FastQCFastQC Report
Thu 26 May 2016
SRR1511727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114666
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57755.036366490502852No Hit
GGTATCAACGCAGAGTACTTTTTTT37343.2564142814783805No Hit
TATCAACGCAGAGTACTTTTTTTTT34362.9965290495874974No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16781.4633806010500061No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12931.1276228350164827No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9740.8494235431601346No Hit
GTACTGGTTCACTATCGGTCAGTCA9120.7953534613573334No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6070.5293635428112955No Hit
ATACAGGGTGACAGCCCCGTACACA5840.5093052866586434No Hit
ATCATTAACTGAATCCATAGGTTAA5690.4962238152547398No Hit
GTGGTATCAACGCAGAGTACATGGG5450.47529346100849423No Hit
GTACATAAGCAGTGGTATCAACGCA5310.46308408769818427No Hit
GTGGTATCAACGCAGAGTACTTTTT5040.43953743917115795No Hit
ATCAACGCAGAGTACTTTTTTTTTT4990.4351769487031901No Hit
GGTATCAACGCAGAGTACATGGGGG4880.4255838696736609No Hit
GGATACCACGTGTCCCGCCCTACTC4290.37413008215164045No Hit
GTATCAACGCAGAGTACATGGGGGT3660.3191879022552457No Hit
TATTCAGACAGGATACCACGTGTCC3470.3026180384769679No Hit
GTATTTAGCCTTGGAGGATGGTCCC3190.278199291856348No Hit
GTTCACTATCGGTCAGTCAGGAGTA3150.27471089948197375No Hit
GTACATGGGGGTTAAGCGACTAAGC3110.27122250710759943No Hit
GGTTAATGAGGCGAACCGGGGGAAC2980.2598852318908831No Hit
CTATCGGTCAGTCAGGAGTATTTAG2960.25814103570369595No Hit
CCCATATTCAGACAGGATACCACGT2830.2468037604869796No Hit
GCTTATGTACTCTGCGTTGATACCA2830.2468037604869796No Hit
TCACGGTACTGGTTCACTATCGGTC2680.23372228908307607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2670.2328501909894825No Hit
GTCAGGAGTATTTAGCCTTGGAGGA2660.23197809289588894No Hit
GGTATCAACGCAGAGTACATGGGGA2660.23197809289588894No Hit
TCTAAGTACCCCGAGGAAAAGAAAT2640.23023389670870179No Hit
ACTTAGATGTTTCAGTTCCCCCGGT2640.23023389670870179No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG2580.22500130814714037No Hit
AGCGTACACGGTGGATGCCCTGGCA2480.21628032721120474No Hit
ACCCTGTATCGCGCGCCTTTCCAGA2460.21453613102401758No Hit
GCCCAGAGCCTGAATCAGTGTGTGT2380.20755934627526904No Hit
GTGGATGCCCTGGCAGTCAGAGGCG2330.2031988558073012No Hit
CGGTACTGGTTCACTATCGGTCAGT2320.20232675771370764No Hit
GTTAATGATAGTGTGTCGAAACACA2320.20232675771370764No Hit
GGTTCACTATCGGTCAGTCAGGAGT2200.19186158059058483No Hit
GCCTTGGAGGATGGTCCCCCCATAT2090.1822685015610556No Hit
GTTATAACCGGCGATTTCCGAATGG2000.17441961871871348No Hit
TATCAACGCAGAGTACATGGGGGTT1990.1735475206251199No Hit
ACGCAGAGTACATGGGGGTTAAGCG1980.17267542253152635No Hit
GCCTGAATCAGTGTGTGTGTTAGTG1860.16221024540840354No Hit
GAATCAGTGTGTGTGTTAGTGGAAG1780.155233460659655No Hit
CACACACACTGATTCAGGCTCTGGG1740.15174506828528073No Hit
CACTATCATTAACTGAATCCATAGG1720.1500008720980936No Hit
GGTATCAACGCAGAGTACATGGGCA1700.14825667591090644No Hit
GAGTACATGGGGGTTAAGCGACTAA1680.14651247972371934No Hit
AACGCAGAGTACTTTTTTTTTTTTT1680.14651247972371934No Hit
GAGTACATAAGCAGTGGTATCAACG1660.14476828353653218No Hit
CATCTAAGTACCCCGAGGAAAAGAA1660.14476828353653218No Hit
CTTATACACATCTCCGAGCCCACGA1620.14127989116215792No Hit
CAACGCAGAGTACTTTTTTTTTTTT1620.14127989116215792No Hit
GGTGATATGAACCGTTATAACCGGC1600.1395356949749708No Hit
GTATCAACGCAGAGTACATGGGGAA1600.1395356949749708No Hit
GATATGAACCGTTATAACCGGCGAT1590.13866359688137722No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG1590.13866359688137722No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA1570.1369194006941901No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1540.1343031064134094No Hit
CTATCATTAACTGAATCCATAGGTT1540.1343031064134094No Hit
TCATTAACCTATGGATTCAGTTAAT1500.1308147140390351No Hit
TCAACGCAGAGTACTTTTTTTTTTT1500.1308147140390351No Hit
GTGCTAATCTGCGATAAGCGTCGGT1490.12994261594544154No Hit
GTACTTAGATGTTTCAGTTCCCCCG1480.12907051785184798No Hit
CTTATGTACTCTGCGTTGATACCAC1460.12732632166466085No Hit
ATCCATAGGTTAATGAGGCGAACCG1430.12471002738388014No Hit
GCGTACACGGTGGATGCCCTGGCAG1400.12209373310309943No Hit
GTATCAACGCAGAGTACATGGGGGA1400.12209373310309943No Hit
GGTATCAACGCAGAGTACATGGGGT1380.1203495369159123No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA1350.11773324263513159No Hit
GTATCAACGCAGAGTACATGGGGAG1350.11773324263513159No Hit
GGTATCAACGCAGAGTACATGGGAT1310.11424485026075733No Hit
TTTGTGTACGGGGCTGTCACCCTGT1300.11337275216716378No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1290.11250065407357018No Hit
GGAGTATTTAGCCTTGGAGGATGGT1280.11162855597997663No Hit
CCGTTATAACCGGCGATTTCCGAAT1270.11075645788638307No Hit
TCGGTAAGGTGATATGAACCGTTAT1260.10988435979278949No Hit
GATTCAGGCTCTGGGCTGCTCCCCG1240.10814016360560237No Hit
GTATCAACGCAGAGTACATGGGCAT1240.10814016360560237No Hit
GAGTAAGCAGTGGTATCAACGCAGA1230.10726806551200879No Hit
AGCTCACAGCATGTGCATTTTTGTG1210.10552386932482166No Hit
CCTATGGATTCAGTTAATGATAGTG1210.10552386932482166No Hit
GTATCAACGCAGAGTACATGGGGGG1190.10377967313763452No Hit
GGGTACTTAGATGTTTCAGTTCCCC1190.10377967313763452No Hit
GGTATCAACGCAGAGTACATGGGAA1150.10029128076326026No Hit
GGGTGACAGCCCCGTACACAAAAAT1150.10029128076326026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGCAG657.9458416E-411.688556
GGGGAAC657.9458416E-411.6885519
GGTATCA11100.011.53521251
AACCGGG600.00583354511.07977114
CGGGGGA600.00583354511.07977117
GAACCGG600.00583354511.07977113
CCGGGGG600.00583354511.07977116
GGGCAGT700.001477192610.8536547
TCTAAGT803.5192783E-410.7496991
GTACTGG2001.8189894E-1210.5108171
TACTGGT2100.010.401422
ACTGGTT2100.010.401423
GACAGGA1201.4835987E-610.2883597
AGACAGG1201.4835987E-610.2883596
CCGTACA856.513525E-410.05559217
TGTCCCG951.6191365E-49.99678611
TGAATCC1054.0290997E-59.94918310
ACAGGAT1252.5027603E-69.8768258
TGGTTCA2159.094947E-129.7178065
CTGGTTC2251.8189894E-129.707994
Produced by FastQC (version 0.11.5)