##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511730_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1507331 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.65864829954403 31.0 30.0 34.0 26.0 34.0 2 29.856089339368726 31.0 30.0 34.0 26.0 34.0 3 29.9298103734349 31.0 30.0 34.0 26.0 34.0 4 33.52687100577113 35.0 35.0 37.0 28.0 37.0 5 33.034376656487524 35.0 33.0 37.0 26.0 37.0 6 32.97831929416963 35.0 33.0 37.0 26.0 37.0 7 32.73387995072084 35.0 33.0 37.0 26.0 37.0 8 32.580705233289834 35.0 33.0 37.0 25.0 37.0 9 33.87830476517765 37.0 33.0 39.0 25.0 39.0 10 33.57907453638252 37.0 33.0 39.0 25.0 39.0 11 33.62540676201843 37.0 33.0 39.0 25.0 39.0 12 33.34405382759328 37.0 33.0 39.0 23.0 39.0 13 33.33280347846624 37.0 33.0 39.0 22.0 39.0 14 34.04756486796862 38.0 33.0 40.0 20.0 41.0 15 33.9409817750713 38.0 33.0 40.0 19.0 41.0 16 33.73291997577174 38.0 33.0 40.0 18.0 41.0 17 33.67235000142636 38.0 33.0 40.0 17.0 41.0 18 33.55364216618646 38.0 33.0 40.0 17.0 41.0 19 33.569385888036535 38.0 32.0 40.0 18.0 41.0 20 33.66732257214905 38.0 33.0 40.0 18.0 41.0 21 33.57834344281382 38.0 33.0 40.0 18.0 41.0 22 33.42211631021985 38.0 33.0 40.0 11.0 41.0 23 33.268832127780826 38.0 33.0 40.0 10.0 41.0 24 33.02299229565371 38.0 32.0 40.0 2.0 41.0 25 32.394446873314486 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19691738236783962 1101 2 0.260414243421323 1101 3 0.27582947161981863 1101 4 0.35274678974617757 1101 5 0.4209121292012412 1101 6 0.25337684597117516 1101 7 0.2782050462920509 1101 8 0.03278665429552774 1101 9 0.11212541491045869 1101 10 0.25173724542628406 1101 11 0.3391829300228437 1101 12 0.41500175155927366 1101 13 0.3621730881402101 1101 14 0.10853092447794666 1101 15 0.12418518325477379 1101 16 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0.7311936790829243 1103 4 0.9293311643423721 1103 5 0.9623900586768173 1103 6 0.9887513731325157 1103 7 0.8496335545026668 1103 8 0.886256232426966 1103 9 1.1418140184588026 1103 10 1.0896493916933991 1103 11 0.9295165766643407 1103 12 0.9027384806574617 1103 13 0.8850751182708976 1103 14 1.0136963494176356 1103 15 0.9565281441762181 1103 16 1.023277035328313 1103 17 1.1485980169179584 1103 18 1.0250913194967026 1103 19 1.1206155310597765 1103 20 1.0254989374180923 1103 21 1.2718584459140345 1103 22 1.4863395007840623 1103 23 1.2644706921544682 1103 24 1.2631915954394515 1103 25 1.2648504857043505 1104 1 0.4418621395077196 1104 2 0.576346119611447 1104 3 0.6483574696858376 1104 4 0.8084710431748832 1104 5 0.7279720388436459 1104 6 0.8807958561018054 1104 7 1.0352227376712904 1104 8 1.1346259516154547 1104 9 1.0920064855375813 1104 10 1.1243926865896157 1104 11 1.1665303793215145 1104 12 1.1702050211097443 1104 13 1.2104304023229204 1104 14 1.375962162138741 1104 15 1.3598261563419456 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1107 16 1.0674633729641485 1107 17 1.0707735342788567 1107 18 0.6870075453957014 1107 19 0.9772158051007409 1107 20 0.7947688796889523 1107 21 0.7800336890636501 1107 22 0.8118806635591156 1107 23 0.9544859100289571 1107 24 1.0397864704135884 1107 25 0.9598777237410303 1108 1 0.18899536456800803 1108 2 0.34636267488260586 1108 3 0.1507104853051935 1108 4 0.29469187138509767 1108 5 0.32432905204609597 1108 6 0.3291132607249594 1108 7 0.5925720911141283 1108 8 0.49307383394275206 1108 9 0.5812865028745051 1108 10 0.6145986907393919 1108 11 0.7429015449441181 1108 12 0.6183760213864247 1108 13 0.5182480222100949 1108 14 0.7615213171457924 1108 15 0.8118090460577037 1108 16 0.8321955556669849 1108 17 0.8258750048069814 1108 18 0.6461499027618132 1108 19 0.8134699247733224 1108 20 0.874704082692574 1108 21 1.0241097437874132 1108 22 0.934871084981495 1108 23 0.6776647665888618 1108 24 0.9090626175614958 1108 25 0.745656000898336 1109 1 -0.16225223619974471 1109 2 -0.11620930208476565 1109 3 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-0.7141158562814098 2314 5 -0.8107569106529979 2314 6 -0.6723615215819905 2314 7 -0.7871915822505073 2314 8 -0.9023449427831594 2314 9 -1.1100393914833688 2314 10 -2.2636004361991198 2314 11 -1.500060405913338 2314 12 -1.096956051634578 2314 13 -1.2664421951877642 2314 14 -1.1553101178872751 2314 15 -1.2460014564647324 2314 16 -1.1530204746383461 2314 17 -1.0831933400270017 2314 18 -1.1635405254913493 2314 19 -1.2276709591080532 2314 20 -1.0549100038882386 2314 21 -1.2509431808962717 2314 22 -1.1264311815323964 2314 23 -0.9805664826505591 2314 24 -1.184729440543002 2314 25 -1.0689744661281075 2315 1 -1.0954287243525798 2315 2 -1.1859368434249937 2315 3 -1.1516055573278088 2315 4 -1.3249747940644596 2315 5 -1.5106463683748572 2315 6 -1.3372763100847962 2315 7 -1.368228784882028 2315 8 -1.751961667188958 2315 9 -1.5716610789468746 2315 10 -1.7280264821412423 2315 11 -1.7198026915391402 2315 12 -1.757555830072345 2315 13 -1.5300749798699513 2315 14 -1.783800489324456 2315 15 -1.8351850318804779 2315 16 -2.033800806790623 2315 17 -2.147873509809113 2315 18 -2.073100064071877 2315 19 -1.98736550097858 2315 20 -2.2438385797239384 2315 21 -2.4597251817314074 2315 22 -2.1935407338262145 2315 23 -2.3558561237205353 2315 24 -2.1621644058975313 2315 25 -2.319190515179102 2316 1 -1.1959562632876413 2316 2 -1.3582738822354443 2316 3 -1.3566202037656794 2316 4 -1.3015918405394018 2316 5 -1.6230388369706965 2316 6 -1.5436677258570306 2316 7 -1.7628284927464364 2316 8 -2.469847103115125 2316 9 -2.1879824331068 2316 10 -2.055387414036243 2316 11 -1.9906580543646157 2316 12 -2.222066031231787 2316 13 -2.0899041235469795 2316 14 -2.0643608653352743 2316 15 -2.103660479006983 2316 16 -2.231579691290193 2316 17 -2.144008286259428 2316 18 -2.3274550676766097 2316 19 -2.186230605553355 2316 20 -1.9081681894904037 2316 21 -2.3095525428720336 2316 22 -2.4132790474632877 2316 23 -2.347072018100871 2316 24 -2.274784805377717 2316 25 -2.304397132855936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45656.0 3 2.0 4 753.0 5 6169.0 6 7643.0 7 6319.0 8 5135.0 9 4525.0 10 4324.0 11 4146.0 12 3851.0 13 3747.0 14 3733.0 15 3727.0 16 3783.0 17 3819.0 18 4120.0 19 4747.0 20 5562.0 21 7229.0 22 9273.0 23 11463.0 24 13503.0 25 16168.0 26 18463.0 27 21455.0 28 24446.0 29 29895.0 30 37071.0 31 47495.0 32 60356.0 33 77657.0 34 100357.0 35 135213.0 36 192500.0 37 288244.0 38 294782.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.0801962903547 21.130580766661804 14.48830055945015 23.300922383533347 2 15.949449722721818 22.40357293786169 39.78044636513148 21.866530974285013 3 20.075948812835403 25.876599101325453 30.430741489427337 23.616710596411803 4 13.949888909602471 18.99343939718615 37.96339357447037 29.093278118741004 5 13.148396028013352 38.61504738683866 35.84921687782418 12.387339707323806 6 31.22917262366395 38.84389029350554 17.7696869499798 12.157250132850713 7 27.369569125825716 32.22430906018652 22.5186107099237 17.88751110406407 8 24.58570811586838 38.48331919133886 20.477652221045012 16.453320471747745 9 26.31916944586159 15.620258589520153 20.280482521755342 37.780089442862916 10 14.530252479382433 30.449383712004863 34.70498516914998 20.315378639462732 11 34.848682870583836 22.759301042703957 24.483076378048352 17.90893970866386 12 22.98287502877603 25.225315474835984 32.34843574503543 19.443373751352556 13 29.317515529104092 22.308172524813727 25.92788179902092 22.446430147061264 14 20.655847985611654 22.677301800334497 28.206014471937486 28.460835742116362 15 22.944263735038952 31.483463154410014 23.775003632247994 21.79726947830304 16 22.035239771490136 28.930009400722206 27.93639884006897 21.09835198771869 17 21.551139066336457 28.89644013159684 28.151348310357843 21.401072491708856 18 21.27243452168104 28.136752975955513 30.22255894690682 20.36825355545663 19 23.306427055504066 27.28458447414669 28.05714206103371 21.351846409315538 20 23.11516183240443 27.96021577211641 28.683613619039217 20.241008776439944 21 22.94578961090829 27.170276468804794 27.828127995775315 22.055805924511603 22 22.584090687446885 28.350441940091457 28.248407284133346 20.81706008832831 23 22.41425406894703 27.996239711118527 28.090644987729966 21.498861232204472 24 22.72500200685848 27.435248130636204 28.816431162100425 21.02331870040489 25 22.78820962292103 27.70429450864694 28.556482232765006 20.951013635667024 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 175.0 1 175.0 2 325.0 3 475.0 4 475.0 5 475.0 6 1248.0 7 2021.0 8 2021.0 9 2021.0 10 2907.5 11 3794.0 12 3794.0 13 3794.0 14 5900.0 15 8006.0 16 8006.0 17 8006.0 18 14416.5 19 20827.0 20 20827.0 21 20827.0 22 32706.0 23 44585.0 24 44585.0 25 44585.0 26 63828.5 27 83072.0 28 83072.0 29 83072.0 30 105542.5 31 128013.0 32 128013.0 33 128013.0 34 141582.0 35 155151.0 36 155151.0 37 155151.0 38 164597.5 39 174044.0 40 174044.0 41 174044.0 42 180923.0 43 187802.0 44 187802.0 45 187802.0 46 189068.0 47 190334.0 48 190334.0 49 190334.0 50 181537.0 51 172740.0 52 172740.0 53 172740.0 54 152438.5 55 132137.0 56 132137.0 57 132137.0 58 112537.5 59 92938.0 60 92938.0 61 92938.0 62 75283.5 63 57629.0 64 57629.0 65 57629.0 66 44267.5 67 30906.0 68 30906.0 69 30906.0 70 22476.0 71 14046.0 72 14046.0 73 14046.0 74 9837.5 75 5629.0 76 5629.0 77 5629.0 78 3879.0 79 2129.0 80 2129.0 81 2129.0 82 1384.0 83 639.0 84 639.0 85 639.0 86 414.0 87 189.0 88 189.0 89 189.0 90 117.0 91 45.0 92 45.0 93 45.0 94 24.5 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.376692312438343 2 0.0 3 0.0 4 0.0 5 0.03171168111051919 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.002255642589451156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1507331.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.96349606362396 #Duplication Level Percentage of deduplicated Percentage of total 1 87.09557169398504 67.03179689229096 2 9.445681233291632 14.539453008333748 3 2.004001978345196 4.627049951155955 4 0.6419904917356019 1.9763933073430808 5 0.25456497817313495 0.9796105347782297 6 0.1359814985628832 0.627936691762209 7 0.0841428409730803 0.4533149047009662 8 0.0575611093887313 0.35440833726859605 9 0.039817449593455734 0.2758041112544529 >10 0.20418891482118337 2.86761973809311 >50 0.018201085407246808 0.9636525399960879 >100 0.014946852401935851 2.1828998908219743 >500 0.0021473546929099206 1.1269950304433802 >1k 0.0011166244403131589 1.6411104898379232 >5k 8.589418771639682E-5 0.3519545719193193 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5324 0.3532070925364104 No Hit TATCAACGCAGAGTACTTTTTTTTT 3611 0.23956251148553304 No Hit GGTATCAACGCAGAGTACTTTTTTT 3265 0.21660803101641243 No Hit GTCCTACAGTGGACATTTCTAAATT 2325 0.15424614766099815 No Hit CTGTAGGACGTGGAATATGGCAAGA 2319 0.15384809308638914 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2011 0.13341462492312572 No Hit CTTTAGGACGTGAAATATGGCGAGG 1912 0.12684672444207676 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1782 0.11822220865888115 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTTCC 35 0.0021736035 16.281847 9 GACCGTT 50 0.001502196 13.296844 7 CGTGCGC 65 5.464437E-5 13.1507225 10 TCCAACG 190 0.0 12.996914 18 CCAACGT 105 1.999797E-8 12.663659 19 GGTATCA 920 0.0 12.225688 1 ACTATCG 80 2.8731998E-5 11.872181 11 CGGTTTC 145 9.822543E-11 11.790303 13 GCGTGCG 65 8.0391753E-4 11.689531 9 ACCGTGC 60 0.0058859135 11.080702 8 GGCGAGG 600 0.0 11.080702 19 TAGGACC 430 0.0 11.046453 4 GCCGGTT 125 2.2465065E-7 10.637474 11 GACTGTG 325 0.0 10.22834 7 ATAACCG 75 0.0026476064 10.13328 4 GTATCGC 75 0.0026527122 10.130927 6 GTGCGCC 75 0.0026527122 10.130927 11 GCGCCAC 85 6.607107E-4 10.056435 13 ATCGGTC 85 6.607107E-4 10.056435 14 CCTATAC 95 1.6438343E-4 9.999948 3 >>END_MODULE