FastQCFastQC Report
Thu 26 May 2016
SRR1511728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511728_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335067
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69840.2990920603134728No Hit
GTCCTACAGTGGACATTTCTAAATT47360.20282073276698273No Hit
TATCAACGCAGAGTACTTTTTTTTT43800.18757491755054567No Hit
CTGTAGGACGTGGAATATGGCAAGA43770.18744644157962062No Hit
GGTATCAACGCAGAGTACTTTTTTT42130.1804230885023856No Hit
GTCCTAAAGTGTGTATTTCTCATTT41870.17930963008770198No Hit
CTTTAGGACGTGAAATATGGCGAGG39810.1704876134175165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2500.013.67688519
TCCAACG3750.012.1564518
TAGGACC7600.012.1245774
TAAGACG550.003070042212.09048754
GGTATCA12350.011.58669951
CGTGCGC600.005889940511.08009810
TGGACGG700.001492323410.8567645
GTCCTAC22150.010.6810361
GGCGAGG9100.010.64592819
AGGACCT16950.010.5365065
TGGCGAG21800.010.28140218
GTATCAA26350.010.2094871
GTCCTAA12650.010.1807231
AGGACGT24350.010.1043695
GGACCTG15850.010.0065426
CTAGGAC3450.09.9126973
GGACGTG24600.09.84468656
ACCGTGC1451.6247941E-79.82471758
GTCCTAT1951.5097612E-109.7843251
TTAGGAC13900.09.773043
Produced by FastQC (version 0.11.5)