FastQCFastQC Report
Thu 26 May 2016
SRR1511726_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511726_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316251
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59360.25627619804589397No Hit
GTCCTACAGTGGACATTTCTAAATT39900.17226112368650895No Hit
CTGTAGGACGTGGAATATGGCAAGA38160.1647489844580747No Hit
TATCAACGCAGAGTACTTTTTTTTT35770.15443058632246676No Hit
GGTATCAACGCAGAGTACTTTTTTT34850.15045865063846708No Hit
GTCCTAAAGTGTGTATTTCTCATTT33330.14389632211707626No Hit
CTTTAGGACGTGAAATATGGCGAGG28950.12498645440412115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC457.003181E-414.7102174
GTCAACG500.001110686813.8403122
TACGCCT550.003176362512.0356324
CCAACGA1900.011.94513919
TAGGACC6600.011.8923514
GGCGAGG8750.011.67210719
ACTGCGC658.3404843E-411.6388538
TGGACCG658.3404843E-411.6388535
TCCAACG3500.011.6180718
CTACGAC600.00608099511.0326633
ATGTCGA1502.3028406E-910.717442517
GCCGGTT2300.010.69003311
CCCGTTT2400.010.63863912
TTAGGAC13800.010.6215083
GTCCTAC20800.010.5513361
AGGACGT22750.010.5165355
CGGTTTC2700.010.507297513
GGACGTG22300.010.4743156
GACGTGA11800.010.4182527
GGTATCA11600.010.3972791
Produced by FastQC (version 0.11.5)