FastQCFastQC Report
Thu 26 May 2016
SRR1511729_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511729_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203309
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50470.22906455699132533No Hit
TATCAACGCAGAGTACTTTTTTTTT30970.14056131028375957No Hit
GGTATCAACGCAGAGTACTTTTTTT30560.13870047278888253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG751.5514714E-512.6080577
TAGGTCG550.003177598412.0349645
GTCTTAT2550.011.6356961
ATCGCGC907.859775E-611.5573858
GTATAGG2250.011.428841
TACCGTC752.1658547E-411.3472517
GCGCCTT1303.499008E-810.9108213
TCGCGCG1002.5255948E-510.4016489
GTCCTAG2800.010.2435691
AATCCCG1701.6898412E-910.01250719
TGGCGAG6150.09.99441818
TAGAACC1804.292815E-109.9813794
CGTGCGC951.7232975E-49.9537310
TAGACTG3050.09.9210955
GTCCTAC6300.09.8903421
CGCGCCT1151.0849246E-59.86717512
TCTTATA3050.09.7282062
GGTATCA12200.09.7282061
TCTAGAC2350.09.6572353
TAATACT2650.09.63445954
Produced by FastQC (version 0.11.5)