FastQCFastQC Report
Thu 26 May 2016
SRR1511729_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511729_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203309
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66420.30145567417007785No Hit
TATCAACGCAGAGTACTTTTTTTTT39950.18131819004960265No Hit
GGTATCAACGCAGAGTACTTTTTTT38230.17351174982719175No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC400.00529150714.2453378
CACGAAT550.00307667812.08695214
AGAACCG752.0734363E-411.4006675
GGGCGAT700.001495908810.85359111
GGTATCA15000.010.6862321
TAGGACC3650.010.6718584
ATTATAC1752.3646862E-1010.314893
GCGCCAC1303.860332E-710.22742113
ACCGAGT856.61515E-410.05553158
TGCACCG1054.1036816E-59.9529645
CTTAGGC1856.475602E-109.7573273
TAGGACA3900.09.744164
GTCTTAT2750.09.7147561
TATGGCG5700.09.66350316
GTATCAA28300.09.5749931
GTAGGAC6400.09.5005563
GGACCGT1201.7074624E-59.4968916
GATATAC4550.09.4364291
GTCCTAA4950.09.2521481
TCTTATA3200.09.2036642
Produced by FastQC (version 0.11.5)