FastQCFastQC Report
Thu 26 May 2016
SRR1511727_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511727_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114666
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44513.881708614584969No Hit
GGTATCAACGCAGAGTACTTTTTTT27192.37123471648091No Hit
TATCAACGCAGAGTACTTTTTTTTT26232.2875132994959273No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12011.0473898104058745No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9510.8293652870074827No Hit
GTACTGGTTCACTATCGGTCAGTCA8080.7046552596236024No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7710.6723876301606405No Hit
ATACAGGGTGACAGCCCCGTACACA5640.49186332478677197No Hit
GTGGTATCAACGCAGAGTACATGGG5100.4447700277327194No Hit
ATCATTAACTGAATCCATAGGTTAA5100.4447700277327194No Hit
GTACATAAGCAGTGGTATCAACGCA4880.4255838696736609No Hit
GGTATCAACGCAGAGTACATGGGGG4550.3968046325850732No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4200.3662811993092983No Hit
GGATACCACGTGTCCCGCCCTACTC4170.3636649050285176No Hit
GTGGTATCAACGCAGAGTACTTTTT3760.3279088831911814No Hit
ATCAACGCAGAGTACTTTTTTTTTT3690.3218041965360264No Hit
GTATCAACGCAGAGTACATGGGGGT3470.3026180384769679No Hit
TATTCAGACAGGATACCACGTGTCC3380.2947691556346258No Hit
GTACATGGGGGTTAAGCGACTAAGC3110.27122250710759943No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2930.25552474142291526No Hit
CTATCGGTCAGTCAGGAGTATTTAG2880.2511642509549474No Hit
GTATTTAGCCTTGGAGGATGGTCCC2820.24593166239338599No Hit
CCCATATTCAGACAGGATACCACGT2760.2406990738318246No Hit
GTTCACTATCGGTCAGTCAGGAGTA2750.23982697573823103No Hit
GGTTAATGAGGCGAACCGGGGGAAC2580.22500130814714037No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG2440.21279193483683045No Hit
GGTATCAACGCAGAGTACATGGGGA2430.2119198367432369No Hit
GCCCAGAGCCTGAATCAGTGTGTGT2390.2084314443688626No Hit
GTCAGGAGTATTTAGCCTTGGAGGA2360.20581515008808193No Hit
AGCGTACACGGTGGATGCCCTGGCA2300.20058256152652051No Hit
ACTTAGATGTTTCAGTTCCCCCGGT2270.1979662672457398No Hit
TCTAAGTACCCCGAGGAAAAGAAAT2140.18662899202902344No Hit
GTTAATGATAGTGTGTCGAAACACA2110.18401269774824272No Hit
TCACGGTACTGGTTCACTATCGGTC2080.181396403467462No Hit
CGGTACTGGTTCACTATCGGTCAGT2050.1787801091866813No Hit
ACCCTGTATCGCGCGCCTTTCCAGA2020.1761638149059006No Hit
GGTTCACTATCGGTCAGTCAGGAGT1970.17180332443793278No Hit
GCCTTGGAGGATGGTCCCCCCATAT1970.17180332443793278No Hit
GTGGATGCCCTGGCAGTCAGAGGCG1940.16918703015715209No Hit
GCTTATGTACTCTGCGTTGATACCA1940.16918703015715209No Hit
GNATCAACGCAGAGTACTTTTTTTT1920.16744283396996495No Hit
GAATCAGTGTGTGTGTTAGTGGAAG1900.1656986377827778No Hit
GGATCAACGCAGAGTACTTTTTTTT1800.15697765684684215No Hit
TATCAACGCAGAGTACATGGGGGTT1770.15436136256606142No Hit
GAATCAACGCAGAGTACTTTTTTTT1710.14912877400450003No Hit
GCCTGAATCAGTGTGTGTGTTAGTG1700.14825667591090644No Hit
ACGCAGAGTACATGGGGGTTAAGCG1700.14825667591090644No Hit
CACACACACTGATTCAGGCTCTGGG1580.13779149878778366No Hit
GTTATAACCGGCGATTTCCGAATGG1540.1343031064134094No Hit
GAGTACATGGGGGTTAAGCGACTAA1540.1343031064134094No Hit
CACTATCATTAACTGAATCCATAGG1520.13255891022622224No Hit
GTACTTAGATGTTTCAGTTCCCCCG1490.12994261594544154No Hit
GGTGATATGAACCGTTATAACCGGC1490.12994261594544154No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG1490.12994261594544154No Hit
CAACGCAGAGTACTTTTTTTTTTTT1480.12907051785184798No Hit
GTGCTAATCTGCGATAAGCGTCGGT1470.1281984197582544No Hit
GGGTGACAGCCCCGTACACAAAAAT1470.1281984197582544No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA1470.1281984197582544No Hit
GTATCAACGCAGAGTACATGGGGAA1450.12645422357106728No Hit
GNTATCAACGCAGAGTACTTTTTTT1410.12296583119669299No Hit
GGAGTATTTAGCCTTGGAGGATGGT1370.11947743882231873No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA1340.11686114454153804No Hit
GGTATCAACGCAGAGTACATGGGCA1330.11598904644794447No Hit
CATCTAAGTACCCCGAGGAAAAGAA1300.11337275216716378No Hit
GAGTACATAAGCAGTGGTATCAACG1300.11337275216716378No Hit
CCGTTATAACCGGCGATTTCCGAAT1290.11250065407357018No Hit
GATATGAACCGTTATAACCGGCGAT1280.11162855597997663No Hit
GAGTAAGCAGTGGTATCAACGCAGA1280.11162855597997663No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1250.10901226169919592No Hit
GTATCAACGCAGAGTACATGGGGGG1250.10901226169919592No Hit
TNTCAACGCAGAGTACTTTTTTTTT1240.10814016360560237No Hit
CTATCATTAACTGAATCCATAGGTT1240.10814016360560237No Hit
TCATTAACCTATGGATTCAGTTAAT1230.10726806551200879No Hit
TCAACGCAGAGTACTTTTTTTTTTT1220.10639596741841523No Hit
GGTATCAACGCAGAGTACATGGGGT1220.10639596741841523No Hit
CTTATGTACTCTGCGTTGATACCAC1170.10203547695044739No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1170.10203547695044739No Hit
AACGCAGAGTACTTTTTTTTTTTTT1170.10203547695044739No Hit
GTCCCGCCCTACTCATCGAGCTCAC1170.10203547695044739No Hit
TCGGTAAGGTGATATGAACCGTTAT1160.10116337885685382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCAA357.2780786E-519.7589971
GATCAAC402.025663E-417.2891222
TACATAA1259.826181E-911.8553982
AGCGTAC751.4173692E-411.8553981
CGCGCCT658.229932E-411.63970712
GGTATCA9450.011.4999461
GTACATA1301.7556886E-811.3994221
ATCATTA1155.1486313E-711.1681281
ACCGGGG600.00601823311.03347115
ACATAAG1201.3094723E-711.0334713
GCGCCTT700.001529708910.80829813
CCGGGGG700.001529708910.80829816
TGAATCC1408.554707E-910.80829810
CATAAGC1252.3000575E-710.5921334
CAGCCCC909.775968E-510.50806912
GACAGCC909.775968E-510.50806910
CCCCGTA909.775968E-510.50806915
CCGTACA909.775968E-510.50806917
GTGACAG909.775968E-510.5080698
GAATCCA1355.833681E-810.50806811
Produced by FastQC (version 0.11.5)