559017434907652 1106 12 0.5834186380815609 1106 13 0.6011962916449889 1106 14 0.7775802831181338 1106 15 0.8553563788465084 1106 16 0.6953366065251032 1106 17 0.6933876388114086 1106 18 0.6233809302958164 1106 19 0.7222541286869983 1106 20 0.6677228487727263 1106 21 0.6431554277814442 1106 22 0.551943663623149 1106 23 0.689453091052826 1106 24 0.5145203539985559 1106 25 0.7483294913758272 1107 1 1.1930451437563931 1107 2 3.6767569070329493 1107 3 2.2749415519347487 1107 4 1.4512806964539067 1107 5 0.984657668712984 1107 6 0.7791849168080205 1107 7 0.5939387628128614 1107 8 0.5698510175850444 1107 9 0.7116845478004308 1107 10 0.589137609966464 1107 11 0.5639091581655649 1107 12 0.5472731846128482 1107 13 0.6166973127395963 1107 14 0.7027345576968145 1107 15 0.7855416378862259 1107 16 0.958880799517992 1107 17 0.7969822434345133 1107 18 0.687410548053883 1107 19 0.7788862468545261 1107 20 0.9985806330113149 1107 21 1.0404014796569356 1107 22 0.911685886972208 1107 23 0.8450166455169068 1107 24 1.0664032487439243 1107 25 0.8796791776448529 1108 1 1.4846633252864159 1108 2 4.036994173480565 1108 3 2.511641192665099 1108 4 1.4217400060589327 1108 5 1.0034160988706091 1108 6 0.929546396962273 1108 7 0.7908046835800207 1108 8 0.7409316042614549 1108 9 0.7642653751299449 1108 10 0.6581078732501382 1108 11 0.7813723991331543 1108 12 0.6555166171186144 1108 13 0.7045252703746385 1108 14 0.7240972131724845 1108 15 0.7071554567966416 1108 16 0.6503084605282723 1108 17 0.8310090080640933 1108 18 0.8629822138822263 1108 19 0.8179600535362539 1108 20 0.9271087452148947 1108 21 0.73110843257151 1108 22 0.9255332419985223 1108 23 0.8745264722550132 1108 24 0.9094869951914717 1108 25 0.9006239225233656 1109 1 1.10142596124291 1109 2 3.2148572572937475 1109 3 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-2.073121766798316 2315 15 -1.9233175413683625 2315 16 -2.077566368416761 2315 17 -1.801843444274816 2315 18 -2.012062565700795 2315 19 -2.023289174104683 2315 20 -2.0382757749853155 2315 21 -2.1252398821947764 2315 22 -1.990798820264068 2315 23 -2.0741811778220125 2315 24 -2.0227486653007816 2315 25 -2.124591800455427 2316 1 -0.3189974492828398 2316 2 2.2004734466832545 2316 3 0.6233235846485776 2316 4 -0.11514046342190909 2316 5 -0.45355693243218553 2316 6 -0.5756361974054229 2316 7 -0.8424536349167226 2316 8 -0.9368794619643097 2316 9 -1.1801625725087703 2316 10 -1.2878460640179128 2316 11 -1.1636662813133327 2316 12 -1.407887135813425 2316 13 -1.3174046705696085 2316 14 -1.4258402183043515 2316 15 -1.3743238812700866 2316 16 -1.7645425396204075 2316 17 -1.5655692876712664 2316 18 -1.7312241641057824 2316 19 -1.6781087225594078 2316 20 -1.377786341418613 2316 21 -1.6510839465781544 2316 22 -1.578442628580035 2316 23 -1.5755381180043457 2316 24 -1.5485225971043661 2316 25 -1.5839209572823094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35268.0 3 0.0 4 300.0 5 1706.0 6 2026.0 7 1855.0 8 1649.0 9 1651.0 10 1486.0 11 1489.0 12 1461.0 13 1469.0 14 1409.0 15 1534.0 16 1602.0 17 1719.0 18 1981.0 19 2477.0 20 3077.0 21 4042.0 22 4940.0 23 5800.0 24 6906.0 25 8026.0 26 9329.0 27 10859.0 28 12719.0 29 15945.0 30 20321.0 31 27289.0 32 36643.0 33 52133.0 34 75030.0 35 113556.0 36 187394.0 37 248672.0 38 603568.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57193675443549 18.703788351728985 13.097587722935442 25.626687170900087 2 15.899701167859313 21.026392677086484 40.629101311587306 22.444804843466898 3 18.736495169276026 24.30799870764948 29.79982498867203 27.155681134402464 4 11.809284092213321 17.000048429973244 39.09075047219224 32.099917005621194 5 12.848206531942886 37.62206177674313 36.341321182938586 13.1884105083754 6 30.900114175320482 37.843977202087665 18.530435584486753 12.7254730381051 7 27.265146142419944 31.517762190255493 22.68831464356535 18.52877702375921 8 24.081041257693233 37.81213283611894 21.024645548986918 17.082180357200908 9 25.71007960428068 15.656614240667777 20.518784527087945 38.1145216279636 10 14.187593833073162 29.417427227330954 35.08167748158832 21.313301458007565 11 34.03937157797458 22.553573170060194 24.927836022744838 18.47921922922039 12 22.612286219815026 24.605677187027933 32.7485469349466 20.033489658210442 13 28.69442743498276 22.07106468320495 26.146281075623072 23.08822680618922 14 20.36918234946405 22.282099950176836 28.28794737187784 29.06077032848127 15 22.520202928222137 31.01899980827038 23.960430721586697 22.500366541920787 16 21.541585756545842 28.373529105418783 28.340888630300842 21.74399650773453 17 21.150563479421574 28.41333456287969 28.528505019799898 21.907596937898845 18 20.876436562374156 27.778238489090985 30.412430978995324 20.93289396953954 19 22.775238335334663 26.942076386723546 28.113021037198227 22.16966424074357 20 22.630464045388837 27.69119722210981 28.86943876295253 20.808899969548825 21 22.44934244526901 26.99872025114657 27.78056785289456 22.771369450689864 22 22.079746941255927 28.240291696908017 28.26138877131435 21.41857259052171 23 21.924763305542342 27.681857173280044 28.280932487595557 22.112447033582054 24 22.350049425142473 27.02971479370808 29.01919287747209 21.601042903677364 25 22.214563357351505 27.459529459687353 28.698076268583346 21.627830914377796 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 191.0 1 191.0 2 294.0 3 397.0 4 397.0 5 397.0 6 1100.5 7 1804.0 8 1804.0 9 1804.0 10 2689.5 11 3575.0 12 3575.0 13 3575.0 14 5551.0 15 7527.0 16 7527.0 17 7527.0 18 13578.0 19 19629.0 20 19629.0 21 19629.0 22 30632.0 23 41635.0 24 41635.0 25 41635.0 26 59733.5 27 77832.0 28 77832.0 29 77832.0 30 100660.0 31 123488.0 32 123488.0 33 123488.0 34 137679.0 35 151870.0 36 151870.0 37 151870.0 38 161915.0 39 171960.0 40 171960.0 41 171960.0 42 179344.0 43 186728.0 44 186728.0 45 186728.0 46 190050.5 47 193373.0 48 193373.0 49 193373.0 50 184791.0 51 176209.0 52 176209.0 53 176209.0 54 156255.0 55 136301.0 56 136301.0 57 136301.0 58 116950.5 59 97600.0 60 97600.0 61 97600.0 62 79170.0 63 60740.0 64 60740.0 65 60740.0 66 46487.5 67 32235.0 68 32235.0 69 32235.0 70 23639.5 71 15044.0 72 15044.0 73 15044.0 74 10534.5 75 6025.0 76 6025.0 77 6025.0 78 4080.0 79 2135.0 80 2135.0 81 2135.0 82 1433.0 83 731.0 84 731.0 85 731.0 86 468.0 87 205.0 88 205.0 89 205.0 90 137.0 91 69.0 92 69.0 93 69.0 94 45.0 95 21.0 96 21.0 97 21.0 98 14.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.359958098121779 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 6.634242910150458E-5 20 0.0 21 2.6536971640601833E-4 22 8.624515783195595E-4 23 5.307394328120367E-4 24 6.634242910150458E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1507331.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.05626023059337 #Duplication Level Percentage of deduplicated Percentage of total 1 86.50649414384584 66.65866924384486 2 9.913969441233125 15.2786681836362 3 2.1139650373548515 4.886827201103745 4 0.6466937533552072 1.9932720859215203 5 0.2657287285326846 1.0238031028279624 6 0.13895686321157313 0.6424497727474768 7 0.07876100708504086 0.4248320060577961 8 0.05602167100706725 0.34534563677386093 9 0.042542792767425605 0.2950369659380658 >10 0.20393848190216635 2.893188955270784 >50 0.016263085965496145 0.897233475651229 >100 0.013887633951777863 2.136256765906369 >500 0.0017358498673570606 0.9629914344726576 >1k 0.0010415099204142363 1.5614251698474295 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4097 0.27180493202886424 No Hit TATCAACGCAGAGTACTTTTTTTTT 2457 0.16300334830239677 No Hit GTCCTACAGTGGACATTTCTAAATT 2434 0.16147747243306215 No Hit GGTATCAACGCAGAGTACTTTTTTT 2376 0.15762961154517488 No Hit CTGTAGGACGTGGAATATGGCAAGA 2151 0.14270256499733636 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2061 0.13673174637820093 No Hit CTTTAGGACGTGAAATATGGCGAGG 1770 0.11742609950966311 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAGG 555 0.0 12.949322 19 GGTATCA 670 0.0 12.83884 1 TAGGACT 185 0.0 12.778935 4 TCCAACG 220 0.0 12.465271 18 GTCTAGG 140 3.4924597E-10 12.006106 1 TAGGACC 505 0.0 11.797108 4 AAGTCGT 105 2.8866089E-7 11.707939 7 ACACCGT 65 8.3386747E-4 11.638661 6 CCAACGT 140 7.3305273E-10 11.482786 19 CCGGTTT 185 0.0 11.245463 12 TGGCGAG 1400 0.0 11.145057 18 CTAGGAC 205 0.0 11.070922 3 GCGGGAC 60 0.0060800724 11.03248 3 GCGTGCG 60 0.0060800724 11.03248 9 TATGTCG 60 0.0060800724 11.03248 16 GCCGGTT 190 0.0 10.94953 11 AAGGCGT 95 1.4311068E-5 10.94953 6 GTAGGAC 1345 0.0 10.897724 3 GTATTAA 200 1.8189894E-12 10.87612 1 CGGTTTC 200 0.0 10.874873 13 >>END_MODULE