##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511728_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2335067 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.912033359214103 31.0 28.0 34.0 16.0 34.0 2 26.297348213134782 31.0 25.0 34.0 10.0 34.0 3 27.942719844869547 31.0 27.0 34.0 16.0 34.0 4 32.373966143155634 35.0 32.0 37.0 22.0 37.0 5 32.812268770018164 35.0 33.0 37.0 26.0 37.0 6 33.051497879932356 36.0 35.0 37.0 26.0 37.0 7 33.07659780211874 37.0 35.0 37.0 26.0 37.0 8 33.088647991685036 37.0 35.0 37.0 26.0 37.0 9 34.63541902652044 39.0 35.0 39.0 27.0 39.0 10 34.50195690316381 39.0 35.0 39.0 27.0 39.0 11 34.500880274527454 39.0 35.0 39.0 27.0 39.0 12 34.40165228663675 39.0 35.0 39.0 26.0 39.0 13 34.39545717531874 39.0 35.0 39.0 26.0 39.0 14 35.48682971409386 40.0 36.0 41.0 26.0 41.0 15 35.422339059221855 40.0 36.0 41.0 26.0 41.0 16 35.3546926062507 40.0 36.0 41.0 25.0 41.0 17 35.3266514408366 40.0 36.0 41.0 25.0 41.0 18 35.254840225141294 40.0 36.0 41.0 25.0 41.0 19 35.27745156777086 40.0 36.0 41.0 25.0 41.0 20 35.20319759561503 40.0 36.0 41.0 25.0 41.0 21 35.112723103876675 40.0 36.0 41.0 24.0 41.0 22 34.99575558217387 40.0 35.0 41.0 24.0 41.0 23 34.87900903914106 40.0 35.0 41.0 22.0 41.0 24 34.75873111992075 39.0 35.0 41.0 21.0 41.0 25 34.64422476956764 39.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1733424651686626 1101 2 3.7418367462325257 1101 3 2.32769588757645 1101 4 1.3620043710315528 1101 5 0.989215715631687 1101 6 0.6186112195812328 1101 7 0.5012705231370234 1101 8 0.23252303866170365 1101 9 0.2917732332370164 1101 10 0.5488383162762247 1101 11 0.3305000115735197 1101 12 0.5521064589041629 1101 13 0.5532065726127513 1101 14 0.7141251823480843 1101 15 0.4991732988806561 1101 16 0.5353074205558457 1101 17 0.3837720762269399 1101 18 0.3723431426287078 1101 19 0.684640884551186 1101 20 0.7057865234977001 1101 21 0.6355504870355304 1101 22 0.6501631552278724 1101 23 0.5984390790544438 1101 24 0.7622128853890686 1101 25 0.5007786078993846 1102 1 1.898571087444136 1102 2 4.576804564170406 1102 3 3.1465490130248206 1102 4 2.1289944105763468 1102 5 1.7612647703072142 1102 6 1.4754613294558538 1102 7 1.3555903702942516 1102 8 1.3658710555066307 1102 9 1.5077615636210595 1102 10 1.5594129617952746 1102 11 1.4776909719003442 1102 12 1.5344432013293599 1102 13 1.603817616459395 1102 14 1.8335945118486023 1102 15 1.825760187931202 1102 16 1.74659014695672 1102 17 1.8876598505673812 1102 18 1.8112055312752489 1102 19 1.8761280557768671 1102 20 1.7595588854370305 1102 21 1.7588416409329994 1102 22 1.7229937922489498 1102 23 1.7514273414748018 1102 24 1.4786087461491064 1102 25 1.4934772946900168 1103 1 1.6988227367674043 1103 2 4.163737745931172 1103 3 2.7992063910137652 1103 4 1.869274334012772 1103 5 1.3990359734630289 1103 6 1.216236292777232 1103 7 1.0568223831973498 1103 8 1.0217644106042272 1103 9 1.0607837845477874 1103 10 1.0748787073671267 1103 11 1.1903229906331632 1103 12 1.2647810332587 1103 13 1.2242541648800298 1103 14 1.3498356093894373 1103 15 1.1836317593306305 1103 16 1.169917261928397 1103 17 1.3401792586570807 1103 18 1.4489976242397518 1103 19 1.489347904009982 1103 20 1.3553245697968777 1103 21 1.3012576687601651 1103 22 1.5517605162411385 1103 23 1.2477541358122082 1103 24 1.496738671042955 1103 25 1.1545144008199557 1104 1 1.5728713666830458 1104 2 4.01883697814905 1104 3 2.5845477699014268 1104 4 1.8297848144059117 1104 5 1.4614530942243462 1104 6 1.1714564311785978 1104 7 1.0709243901285603 1104 8 1.1271087982171935 1104 9 1.104340655027741 1104 10 1.10831456022931 1104 11 1.1569595700467943 1104 12 1.203049233260927 1104 13 1.0852064821556837 1104 14 1.2309408144842138 1104 15 1.3399378246979694 1104 16 1.1209558293294108 1104 17 1.264618465690809 1104 18 1.1305129074499547 1104 19 1.3103733221670808 1104 20 1.189407298599292 1104 21 1.409064753751089 1104 22 1.4105036491042213 1104 23 1.2514816121602834 1104 24 1.2874261090576482 1104 25 1.3327368013370133 1105 1 1.6207588936327006 1105 2 4.127291530979001 1105 3 2.6173206194960414 1105 4 1.8788275730837398 1105 5 1.4334004256692623 1105 6 1.2237823437555662 1105 7 1.182131303049033 1105 8 1.092025485166225 1105 9 1.2070242276929761 1105 10 1.0187601508431499 1105 11 0.9664408420876853 1105 12 1.2436506442230737 1105 13 1.1016891403020281 1105 14 1.1139758675894882 1105 15 1.2175889887331053 1105 16 1.1400083062808761 1105 17 1.2506702400956726 1105 18 1.1551011754395475 1105 19 1.2806428273291601 1105 20 1.188443670489832 1105 21 1.306276316943169 1105 22 1.3792001921261345 1105 23 1.372941476380639 1105 24 1.2658089917772415 1105 25 1.2848030996414082 1106 1 0.9360108108541993 1106 2 3.6228643273532697 1106 3 2.0746590251126342 1106 4 1.3125926792897076 1106 5 0.7953017096211923 1106 6 0.6708094714421549 1106 7 0.5651683366823335 1106 8 0.6021461897073408 1106 9 0.48597360724116356 1106 10 0.5006298015472836 1106 11 0.622122129860756 1106 12 0.4449686962147865 1106 13 0.6378857501272037 1106 14 0.7429580170541641 1106 15 0.8022981345628608 1106 16 0.7409166738553523 1106 17 0.6348270133109253 1106 18 0.8121868536558949 1106 19 0.7543154038668689 1106 20 0.779804021067477 1106 21 0.8223403476670796 1106 22 0.6408707565184173 1106 23 0.4921528701541149 1106 24 0.7033081755523867 1106 25 0.8504004915913725 1107 1 1.4513724072345227 1107 2 3.85901604486801 1107 3 2.48142364218036 1107 4 1.6296173368510836 1107 5 1.2474749016392934 1107 6 1.23912319324387 1107 7 0.9428208437736743 1107 8 0.9354123636518068 1107 9 1.0110531836914873 1107 10 1.0638261264271165 1107 11 1.0808314815168885 1107 12 1.064283125912766 1107 13 1.0655852852918244 1107 14 1.1807876467204608 1107 15 1.2751312542072313 1107 16 1.238674177308198 1107 17 1.134293368437234 1107 18 1.052968856259767 1107 19 1.129832341398732 1107 20 1.2460254886403845 1107 21 1.3127792928521487 1107 22 1.1551304108868266 1107 23 1.3742755193692417 1107 24 1.4372664047226635 1107 25 1.4659229734855685 1108 1 1.771332123738599 1108 2 4.238292059101948 1108 3 2.7753477672629145 1108 4 1.8616844453438048 1108 5 1.4566237895716583 1108 6 1.3659882763901905 1108 7 1.1684133495976923 1108 8 1.1030562720052117 1108 9 1.0716008223754727 1108 10 0.9813501043073316 1108 11 1.0566523481049117 1108 12 1.1890444971287906 1108 13 1.1252030520878407 1108 14 1.273615475847464 1108 15 1.3060708519637103 1108 16 1.3348395236444048 1108 17 1.3412112209917524 1108 18 1.3781143335666215 1108 19 1.2739164833044114 1108 20 1.5042932983156518 1108 21 1.521318671119431 1108 22 1.4012589566394809 1108 23 1.4554799498966418 1108 24 1.5572116638546305 1108 25 1.5792597349684954 1109 1 1.1752091643447713 1109 2 3.085610712220383 1109 3 1.8993450588017886 1109 4 1.321023450443647 1109 5 0.8628722153156687 1109 6 0.746315188240743 1109 7 0.6476998449260449 1109 8 0.4896705770656453 1109 9 0.6087117774191668 1109 10 0.4905141262239425 1109 11 0.521954264474509 1109 12 0.5734044676142744 1109 13 0.4016312912028752 1109 14 0.6413483397932254 1109 15 0.6474554860399451 1109 16 0.7077855825964008 1109 17 0.7178362235846407 1109 18 0.6060977153499962 1109 19 0.7095268204989296 1109 20 0.7356422541949996 1109 21 0.642390439412658 1109 22 0.6750246658621819 1109 23 0.6858717581352209 1109 24 0.6227836855104627 1109 25 0.7758787516845018 1110 1 1.107124566141799 1110 2 3.522988295682339 1110 3 2.1494646546822764 1110 4 1.2213030513787544 1110 5 0.7379163911525097 1110 6 0.4509041637570874 1110 7 0.37711303706333155 1110 8 0.3854131720150562 1110 9 0.3129874429728403 1110 10 0.51953513413428 1110 11 0.40165122344114224 1110 12 0.5239948789755005 1110 13 0.41386166237710853 1110 14 0.5206406862817587 1110 15 0.49610004291724863 1110 16 0.6701626973952557 1110 17 0.59494147559694 1110 18 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5 0.5261317810257538 1112 6 0.2714905223680475 1112 7 0.18425703565719687 1112 8 0.1707156161560377 1112 9 0.06855705483365426 1112 10 -0.023011841228829155 1112 11 0.18144803399199816 1112 12 0.04869782187821414 1112 13 -0.037302188295406324 1112 14 0.04674129579797892 1112 15 0.12401854755080421 1112 16 0.27098210795460886 1112 17 0.18284110358630556 1112 18 0.1791459329906786 1112 19 0.09879226101899974 1112 20 0.011630530100148917 1112 21 0.07570637535533109 1112 22 0.08775559271320077 1112 23 0.07808249628836705 1112 24 -0.0464610192007342 1112 25 -0.04229282285995595 1113 1 0.7655686697340727 1113 2 3.2589123519753436 1113 3 1.7258416061105173 1113 4 0.9121007338625802 1113 5 0.5708613233820259 1113 6 0.32357723289409535 1113 7 0.04878913770590998 1113 8 0.04617819923205957 1113 9 -0.029390902616860615 1113 10 -0.014157395883025004 1113 11 -0.047157221763441726 1113 12 0.015555733866179366 1113 13 0.09298673846210193 1113 14 0.07996688550733211 1113 15 -0.03779934504362359 1113 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-2.7297657439411296 2315 24 -2.7024728665981215 2315 25 -2.8889969067640493 2316 1 -0.6695993668453752 2316 2 1.5186549275955805 2316 3 0.2477874579061421 2316 4 -0.4903541590503835 2316 5 -0.8538234073544473 2316 6 -0.9730947834553163 2316 7 -1.335734723900277 2316 8 -1.4813822610095109 2316 9 -1.5111793391663255 2316 10 -1.7587119637982838 2316 11 -1.6422798768639453 2316 12 -1.9017303933140255 2316 13 -1.8065629262169196 2316 14 -2.0228601058551376 2316 15 -1.9534658564134233 2316 16 -2.1432899339974867 2316 17 -2.1437018596384476 2316 18 -2.3237585766679842 2316 19 -2.0437555966863457 2316 20 -2.075453807899052 2316 21 -2.380664396847777 2316 22 -2.108254015777618 2316 23 -2.3550982237728064 2316 24 -2.3597399296576214 2316 25 -2.296841985345253 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100824.0 3 3.0 4 887.0 5 4322.0 6 5269.0 7 4706.0 8 4277.0 9 4088.0 10 4031.0 11 3937.0 12 3813.0 13 3677.0 14 3882.0 15 3969.0 16 4156.0 17 4431.0 18 5112.0 19 6379.0 20 7918.0 21 10541.0 22 12720.0 23 15316.0 24 17724.0 25 20043.0 26 22320.0 27 25553.0 28 29237.0 29 35634.0 30 43927.0 31 54703.0 32 69465.0 33 90899.0 34 122235.0 35 172273.0 36 267143.0 37 340807.0 38 808846.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04231955656947 18.651970157601475 13.10857461477551 25.19713567105355 2 16.35075078516407 21.006720001718946 39.97501262350443 22.667516589612557 3 18.8308943597764 24.42443835658677 29.631997711414705 27.112669572222124 4 12.227957484731702 17.07968122542094 38.520008205332005 32.17235308451535 5 13.257178487812126 37.607015130615096 35.901539441908945 13.23426693966383 6 31.480938234320472 37.228053841709894 18.408465367374898 12.882542556594737 7 27.697106763960093 31.54324908021911 22.423082506840274 18.33656164898052 8 24.672996535003065 37.330192238595295 20.836489916563423 17.16032130983822 9 26.276676429412948 15.489876735870961 20.337403594843316 37.896043239872775 10 15.031988375494151 29.063748492013293 34.445949516651986 21.458313615840574 11 34.28878057888703 22.36950802696454 24.787725577039115 18.553985817109318 12 23.365796356164513 24.345382809144233 32.001137440595926 20.28768339409533 13 29.0940688211516 21.916801530748366 25.87938590198911 23.109743746110926 14 20.8394448638947 22.20484465756229 27.797275195957976 29.15843528258504 15 22.921869051294887 30.900055544444765 23.56287849556351 22.615196908696838 16 22.116324713594942 27.96168161341837 27.93919831850649 21.982795354480192 17 21.723231067887987 28.273535620177064 27.973972481303534 22.02926083063141 18 21.41201944098392 27.519424496170775 29.937727696892637 21.13082836595267 19 23.445589974758743 26.7816841151385 27.53772270248464 22.235003207618114 20 23.467335198518928 27.396901245231938 28.139663658473186 20.996099897775952 21 23.022343732200685 26.73513594071592 27.266684995944434 22.975835331138956 22 23.026853363436874 27.99809169131994 27.48970900862165 21.48534593662154 23 22.496639259699254 27.609718160331404 27.734083752346304 22.159558827623037 24 23.06310696866514 26.704844015182434 28.442181744677992 21.789867271474435 25 22.889664407916346 27.17652212977186 28.078766048254717 21.85504741405707 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 238.0 1 238.0 2 396.5 3 555.0 4 555.0 5 555.0 6 1379.5 7 2204.0 8 2204.0 9 2204.0 10 3208.5 11 4213.0 12 4213.0 13 4213.0 14 6964.0 15 9715.0 16 9715.0 17 9715.0 18 17961.5 19 26208.0 20 26208.0 21 26208.0 22 42310.0 23 58412.0 24 58412.0 25 58412.0 26 85316.5 27 112221.0 28 112221.0 29 112221.0 30 146956.0 31 181691.0 32 181691.0 33 181691.0 34 203583.5 35 225476.0 36 225476.0 37 225476.0 38 242175.0 39 258874.0 40 258874.0 41 258874.0 42 274144.5 43 289415.0 44 289415.0 45 289415.0 46 296315.5 47 303216.0 48 303216.0 49 303216.0 50 292745.5 51 282275.0 52 282275.0 53 282275.0 54 252049.5 55 221824.0 56 221824.0 57 221824.0 58 190286.0 59 158748.0 60 158748.0 61 158748.0 62 130624.5 63 102501.0 64 102501.0 65 102501.0 66 79026.0 67 55551.0 68 55551.0 69 55551.0 70 40756.5 71 25962.0 72 25962.0 73 25962.0 74 18188.5 75 10415.0 76 10415.0 77 10415.0 78 7024.5 79 3634.0 80 3634.0 81 3634.0 82 2425.0 83 1216.0 84 1216.0 85 1216.0 86 783.5 87 351.0 88 351.0 89 351.0 90 229.5 91 108.0 92 108.0 93 108.0 94 72.0 95 36.0 96 36.0 97 36.0 98 22.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.331310407795579 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.282532364167709E-5 20 0.0 21 2.1412661820838547E-4 22 6.423798546251563E-4 23 6.852051782668334E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2335067.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.34948379590443 #Duplication Level Percentage of deduplicated Percentage of total 1 80.62823375898672 55.91526390561244 2 13.060795275268411 18.115188206077036 3 3.53915915447431 7.36316581303029 4 1.2777252127425773 3.544383357468397 5 0.5550352560844605 1.924570424899248 6 0.2777563924273953 1.1557357461511515 7 0.15717014520996464 0.7629767906906871 8 0.10219605641228267 0.5669795006535145 9 0.0700489560058529 0.43720730455031265 >10 0.2891725914525121 3.508209603105019 >50 0.021718114400552133 1.023158992180649 >100 0.01723397009687624 2.4199385490252907 >500 0.0022530698628214943 1.1002550082632188 >1k 0.0014394613012444141 1.935797776074191 >5k 6.258527396714846E-5 0.22716902221861202 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5234 0.22414774394053788 No Hit GTCCTACAGTGGACATTTCTAAATT 4162 0.17823899699666007 No Hit CTGTAGGACGTGGAATATGGCAAGA 3884 0.16633355702427383 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3783 0.16200819933646443 No Hit CTTTAGGACGTGAAATATGGCGAGG 3526 0.15100209116055344 No Hit GGTATCAACGCAGAGTACTTTTTTT 3189 0.13656995709330824 No Hit TATCAACGCAGAGTACTTTTTTTTT 3051 0.1306600624307568 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.282532364167709E-5 2 0.0 0.0 0.0 0.0 4.282532364167709E-5 3 0.0 0.0 0.0 0.0 4.282532364167709E-5 4 0.0 0.0 0.0 0.0 4.282532364167709E-5 5 0.0 0.0 0.0 0.0 4.282532364167709E-5 6 0.0 0.0 0.0 0.0 4.282532364167709E-5 7 0.0 0.0 0.0 0.0 4.282532364167709E-5 8 0.0 0.0 0.0 0.0 4.282532364167709E-5 9 0.0 0.0 0.0 8.565064728335418E-5 4.282532364167709E-5 10 0.0 0.0 0.0 8.565064728335418E-5 4.282532364167709E-5 11 0.0 0.0 0.0 1.2847597092503128E-4 4.282532364167709E-5 12 0.0 0.0 0.0 1.2847597092503128E-4 4.282532364167709E-5 13 0.0 0.0 0.0 1.2847597092503128E-4 4.282532364167709E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 595 0.0 13.191644 4 TGTCGAG 110 2.9431249E-9 12.895446 18 TATGTCG 120 7.9671736E-10 12.608881 16 TCCAACG 310 0.0 12.202144 18 ATGTCGA 125 1.5170372E-9 12.104526 17 GGTATCA 1425 0.0 12.070197 1 CGACTAA 120 1.07247615E-8 11.820826 17 CCAACGA 195 0.0 11.6389675 19 AGTCGTC 180 3.6379788E-12 11.032771 8 GCGACTA 130 3.4960976E-8 10.911531 16 TAATACG 70 0.0015498811 10.807611 4 AAGTCGT 185 5.456968E-12 10.734588 7 GGCGAGG 985 0.0 10.464731 19 GGACGTG 2460 0.0 10.417704 6 GGACCTG 1345 0.0 10.405843 6 AGGACGT 2505 0.0 10.381563 5 GTCCTAA 1200 0.0 10.379329 1 TGTAGGA 2120 0.0 10.351353 2 GTCGAGG 110 6.3873304E-6 10.316357 19 CGGTAGG 115 6.397986E-6 10.314861 1 >>END_MODULE