FastQCFastQC Report
Thu 26 May 2016
SRR1511730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507331
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53240.3532070925364104No Hit
TATCAACGCAGAGTACTTTTTTTTT36110.23956251148553304No Hit
GGTATCAACGCAGAGTACTTTTTTT32650.21660803101641243No Hit
GTCCTACAGTGGACATTTCTAAATT23250.15424614766099815No Hit
CTGTAGGACGTGGAATATGGCAAGA23190.15384809308638914No Hit
GTCCTAAAGTGTGTATTTCTCATTT20110.13341462492312572No Hit
CTTTAGGACGTGAAATATGGCGAGG19120.12684672444207676No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17820.11822220865888115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCC350.002173603516.2818479
GACCGTT500.00150219613.2968447
CGTGCGC655.464437E-513.150722510
TCCAACG1900.012.99691418
CCAACGT1051.999797E-812.66365919
GGTATCA9200.012.2256881
ACTATCG802.8731998E-511.87218111
CGGTTTC1459.822543E-1111.79030313
GCGTGCG658.0391753E-411.6895319
ACCGTGC600.005885913511.0807028
GGCGAGG6000.011.08070219
TAGGACC4300.011.0464534
GCCGGTT1252.2465065E-710.63747411
GACTGTG3250.010.228347
ATAACCG750.002647606410.133284
GTATCGC750.002652712210.1309276
GTGCGCC750.002652712210.13092711
GCGCCAC856.607107E-410.05643513
ATCGGTC856.607107E-410.05643514
CCTATAC951.6438343E-49.9999483
Produced by FastQC (version 0.11.5)