FastQCFastQC Report
Thu 26 May 2016
SRR1511728_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511728_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335067
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52340.22414774394053788No Hit
GTCCTACAGTGGACATTTCTAAATT41620.17823899699666007No Hit
CTGTAGGACGTGGAATATGGCAAGA38840.16633355702427383No Hit
GTCCTAAAGTGTGTATTTCTCATTT37830.16200819933646443No Hit
CTTTAGGACGTGAAATATGGCGAGG35260.15100209116055344No Hit
GGTATCAACGCAGAGTACTTTTTTT31890.13656995709330824No Hit
TATCAACGCAGAGTACTTTTTTTTT30510.1306600624307568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5950.013.1916444
TGTCGAG1102.9431249E-912.89544618
TATGTCG1207.9671736E-1012.60888116
TCCAACG3100.012.20214418
ATGTCGA1251.5170372E-912.10452617
GGTATCA14250.012.0701971
CGACTAA1201.07247615E-811.82082617
CCAACGA1950.011.638967519
AGTCGTC1803.6379788E-1211.0327718
GCGACTA1303.4960976E-810.91153116
TAATACG700.001549881110.8076114
AAGTCGT1855.456968E-1210.7345887
GGCGAGG9850.010.46473119
GGACGTG24600.010.4177046
GGACCTG13450.010.4058436
AGGACGT25050.010.3815635
GTCCTAA12000.010.3793291
TGTAGGA21200.010.3513532
GTCGAGG1106.3873304E-610.31635719
CGGTAGG1156.397986E-610.3148611
Produced by FastQC (version 0.11.5)